|
|
Clone: HP04M21
| |
species: |
Hordeum vulgare |
| |
type: |
wildtype |
| |
common name: |
barley |
| |
cultivar: |
Barke |
| |
lib name: |
IPKc 2028 |
| |
tissue: |
epidermis |
| |
stage/tissue: |
seedlings grown in greenhouse for 6 days |
| |
cloning vector: |
pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA) |
| |
host: |
XL10-Gold |
| |
antibiotic: |
Ampicillin |
| |
library description: |
| |
Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp
|
| |
|
|
HP04M21T
CAGCTCCGAA GGAGGAGTGA GGACCATGGC CACCTCCACC GCCGCCGTGC TGTCCCCGCC 60
CGCCGTGGGC GGGCTCCGCC TAGCGCCGTC CCCGCGCGCG GCGGTCTCGT TCCGGGGGGT 120
CTCACCGCCG GCGCGGCGGT CCGTGGCGG 149
|
|
|
|
|
|
|
|
|
|
|
