GABI Primary Database ( GabiPD ) - Genomanalyse im biologischen System Pflanze

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Clone: GNW004E12

Genotype (Genotype)

	species:	Hordeum vulgare
	type:	wildtype
	common name:	barley
	cultivar:	Nickel

Library:

	lib name:	IPKc 2051
	tissue:	roots
	stage/tissue:	roots, Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculation) with Fusarium culmorum KF 350
	cloning vector:	pTriplEx2 site_1: Sfi IA; site_2: Sfi IB
	host:	E. coli BM25.8
	antibiotic:	Ampicillin
	library description:
	PolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. (GABI-Agrotech Project)

Related with

LOC_Os01g55830.1	annotated gene (TIGR5)
Hv.932	unigene cluster
Hv.3134	unigene cluster
Zm.81924	unigene cluster
Zm.9	unigene cluster
Sof.20462	unigene cluster
Os.97997	unigene cluster
LOC_Os01g55830.1	annotated gene (MSU 6.1)

Sequence: GNW004E12r

submitter of sequence:	Dr. Sophia Biemelt
length:	588
priming:	5-prime
GenBankAc:	DN158932
IPK CR-EST:	GNW004E12r

Sequence features and Clone alignments (BLAST):

	X

Gene structure	UTR	start codon	stop codon	exon-exon border
Clone alignments	forward	reverse	start	stop
Protein domains	domain

Click on sequence features to obtain additional information.

GNW004E12r
ACGGCTGAAA CAATATCTGG GCACAATTTA TTATCAAACC AGAGCACGGG AATGCTCATT         60
TAAGTACAAG ATTCGACAGG ATCGCAAGCA CAAGCCTGAA CATTCAACAC CAGGACACAT        120
CACAAGAACA CACACTAGGA AAGGCACACA TCGGGGGTGA CACACCTCGG CTTATTTCGA        180
CGAACAAGCC CCAGAAACTA AGATCATGGC TTCATCAGCG CAGCGACCTT CTGGACGGAC        240
GGCCTCGTCA GCAGGTCAGT CCACCAGGCC TTCACATGCG GGTAAGCGTC AAACAGAGAT        300
GCATAGGGTG TGGCCGCCAA GCACAGGGTG ACAGACGCAT GGTTAAGGTC CGCAAGGCTG        360
AGGAAGTCTC CAGCCAGGTA CTTGGACTTG GTGAGCCGCG CCTCGTACAC CGCCAGCACG        420
TTCTTGATCT TAACAAGGTT GTCGTCGATG ACCTTCTGGT CAGTGGCTCC CCCAAGCATA        480
GGATGGATAA GGCACTCCAA GAGAATGGGG CTCAGAGCGG CGGTGTACTG ATGGGCCTCC        540
ACCTCGAGCC ACACATCCAC CATTGCTGAC TCCTTGATGT CGCCCTCC                     588

Type	Position	Orientation
ORF	2 - 157:	forward
ORF	120 - 37:	reverse
ORF	206 - 328:	forward
ORF	276 - 566:	forward
ORF	340 - 275:	reverse
ORF	448 - 528:	forward
ORF	586 - 515:	reverse
ORF	587 - 207:	reverse
ORF	588 - 463:	reverse

Cluster Info:
Contig168.1	(88 members)

