•Home    •Contact   •FAQs   •Imprint   •Login  
 
 
 
 
 
 
 
 
 
   
 
   
 
  
 
   

Help us improve the primary database. What do you think and where should we go in the future: https://www.surveymonkey.com/s/RK3GMPC?

 search 
 for 
Help

Clone: HP06K11

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2028
  tissue: epidermis
  stage/tissue: seedlings grown in greenhouse for 6 days
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp

Related with
LOC_Os09g30340.1 annotated gene (TIGR5)
LOC_Os09g30350.1 annotated gene (TIGR5)
Hv.23072 unigene cluster
Zm.80765 unigene cluster
Sbi.12403 unigene cluster
Os.12127 unigene cluster
Sof.11976 unigene cluster
Vvi.6924 unigene cluster
LOC_Os09g30350.1 annotated gene (MSU 6.1)
LOC_Os09g30340.1 annotated gene (MSU 6.1)
 
Sequence: HP06K11T
submitter of sequence: Dr. Nils Stein
length: 386
priming: 5-prime
GenBankAc: CB877915
IPK CR-EST: HP06K11T
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HP06K11T
CGGCACGAGG GTCTTCTTCT ACTTCCAGCG GGAGAACGTG GCGAAGCAGG TCCCCGAGCA         60
GAACGGCAAG ACCCACTTCG AGGCCGGCGA CGAGCGCGCC AAGGAGTTCG CCGGGATCCT        120
CAAGTCCAAC GACCCCGTCG GCTTCAACCT CGTCGACGTC CTCGCATGGG GCTCCATCGG        180
CCACATCGTC GCCTACTACA TCCTCGCCAC CACCAGCAAC GGATACGACC CTCCCTTCTT        240
CGGCTGATCC GTCCGTCTCC CCTGCCTGCC TGCGTGCCGG CCGGAGCGGC TTTGCTGTTG        300
TAATCCGCCG TGTTTTCATT AGTTCTGTAT ACGCTGATGA TGTACCATCA TGAGTATGTA        360
TGTATGTATG TGAGATGGTG AAAGAT                                             386
TypePositionOrientation
ORF 1 - 300: forward
ORF 2 - 244: forward
ORF 3 - 350: forward
ORF 186 - 22: reverse
ORF 319 - 2: reverse
ORF 384 - 322: reverse
Cluster Info:
Contig40620.1 (29 members)
view Blast Searches
GabiPD Webmaster