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Clone: HP10A09

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2028
  tissue: epidermis
  stage/tissue: seedlings grown in greenhouse for 6 days
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp

Related with
LOC_Os09g30340.1 annotated gene (TIGR5)
LOC_Os09g30350.1 annotated gene (TIGR5)
Hv.23072 unigene cluster
Os.12127 unigene cluster
Zm.80765 unigene cluster
Sbi.12403 unigene cluster
LOC_Os09g30350.1 annotated gene (MSU 6.1)
LOC_Os09g30340.1 annotated gene (MSU 6.1)
 
Sequence: HP10A09T
submitter of sequence: Dr. Nils Stein
length: 348
priming: 5-prime
GenBankAc: CB878847
IPK CR-EST: HP10A09T
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HP10A09T
GGTCCCCGTA GCAGAACGGC AAGACCACTT CGAGGCCGGC GACGAGCGCG CCAAGGAGTT         60
CGCCGGGATC CTCAAGTCCA ACGACCCCGT CGGCTTCAAC CTCGTCGACG TCCTCGCATG        120
GGGCTCCATC GGCCACATCG TCGCCTACTA CATCCTCGCC ACCACCAGCA ACGGATACGA        180
CCCTCCCTTC TTCGGCTGAT CCGTCCGTCT CCCCTGCCTG CCTGCGTGCC GGCCGGAGCG        240
GCTTTGCTGT TGTAATCCGC CGTGTTTTCA TTAGTTCTGT ATACGCTGAT GATGTACCAT        300
CATGAGTATG TATGTATGTA TGTGAGATGG TGAAAGATTG ATCTGGAC                     348
TypePositionOrientation
ORF 1 - 252: forward
ORF 2 - 196: forward
ORF 138 - 1: reverse
ORF 271 - 2: reverse
ORF 348 - 274: reverse
Cluster Info:
Contig40620.1 (29 members)
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