view Blast Searches

Oryza sativa pep on 09-MAR-07
E-val	simil.	overlap	accno	description
04e-36	58	240	TIGR:LOC_Os01g27210.1	LOC_Os01g27210.1\|11971.m09172\|protein Glutathione S-transferase IV, putative, expressed
09e-36	57	220	TIGR:LOC_Os01g27340.1	LOC_Os01g27340.1\|11971.m09184\|protein glutathione S-transferase GSTF1, putative, expressed
02e-42	61	219	TIGR:LOC_Os01g27360.1	LOC_Os01g27360.1\|11971.m09186\|protein glutathione S-transferase GSTF1, putative, expressed
02e-40	61	218	TIGR:LOC_Os01g27260.1	LOC_Os01g27260.1\|11971.m09177\|protein glutathione S-transferase GSTF1, putative, expressed
03e-49	70	215	TIGR:LOC_Os01g55830.1	LOC_Os01g55830.1\|11971.m11739\|protein glutathione S-transferase GSTF2, putative, expressed
non redundant GenBank CDS translations \+ PDB \+ SwissProt \+ PIR on 09-MAR-07
E-val	simil.	overlap	accno	description
06e-53	78	214	GenBankAc:CAA29928.1	emb\|CAA29928.1\| unnamed protein product [Zea mays] sp\|P12653\|GSTF1_MAIZE Glutathione S-transferase I (GST-I) (GST-29) (GST class-phi)
06e-53	78	214	GenBankAc:AAA72758.1	gb\|AAA72758.1\| glutathione S-transferase
06e-53	78	213	GenBankAc:1BYE	pdb\|1BYE\|D Chain D, Glutathione S-Transferase I From Mais In Complex With Atrazine Glutathione Conjugate pdb\|1BYE\|C Chain C, Glutathione S-Transferase I From Mais In Complex With Atrazine Glutathione Conjugate pdb\|1BYE\|B Chain B, Glutathione S-Transferase I From Mais In Complex With Atrazine Glutathione Conjugate pdb\|1BYE\|A Chain A, Glutathione S-Transferase I From Mais In Complex With Atrazine Glutathione Conjugate
01e-64	96	212	GenBankAc:CAD29480.1	emb\|CAD29480.1\| glutathione transferase F1 [Triticum aestivum]
09e-64	95	212	GenBankAc:AAK66773.1	gb\|AAK66773.1\| glutathione S-transferase [Triticum aestivum] gb\|AAD56395.1\| glutathione S-transferase [Triticum aestivum]
All nt+env_nt+patnt+pdbnt on 31-MAR-10
E-val	simil.	overlap	accno	description
00e1	99.7	586	GenBankAc:AK249282.1	Hordeum vulgare subsp. vulgare cDNA clone: FLbaf24h17, mRNA sequence
00e1	99.5	586	GenBankAc:AK252867.1	Hordeum vulgare subsp. vulgare cDNA clone: FLbaf173k07, mRNA sequence
00e1	94.3	579	GenBankAc:BT009482.1	Triticum aestivum clone wr1.pk0039.e11:fis, full insert mRNA sequence
00e1	94.3	579	GenBankAc:GP696059.1	Sequence 65684 from patent US 7569389
00e1	94.3	579	GenBankAc:BT009615.1	Triticum aestivum clone wre1n.pk0107.g10:fis, full insert mRNA sequence
00e1	93.6	579	GenBankAc:DQ245588.1	Zea mays clone 17829 mRNA sequence
00e1	93.6	579	GenBankAc:AJ440796.1	Triticum aestivum mRNA for glutathione transferase F1 (gstf1 gene)
00e1	93.6	579	GenBankAc:A98037.1	Sequence 7 from Patent WO9914337
00e1	93.6	579	GenBankAc:AR533628.1	Sequence 7 from patent US 6730828
00e1	93.9	576	GenBankAc:BT009505.1	Triticum aestivum clone wr1.pk0088.e4:fis, full insert mRNA sequence
00e1	94.4	574	GenBankAc:AF184059.1	Triticum aestivum glutathione S-transferase (GST1) mRNA, complete cds
00e1	94.4	574	GenBankAc:GP695824.1	Sequence 65214 from patent US 7569389
