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Clone: GCW003P24

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Carola
Library:
  lib name: IPKc 2049
  tissue: roots
  stage/tissue: Seedlings were grown in a mixture of SERAMIS and OIL DRI soil at 20 degree C, 16h light and 50-60% rel. humidity for 7 days, Roots were harvested 6, 24, 48, 72, and 96 hpi (hours post inoculati on) with Fusarium culmorum KF 350
  cloning vector: pTriplEx2 site_1: Sfi IA; site_2: Sfi IB
  host: E. coli BM25.8
  antibiotic: Ampicillin
  library description:
  PolyA-RNA was isolated from water treated roots and used to create a cDNA-library by means of the SMART cDNA library construction Kit (CLONTECH). CDNA Fragments were inserted into the vector Lambda TripleEx Sfi I. Subsequently, plasmids were obtained by in vivo excision according to manufacturers instruction. (GABI-Agrotech Project)

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
Hv.3134 unigene cluster
Hv.932 unigene cluster
Zm.81924 unigene cluster
Zm.9 unigene cluster
Sbi.17905 unigene cluster
Sof.20462 unigene cluster
Sbi.14146 unigene cluster
Os.97997 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: GCW003P24r
submitter of sequence: Dr. Sophia Biemelt
length: 408
priming: 5-prime
GenBankAc: CX629965
IPK CR-EST: GCW003P24r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
GCW003P24r
AGAACGTGCT GTCGGTGTAC GAGGCGCGGC TCACCAAGTC CAAGTACCTG GCTGGAGACT         60
TCCTCAGCCT TGCGGACCTT AACCATGCGT CTGTCACCCT GTGCTTGGCG GCCACACCCT        120
ATGCATCTCT GTTTGACGCT TACCCGCATG TGAAGGCCTG GTGGACTGAC CTGCTGACGA        180
GGCCGTCCGT CCAGAAGGTC GCTGCGCTGA TGAAGCCATG ATCTTAGTTT CTGGGGCTTG        240
TTCGTCGAAA TAAGCCGAGG TGTGTCACCC CCGATGTGTG CCTTTCCTAG TGTGTGTTCT        300
TGTGATGTGT CCTGGTGTTG AATGTTCAGG CTTGTGCTTG CGATCCTGTC GAATCTTGTA        360
CTTAAATGAG CATTCCCGTG CTCTGGTTTG ATAATAAATT GTGCCCGG                     408
TypePositionOrientation
ORF 1 - 150: forward
ORF 2 - 79: forward
ORF 3 - 218: forward
ORF 149 - 3: reverse
ORF 219 - 97: reverse
ORF 305 - 388: forward
ORF 408 - 268: reverse
Cluster Info:
Contig168.1 (88 members)
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