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Clone: GNW001G01

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Nickel
Library:
  lib name: IPKc 2051
  tissue: roots
  stage/tissue: roots, Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degr. C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculation) with Fusarium culmorum KF 350
  cloning vector: pTriplEx2 site_1: Sfi IA; site_2: Sfi IB
  host: E. coli BM25.8
  antibiotic: Ampicillin
  library description:
  PolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. (GABI-Agrotech Project)

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Zm.9 unigene cluster
Sbi.17905 unigene cluster
Sof.20462 unigene cluster
Sbi.14146 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: GNW001G01r
submitter of sequence: Dr. Sophia Biemelt
length: 603
priming: 5-prime
GenBankAc: CX630665
IPK CR-EST: GNW001G01r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
GNW001G01r
GGCAGGTGCC AGCTTTGCAG GATGGTGACT TGTATGTCTT CGAATCACGT GCTATTTGCA         60
AGTATGCGTG CCGCAAGAAC AAGCCAGAGC TGTTGAAGGA GGGCGACATC AAGGAGTCAG        120
CAATGGTGGA TGTGTGGCTC GAGGTGGAGG CCCATCAGTA CACCGCCGCT CTGAGCCCCA        180
TTCTCTTCGA GTGCCTTATC CATCCCATGC TTGGGGGAGC CACTGACCAG AAGGTCATCG        240
ACGACAACCT TGTTAAGATC AAGAACGTGC TGGCGGTGTA CGAGGCGCGG CTCACCAAGT        300
CCAAGTACCT GGCTGGAGAC TTCCTCAGCC TTGCGGACCT TAACCATGCG CCTGTCACCC        360
TGTGCTTGGC GGCCACACCC TATGCATCTC TGTTTGACGC TTACCCGCAT GTGAAGGCCT        420
GGTGGACTGA CCTGCTGACG AGGCCGTCCG TCCAGAAGGT CGCTGCGCTG ATGAAGCCAT        480
GATCTTAGTT TCTGGGGCTT GTTCGTCGAA ATAAGCCGAG GTGTGTCACC CCCGATGTGT        540
GCCTTTCCTA CTGTGTGTTC TTGTGATGTG TCCTGGTGTT GAATGTTCAG GCTTGTGCTT        600
GCC                                                                      603
TypePositionOrientation
ORF 1 - 93: forward
ORF 3 - 479: forward
ORF 109 - 2: reverse
ORF 238 - 158: reverse
ORF 346 - 411: forward
ORF 480 - 358: reverse
ORF 602 - 120: reverse
ORF 603 - 529: reverse
Cluster Info:
Contig168.1 (88 members)
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