01e-179	91.3	574	GenBankAc:EA137478.1	Sequence 1793 from patent US 7214786
00e1	94.4	573	GenBankAc:AF387085.1	Triticum aestivum glutathione S-transferase mRNA, complete cds
00e1	93.7	571	GenBankAc:HB843069.1	Sequence 192283 from Patent EP2090662
00e1	93.7	571	GenBankAc:BT009513.1	Triticum aestivum clone wr1.pk0098.b9:fis, full insert mRNA sequence
00e1	97.3	564	GenBankAc:AJ419775.1	Hordeum vulgare mRNA for glutathione-S-transferase I subunit (gstI gene)
00e1	99.3	552	GenBankAc:DD470508.1	Detection instruments using polynucleotide mapped on barley chromosome
00e1	99.2	385	GenBankAc:FB846039.1	Sequence 65312 from Patent WO2008034648
00e1	99.2	385	GenBankAc:FB871071.1	Sequence 90344 from Patent WO2008034648
00e1	99.2	385	GenBankAc:HB679770.1	Sequence 28984 from Patent EP2090662
00e1	99.2	385	GenBankAc:HB716098.1	Sequence 65312 from Patent EP2090662
00e1	99.2	385	GenBankAc:HB741130.1	Sequence 90344 from Patent EP2090662
00e1	99.2	385	GenBankAc:FB809711.1	Sequence 28984 from Patent WO2008034648
00e1	99	385	GenBankAc:HB843089.1	Sequence 192303 from Patent EP2090662
00e1	96.1	385	GenBankAc:FB809697.1	Sequence 28970 from Patent WO2008034648
00e1	96.1	385	GenBankAc:HB741116.1	Sequence 90330 from Patent EP2090662
00e1	96.1	385	GenBankAc:HB716084.1	Sequence 65298 from Patent EP2090662
00e1	96.1	385	GenBankAc:HB679756.1	Sequence 28970 from Patent EP2090662
00e1	96.1	385	GenBankAc:FB871057.1	Sequence 90330 from Patent WO2008034648
00e1	96.1	385	GenBankAc:FB846025.1	Sequence 65298 from Patent WO2008034648
01e-179	95.8	385	GenBankAc:HB679796.1	Sequence 29010 from Patent EP2090662
01e-179	95.8	385	GenBankAc:FB871097.1	Sequence 90370 from Patent WO2008034648
01e-179	95.8	385	GenBankAc:HB741156.1	Sequence 90370 from Patent EP2090662
01e-179	95.8	385	GenBankAc:HB716124.1	Sequence 65338 from Patent EP2090662
01e-179	95.8	385	GenBankAc:FB809737.1	Sequence 29010 from Patent WO2008034648
01e-179	95.8	385	GenBankAc:FB846065.1	Sequence 65338 from Patent WO2008034648
01e-101	87.5	383	GenBankAc:FP091325.1	Phyllostachys edulis cDNA clone: bphyem105f23, full insert sequence
01e-101	87.5	383	GenBankAc:FP096838.1	Phyllostachys edulis cDNA clone: bphyst027o23, full insert sequence
01e-101	87.5	383	GenBankAc:FP091991.1	Phyllostachys edulis cDNA clone: bphyem112i01, full insert sequence
01e-101	87.5	383	GenBankAc:FP097281.1	Phyllostachys edulis cDNA clone: bphyem118h13, full insert sequence
01e-101	87.5	383	GenBankAc:FP097372.1	Phyllostachys edulis cDNA clone: bphylf024n02, full insert sequence
01e-101	87.5	383	GenBankAc:FP092871.1	Phyllostachys edulis cDNA clone: bphylf056a17, full insert sequence
01e-101	87.5	383	GenBankAc:FP098815.1	Phyllostachys edulis cDNA clone: bphyem124p12, full insert sequence
01e-101	87.5	383	GenBankAc:FP100496.1	Phyllostachys edulis cDNA clone: bphylf035e06, full insert sequence
01e-101	87.5	383	GenBankAc:FP095629.1	Phyllostachys edulis cDNA clone: bphyem205h07, full insert sequence
05e-34	80.1	382	GenBankAc:AY553635.1	Cynodon dactylon glutathione s-transferase II mRNA, complete cds
