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Clone: MPMGp2010M08109

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: MPMGp2010
  tissue: embryosac
  stage/tissue: 0-10 DAF (days after flowering)
  cloning vector: pQE30NST (AF074376) site_1: SalI; site_2: NotI
  host: E. coli, SCS-1/pSE111
  library description:
  0-10 DAF (days after flowering), cDNA synthesis using pBluescript II XR cDNA-library construction kit (Stratagen) with an oligo(dT)-primer containing NotI restriction site and a SalI adapter (Invitrogen). The main library of 21500 clones was rearrayed into the sublibrary BES 1824 containing 4100 putative expression clones. Note: Due to a cloning artefact caused by the kit, in most cases the SalI site is NOT present, as well as the SalI Adapter used for cloning. To excise the insert, restriction sites upstream SalI should be used (e.g. BamHI). Average insert size is 1 kb. Library generation and sequencing was granted in context of GABI; data are also accessible at https://gabi.rzpd.de

Material available:

Related with
Hv.23678 unigene cluster
Hv.13426 unigene cluster
Hv.8009 unigene cluster
 
Sequence: BES1824109m08
submitter of sequence: Dr. Birgit Kersten
length: 534
tracefile: view (download)
priming: 5-prime
GenBankAc: CK124610
description: For protein expression purposes, please check the reading frame of the Barley coding sequence in relation to the N-terminal His-tag encoded by the vector.
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
BES1824109m08
CTCGTGCGTC CGGGTTTATA CGGACCTCCA TCCCCAAGCC CCCTCCACCA CCCGGCCGCT         60
CCTTCCTCCG GATCGCTCGC CATGTTAAAC GCCCTGGTTA CGTCCATGTT CGCCACGGGT        120
ACGGTGGACG AATATTTCCA GTACCTGCAG TCGATGGACG AGGATGGCAG CTCAGGCCCG        180
GGTTTGGTCA CCGAGGTCAT AAACCTCTTC ATCGGCAACG CCAACGGGAT CCTCAACGAC        240
ATCGCCGGCC TGCTGAACGA GCCCGTGCCA GACTTCGACA AGGTGGACGA CCTGGTGCAT        300
CGGCTCAAGG GCTGCAGCTA CAGTGTCGGT GCGAAGAAAG TGAACCTCTC CTGCATGCAC        360
TTCAACCAGT ACATCGAGGC AAGAAGCAAA GAAGGGTGCC TCATGGCACT GGCTCTTCTT        420
AGGAATGAGT TTTGCGATGT GTCCAACAAG TTCCAGACAA TGATGCAGCT GGAGCAACAG        480
ATCGCGGCCT ATGGTCCTAA TTAGTAGCAG TCAAAGCAAA AAAAAAAAAA AAGG              534
TypePositionOrientation
ORF 1 - 501: forward
ORF 2 - 85: forward
ORF 3 - 419: forward
ORF 242 - 3: reverse
ORF 300 - 175: reverse
ORF 404 - 321: reverse
Cluster Info:
Contig104.4 (2 members)
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