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Clone: HP08H05

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2028
  tissue: epidermis
  stage/tissue: seedlings grown in greenhouse for 6 days
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp

Related with
Hv.23072 unigene cluster
 
Sequence: HP08H05T
submitter of sequence: Dr. Nils Stein
length: 658
priming: 5-prime
GenBankAc: CB878410
IPK CR-EST: HP08H05T
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HP08H05T
CGGCACGAGG CGAGATCAAG GACTCGCGGC GCGTTGGTCC CTGCAGCACC CACCCACCCA         60
CACAGCTCCA GCTCCGAAGG AGGAGTGAGG ACCATGGCCA CCTCCACCGC CGCCGTGCTG        120
TCCCCGCCCG CCGTGGCCGG GCTCCGCCTA GCGCCGTCCC CGCGCGCGGC GGCCTCGTTC        180
CGGGGGGTCT CACCGCCGGC GCGGCGGTCC GTGGCGGCGC GGGCCGCGCT GGAGCCGTCG        240
GTGGTGATCA GCCTCAGCAC GGGGCTGTCG CTCGTGATGG GCCGCTTCGT CTTCTTCAAC        300
TTCCAGCGGG AGAACGTGGC GAAGCAGGTC CCCGAGCAGA ACGGCAAGAC CCACTTCGAG        360
GCCGGCGACG AGCGCGCCAA GGAGTTCGCC GGGATCCTCA AGTCCAACGA CCCCGTCGGC        420
TTCAACCTCG TCGACGTCCT CGCATGGGGC TCCATCGGCC ACATCGTCGC CTACTACATC        480
CTCGCCACCA CCAGCAACGG ATACGACCCT CCCTTCTTCG GCTGATCCGT CCGTCTCCCC        540
TGCCTGACTG CGTGCCGGCC GGAGCGGCTT TGCTGTTGTA ATCCGCCGTG TTTTCATTAG        600
TTCTGTATAC GCTGATGATG TACCATCATG AGTATGTATG TATGTATGTG AGATGGTG          658
TypePositionOrientation
ORF 1 - 522: forward
ORF 2 - 85: forward
ORF 3 - 578: forward
ORF 464 - 3: reverse
ORF 657 - 193: reverse
Cluster Info:
Contig40620.1 (29 members)
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