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Clone: HA25B19

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
Hv.23072 unigene cluster
 
Sequence: HA25B19r
submitter of sequence: Dr. Nils Stein
length: 338
priming: 5-prime
GenBankAc: BU981959
IPK CR-EST: HA25B19r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA25B19r
CGGCACGAGG GCGCGCCAAG GAGTTCGCCG GGATCCTCAA GTCCAACGAC CCCGTCGGCT         60
TCAACCTCGT CGACGTCCTC GCATGGGGCT CCATCGGCCA CATCGTCGCC TACTACATCC        120
TCGCCACCAC CAGCAACGGA TACGACCCTC CCTTCTTCGG CTGATCCGTC CGTCTCCCCT        180
GCCTGCCTGC GTGCCGGCCG GAGCGGCTTT GCTGTTGTAA TCCGCCGTGT TTTCATTAGT        240
TCTGTATACG CTGATGATGT ACCATCATGA GTATGTATGT ATGTATGTGA GATGGTGAAA        300
GATTGATCTG GACAAGTAGT GCAACCGCGT GTTTATTC                                338
TypePositionOrientation
ORF 1 - 267: forward
ORF 2 - 217: forward
ORF 3 - 161: forward
ORF 103 - 2: reverse
ORF 236 - 3: reverse
ORF 336 - 268: reverse
ORF 337 - 239: reverse
Cluster Info:
Contig40620.1 (29 members)
view Blast Searches
 
Sequence: RUS22A02w
submitter of sequence: Dr. Nese Sreenivasulu
length: 293
priming: 3-prime
GenBankAc: FD520994
IPK CR-EST: RUS22A02w 
RUS22A02w
CGGTTGCACT ACTTGTCCAG ATCAATCTTT CACCATCTCA CATACATACA TACATACTCA         60
TGATGGTACA TCATCAGCGT ATACAGAACT AATGAAAACA CGGCGGATTA CAACAGCAAA        120
GCCGCTCCGG CCGGCACGCA GGCAGGCAGG GGAGACGGAC GGATCAGCCG AAGAAGGGAG        180
GGTCGTATCC GTTGCTGGTG GTGGCGAGGA TGTAGTAGGC GACGATGTGG CCGATGGAGC        240
CCCATGCGAG GACGTCGACG AGGTTGAAGC CGACGGGGTC GTTGGACTTG AGG               293
TypePositionOrientation
ORF 3 - 89: forward
ORF 92 - 292: forward
ORF 225 - 293: forward
ORF 291 - 61: reverse
ORF 292 - 167: reverse
ORF 293 - 111: reverse
Cluster Info:
Contig40620.1 (29 members)
view Blast Searches
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