01e-59	82.9	381	GenBankAc:EU719194.1	Phragmites australis glutathione S-transferase (GST) mRNA, partial cds
08e-39	80.8	380	GenBankAc:CT832486.1	Oryza sativa (indica cultivar-group) cDNA clone:OSIGCEA041B23, full insert sequence
02e-36	80.5	380	GenBankAc:AK099142.1	Oryza sativa Japonica Group cDNA clone:J023055F24, full insert sequence
02e-36	80.5	380	GenBankAc:NM_001050874.1	Oryza sativa (japonica cultivar-group) Os01g0764000 (Os01g0764000) mRNA, complete cds
02e-36	80.5	380	GenBankAc:EF576347.1	Oryza sativa (indica cultivar-group) clone OSS-1-96-F5 glutathione s-transferase ii mRNA, partial cds
02e-36	80.5	380	GenBankAc:AK059818.1	Oryza sativa Japonica Group cDNA clone:006-205-C06, full insert sequence
02e-36	80.5	380	GenBankAc:AP003239.3	Oryza sativa Japonica Group genomic DNA, chromosome 1, PAC clone:P0403C05
02e-36	80.5	380	GenBankAc:AF062403.1	Oryza sativa glutathione S-transferase II mRNA, complete cds
02e-36	80.5	380	GenBankAc:AK058894.1	Oryza sativa Japonica Group cDNA clone:001-007-E12, full insert sequence
01e-34	80.3	380	GenBankAc:EF206786.1	Oryza sativa (indica cultivar-group) glutathione S-transferase (GST) mRNA, partial cds
04e-53	82.7	370	GenBankAc:EU943217.1	Zea mays clone 1587950 mRNA sequence
04e-53	82.7	370	GenBankAc:AY103561.1	Zea mays PCO151099 mRNA sequence
03e-57	82.9	369	GenBankAc:NM_001111942.1	Zea mays glutathione-S-transferase1 (gst1), mRNAemb\|X06754.1\| Maize mRNA for GSH gluthathione S-transferase I (GST;EC 2.5.1.18)
03e-57	82.9	369	GenBankAc:BT009142.1	Triticum aestivum clone wl1n.pk0053.d10:fis, full insert mRNA sequence
03e-57	82.9	369	GenBankAc:BT009155.1	Triticum aestivum clone wl1n.pk0068.a8:fis, full insert mRNA sequence
03e-57	82.9	369	GenBankAc:EU953165.1	Zea mays clone 1387746 glutathione S-transferase GSTF2 mRNA, complete cds
03e-57	82.9	369	GenBankAc:EU954484.1	Zea mays clone 1468575 glutathione S-transferase GSTF2 mRNA, complete cds
03e-57	82.9	369	GenBankAc:EU960197.1	Zea mays clone 222503 glutathione S-transferase GSTF2 mRNA, complete cds
03e-57	82.9	369	GenBankAc:EU964244.1	Zea mays clone 277280 glutathione S-transferase GSTF2 mRNA, complete cds
03e-57	82.9	369	GenBankAc:BT069650.1	Zea mays full-length cDNA clone ZM_BFb0194N18 mRNA, complete cds
03e-57	82.9	369	GenBankAc:EZ065567.1	TSA: Zea mays contig01200, mRNA sequence
03e-57	82.9	369	GenBankAc:FB809657.1	Sequence 28930 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB809665.1	Sequence 28938 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB809809.1	Sequence 29082 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB845985.1	Sequence 65258 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB845993.1	Sequence 65266 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB846137.1	Sequence 65410 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB871017.1	Sequence 90290 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB871025.1	Sequence 90298 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:FB871169.1	Sequence 90442 from Patent WO2008034648
