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Clone: HG01L07

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2023
  tissue: green leaves
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 800 bp

Related with
LOC_Os09g30340.1 annotated gene (TIGR5)
LOC_Os09g30350.1 annotated gene (TIGR5)
Sbi.12403 unigene cluster
Os.12127 unigene cluster
Vvi.6924 unigene cluster
Hv.23072 unigene cluster
Zm.80765 unigene cluster
LOC_Os09g30350.1 annotated gene (MSU 6.1)
LOC_Os09g30340.1 annotated gene (MSU 6.1)
 
Sequence: HG01L07r
submitter of sequence: Dr. Nils Stein
length: 454
priming: 5-prime
GenBankAc: BQ463878
IPK CR-EST: HG01L07r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HG01L07r
CGGCACGAGG CGAGATCAAG GACTCGCGGC GCGTTGGTCC CTGAGCACCC ACCCACCCAC         60
ACAGCTCCAG CTCCGAAGGA GGAGTGAGGA CCATGGCCAC CTCCACCGCC GCCGTGCTGT        120
CCCCGCCCGC CGTGGCCGGG CTCCGCCTAG CGCCGTCCCC GCGCGCGGCG GCCTCGTTCC        180
GGGGGGTCTC ACCGCCGGCG CGGCGGTCCG TGGCGGCGCG GGCCGCGCTG GAGCCGTCGG        240
TGGTGATCAG CCTCAGCACG GGGCTGTCGC TCGTGATGGG CCGCTTCGTC TTCTTCAACT        300
TCCAGCGGGA GAACGTGGCG AAGCAGGTCC CCGAGCAGAA CGGCAAGACC CACTTCGAGG        360
CCGGCGACGA GCGCGCCAAG GAGTTCGCCG GGATCCTCAA GTCCAACGAC CCCGTCGGCT        420
TCAACCTCGT CGACGTCCTC GCATGGGGCT CCAT                                    454
TypePositionOrientation
ORF 1 - 84: forward
ORF 93 - 452: forward
ORF 452 - 192: reverse
ORF 454 - 2: reverse
Cluster Info:
Contig40620.1 (29 members)
view Blast Searches
 
Sequence: HG01L07u
submitter of sequence: Dr. Nils Stein
length: 351
priming: 3-prime
GenBankAc: BQ660035
IPK CR-EST: HG01L07u 
HG01L07u
GTCCAGATCA ATCTTTCACC ATCTCACATA CATACATACA TACTCATGAT GGTACATCAT         60
CAGCGTATAC AGAACTAATG AAAACACGGC GGATTACAAC AGCAAAGCCG CTCCGGCCGG        120
CACGCAGGCA GGCAGGGGAG ACGGACGGAT CAGCCGAAGA AGGGAGGGTC GTATCCGTTG        180
CTGGTGGTGG CGAGGATGTA GTAGGCGACG ATGTGGCCGA TGGAGCCCCA TGCGAGGACG        240
TCGACGAGGT TGAAGCCGAC GGGGTCGTTG GACTTGAGGA TCCCGGCGAA CTCCTTGGCG        300
CGCTCGTCGC CGGCCTCGAA GTGGGTCTTG CCGTTCTGCT CGGGGACCTG C                 351
TypePositionOrientation
ORF 1 - 75: forward
ORF 78 - 350: forward
ORF 211 - 351: forward
ORF 349 - 47: reverse
ORF 350 - 153: reverse
ORF 351 - 97: reverse
Cluster Info:
Contig40620.1 (29 members)
view Blast Searches
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