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Clone: MPMGp2010P24110

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: MPMGp2010
  tissue: embryosac
  stage/tissue: 0-10 DAF (days after flowering)
  cloning vector: pQE30NST (AF074376) site_1: SalI; site_2: NotI
  host: E. coli, SCS-1/pSE111
  library description:
  0-10 DAF (days after flowering), cDNA synthesis using pBluescript II XR cDNA-library construction kit (Stratagen) with an oligo(dT)-primer containing NotI restriction site and a SalI adapter (Invitrogen). The main library of 21500 clones was rearrayed into the sublibrary BES 1824 containing 4100 putative expression clones. Note: Due to a cloning artefact caused by the kit, in most cases the SalI site is NOT present, as well as the SalI Adapter used for cloning. To excise the insert, restriction sites upstream SalI should be used (e.g. BamHI). Average insert size is 1 kb. Library generation and sequencing was granted in context of GABI; data are also accessible at https://gabi.rzpd.de

Material available:

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
LOC_Os01g55830.2 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Sbi.17905 unigene cluster
Sbi.17875 unigene cluster
Zm.9 unigene cluster
Sof.20462 unigene cluster
Sbi.18265 unigene cluster
Sbi.14146 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: BES1824110p24
submitter of sequence: Dr. Birgit Kersten
length: 696
tracefile: view (download)
priming: 5-prime
GenBankAc: CK124913
description: For protein expression purposes, please check the reading frame of the Barley coding sequence in relation to the N-terminal His-tag encoded by the vector.
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
BES1824110p24
TCGACCCACG CGTCCGGTCC CATCCCATCC CATCCGAGAA GAAGAGATAG AAAAAAAAAG         60
GTTCGAGGGC GTTGCGGAGT GAGAAGAGAC GAGATGGCTC CGGTGAAGCT GTACGGCGCG        120
ACCCTGTCGT GGAACGTCAC CAGGTGCGTG GCGGCGCTGG AGGAGGCCGG CGTCGAGTAC        180
GAGCTCGTCC CCATCAACTT CAGCACCGGC GAGCACAAGA GCCCCGACCA CCTCGCCAGG        240
AACCCCTTTG GGCAGGTGCC AGCTTTGCAG GATGGTGACT TGTATGTCTT CGAATCACGT        300
GCTATTTGCA AGTATGCGTG CCGCAAGAAC AAGCCAGAGC TGTTGAAGGA GGGCGACATC        360
AAGGAGTCAG CAATGGTGGA TGTGTGGCTC GAGGTGGAGG CCCATCAGTA CACCGCCGCT        420
CTGAGCCCCA TTCTCTTCGA GTGCCTTATC CATCCTATGC TTGGGGGAGC CACTGACCAG        480
AAGGTCATCG ACGACAACCT TGTTAAGATC AAGAACGTGC TGGCGGTGTA CGAGGCGCGG        540
CTCACCAAGT CCAAGTACCT GGCTGGAGAC TTCCTCAGCC TTGCGGACCT TAACCATGCG        600
TCTGTCACCC TGGGCTTGGC GGCCACACCN TATGCATCTC TGTTTGACNC TTACCCGCAT        660
GTGAAGGCCT GGNGGACTGA CNTGCTGACG AGGCCG                                  696
TypePositionOrientation
ORF 2 - 79: forward
ORF 94 - 696: forward
ORF 272 - 343: forward
ORF 359 - 3: reverse
ORF 488 - 408: reverse
ORF 596 - 661: forward
ORF 694 - 608: reverse
ORF 696 - 370: reverse
Cluster Info:
Contig168.1 (88 members)
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