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Clone: MPMGp2010D01106

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: MPMGp2010
  tissue: embryosac
  stage/tissue: 0-10 DAF (days after flowering)
  cloning vector: pQE30NST (AF074376) site_1: SalI; site_2: NotI
  host: E. coli, SCS-1/pSE111
  library description:
  0-10 DAF (days after flowering), cDNA synthesis using pBluescript II XR cDNA-library construction kit (Stratagen) with an oligo(dT)-primer containing NotI restriction site and a SalI adapter (Invitrogen). The main library of 21500 clones was rearrayed into the sublibrary BES 1824 containing 4100 putative expression clones. Note: Due to a cloning artefact caused by the kit, in most cases the SalI site is NOT present, as well as the SalI Adapter used for cloning. To excise the insert, restriction sites upstream SalI should be used (e.g. BamHI). Average insert size is 1 kb. Library generation and sequencing was granted in context of GABI; data are also accessible at https://gabi.rzpd.de

Material available:

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
Hv.22922 unigene cluster
Os.11487 unigene cluster
Sbi.19782 unigene cluster
Sof.5084 unigene cluster
Os.8864 unigene cluster
Zm.138249 unigene cluster
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
 
Sequence: BES1824106d01
submitter of sequence: Dr. Birgit Kersten
length: 199
tracefile: view (download)
priming: 5-prime
GenBankAc: CK122846
description: For protein expression purposes, please check the reading frame of the Barley coding sequence in relation to the N-terminal His-tag encoded by the vector. 
BES1824106d01
CCCTCCCCCC TCCCTGCATC GTCGCGGAAC AGTGCTCCCA CCGCCGGCGC CATGAGTAAG         60
TTGCAGAGCG ATGCCCTGAG GGAGGCTATC ACAAACATTA CCAATGACTG CCGTGAGAAG        120
AACCGCAAGT TCGTTGAAAC CATTGAGCTG CAGATTGGTC TGAAGAACTA TGACCCGCAA        180
AAGGACAAAC GTTTCAGTG                                                     199
TypePositionOrientation
ORF 52 - 198: forward
ORF 143 - 3: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
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