03e-57	82.9	369	GenBankAc:HB679716.1	Sequence 28930 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB679724.1	Sequence 28938 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB679868.1	Sequence 29082 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB716044.1	Sequence 65258 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB716052.1	Sequence 65266 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB716196.1	Sequence 65410 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB741076.1	Sequence 90290 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB741084.1	Sequence 90298 from Patent EP2090662
03e-57	82.9	369	GenBankAc:HB741228.1	Sequence 90442 from Patent EP2090662
03e-57	82.9	369	GenBankAc:GP521774.1	Sequence 8249 from patent US 7572902
03e-57	82.9	369	GenBankAc:GP521836.1	Sequence 8311 from patent US 7572902
03e-57	82.9	369	GenBankAc:GP527437.1	Sequence 13912 from patent US 7572902
03e-57	82.9	369	GenBankAc:GP527513.1	Sequence 13988 from patent US 7572902
03e-57	82.9	369	GenBankAc:GP527529.1	Sequence 14004 from patent US 7572902
03e-57	82.9	369	GenBankAc:GP694401.1	Sequence 62368 from patent US 7569389
06e-55	82.7	369	GenBankAc:BT017983.1	Zea mays clone EL01N0525D04.c mRNA sequence
02e-52	82.7	369	GenBankAc:GP657761.1	Sequence 65765 from patent US 7560542
06e-55	82.7	369	GenBankAc:GP654684.1	Sequence 62688 from patent US 7560542
02e-52	82.4	369	GenBankAc:EU972119.1	Zea mays clone 375445 mRNA sequence
02e-52	82.4	369	GenBankAc:M24889.1	Artificial maize glutathione S-transferase gene, complete cds
04e-53	82.4	369	GenBankAc:M16902.1	Maize glutathione S-transferase gene (GST-I), exons 2 and 3
02e-51	82.1	369	GenBankAc:M16901.1	Maize glutathione S-transferase (GST-I) mRNA, complete cds
02e-48	81.8	369	GenBankAc:GP526102.1	Sequence 12577 from patent US 7572902
03e-38	80.8	369	GenBankAc:XM_002458496.1	Sorghum bicolor hypothetical protein, mRNA
04e-56	82.8	367	GenBankAc:EU971022.1	Zea mays clone 355972 glutathione S-transferase GSTF2 mRNA, complete cds
04e-56	82.8	367	GenBankAc:FB809659.1	Sequence 28932 from Patent WO2008034648
04e-56	82.8	367	GenBankAc:FB845987.1	Sequence 65260 from Patent WO2008034648
04e-56	82.8	367	GenBankAc:FB871019.1	Sequence 90292 from Patent WO2008034648
04e-56	82.8	367	GenBankAc:HB679718.1	Sequence 28932 from Patent EP2090662
04e-56	82.8	367	GenBankAc:HB741078.1	Sequence 90292 from Patent EP2090662
04e-56	82.8	367	GenBankAc:HB716046.1	Sequence 65260 from Patent EP2090662
02e-51	80.7	367	GenBankAc:EA227840.1	Sequence 92155 from patent US 7214786
01e-49	82.4	364	GenBankAc:GP527421.1	Sequence 13896 from patent US 7572902
03e-35	80.5	364	GenBankAc:AY823546.1	Pennisetum glaucum glutathione S-transferase mRNA, complete cds
03e-35	80.5	364	GenBankAc:FB809681.1	Sequence 28954 from Patent WO2008034648
03e-35	80.5	364	GenBankAc:FB846009.1	Sequence 65282 from Patent WO2008034648
03e-35	80.5	364	GenBankAc:FB871041.1	Sequence 90314 from Patent WO2008034648
