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Clone: HM14O03
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species: |
Hordeum vulgare |
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type: |
wildtype |
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common name: |
barley |
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cultivar: |
Barke |
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lib name: |
IPKc 2026 |
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tissue: |
male inflorescences |
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stage/tissue: |
male inflorescences (approx. 2 mm in size), green anther stage |
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cloning vector: |
pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA) |
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host: |
XL10-Gold |
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antibiotic: |
Ampicillin |
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library description: |
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Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.
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HM14O03r
CTGCGTGCCG GCCGGAGCGG CTTTGCTGTT GTAATCCGCC GTGTTTTCAT TAGTTCTGTA 60
TACGCTGATG ATGTACCATC ATGAGTATGT ATGTATGTAT GTGAGATGGT GAAAGATTGA 120
TCTGGACAAG TAGTGCAACC GCGTGTTTAT TCAGTGCCAC ATGAATGATT TTATCTCTCT 180
CTTAAAAGC 189
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