03e-35	80.5	364	GenBankAc:HB679740.1	Sequence 28954 from Patent EP2090662
03e-35	80.5	364	GenBankAc:HB716068.1	Sequence 65282 from Patent EP2090662
03e-35	80.5	364	GenBankAc:HB741100.1	Sequence 90314 from Patent EP2090662
01e-53	82.6	363	GenBankAc:BT009173.1	Triticum aestivum clone wl1n.pk0107.g3:fis, full insert mRNA sequence
06e-49	82.1	363	GenBankAc:GP526519.1	Sequence 12994 from patent US 7572902
05e-68	84.4	359	GenBankAc:EA152302.1	Sequence 16617 from patent US 7214786
01e-49	82.4	352	GenBankAc:GP516056.1	Sequence 2531 from patent US 7572902
05e-43	81.5	352	GenBankAc:GP657728.1	Sequence 65732 from patent US 7560542
09e-48	82.4	341	GenBankAc:GP518630.1	Sequence 5105 from patent US 7572902
04e-19	79.2	327	GenBankAc:AJ002381.1	Oryza sativa mRNA for second glutathione S-transferase, RGST II
01e-43	82.5	314	GenBankAc:GP669241.1	Sequence 77245 from patent US 7560542
03e-38	81.9	309	GenBankAc:GP652850.1	Sequence 60854 from patent US 7560542
05e-37	81.8	303	GenBankAc:GP530163.1	Sequence 16638 from patent US 7572902
UniProt 15.12 UniRef100 on 28-DEC-09
E-val	simil.	overlap	accno	description
04e-65	98.4	127	UniProt_UniRef:UniRef100_Q8LPD5	Glutathione-S-transferase, I subunit n=1 Tax=Hordeum vulgare RepID=Q8LPD5_HORVU
03e-64	96.1	127	UniProt_UniRef:UniRef100_Q8GTB7	Glutathione transferase F1 n=1 Tax=Triticum aestivum RepID=Q8GTB7_WHEAT
03e-63	95.3	127	UniProt_UniRef:UniRef100_Q9SP56	Glutathione S-transferase n=1 Tax=Triticum aestivum RepID=Q9SP56_WHEAT
02e-52	78.7	127	UniProt_UniRef:UniRef100_P12653	Glutathione S-transferase 1 n=1 Tax=Zea mays RepID=GSTF1_MAIZE
01e-52	78.7	127	UniProt_UniRef:UniRef100_B6U1A7	Glutathione S-transferase GSTF2 n=1 Tax=Zea mays RepID=B6U1A7_MAIZE
02e-52	78.7	127	UniProt_UniRef:UniRef100_B6SKA0	Glutathione S-transferase GSTF2 n=1 Tax=Zea mays RepID=B6SKA0_MAIZE
03e-51	76.4	127	UniProt_UniRef:UniRef100_Q5MJ30	Glutathione S-transferase n=1 Tax=Pennisetum glaucum RepID=Q5MJ30_PENAM
01e-51	76.4	127	UniProt_UniRef:UniRef100_B3GQR4	Glutathione S-transferase (Fragment) n=1 Tax=Phragmites australis RepID=B3GQR4_PHRAU
08e-50	74	127	UniProt_UniRef:UniRef100_Q6Q7Y2	Glutathione s-transferase II n=1 Tax=Cynodon dactylon RepID=Q6Q7Y2_CYNDA
01e-51	77	126	UniProt_UniRef:UniRef100_C5XKV8	Putative uncharacterized protein Sb03g035420 n=1 Tax=Sorghum bicolor RepID=C5XKV8_SORBI
Oryza sativa japonica annotation TIGR 6.1 (cDNA)20091211 on 14-DEC-09
E-val	simil.	overlap	accno	description
05e-39	80.5	380	GabiPD:LOC_Os01g55830.1 TIGR:LOC_Os01g55830.1	LOC_Os01g55830.1\|protein glutathione S-transferase, putative, expressed (TIGR 6.1)
UniGeneDec2009.fasta on 14-DEC-09
E-val	simil.	overlap	accno	description
00e1	99.7	586	GabiPD:Hv.932 unigene:Hv.932	Hv.932
00e1	99.5	586	GabiPD:Hv.3134 unigene:Hv.3134	Hv.3134
00e1	93.6	579	GabiPD:Zm.81924 unigene:Zm.81924	Zm.81924
01e-40	80.8	380	GabiPD:Os.97997 unigene:Os.97997	Os.97997
02e-54	82.4	369	GabiPD:Zm.9 unigene:Zm.9	Zm.9
07e-45	81.3	369	GabiPD:Sof.20462 unigene:Sof.20462	Sof.20462

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