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Clone: HO11C24

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Ingrid BC mlo-5
Library:
  lib name: IPKc 2027
  tissue: leaf epidermis, 6 h and 24 h post inoculation with Blumeria graminis
  stage/tissue: 7 d after germination
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Approximately 5 % of the clones correspond to cDNA from the fungi B. graminis hordei and tritici, respectively. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1.2 kb

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
LOC_Os01g55830.2 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Sbi.17905 unigene cluster
Sbi.17875 unigene cluster
Zm.9 unigene cluster
Sof.19777 unigene cluster
Sof.17942 unigene cluster
Sof.20462 unigene cluster
Sbi.18265 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HO11C24S
submitter of sequence: Dr. Patrick Schweizer
length: 593
priming: 5-prime
GenBankAc: CD056100
IPK CR-EST: HO11C24S
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HO11C24S
CGGCACGAGG AGAAGAAGAG ATAGAAAAAA AAGGTTCGAG GGCGTTGCGG AGTGAGAAGA         60
GACGAGATGG CTCCGGTGAA GCTGTACGGC GCGACCCTGT CGTGGAACGT CACCAGGTGC        120
GTGGCGGCGC TTGAGGAGGC CGGCGTCGAG TACGAGCTCG TCCCCATCAA CTTCGGCACC        180
GGCGAGCACA AGAGCCCCGA CCACCTCGCC AGGAACCCCT TTGGGCAGGT GCCAGCTTTG        240
CAGGATGGTG ACTTGTACGT CTTCGAATCA CGTGCTATTT GCAAGTATGC GTGCCGCAAG        300
AACAAGCCAG AGCTGTTGAA GGAGGGTGAC ATCAAGGAGT CAGCAATGGT GGATGTGTGG        360
CTCGAGGTGG AGGCCCATCA GTACACCGCC GCTCTGAGCC CCATTCTCTT CGAGTGCCTT        420
ATCCATCCTA TGCTTGGGGG AGCCACTGAC CAGAAGGTCA TCGACGACAA CCTTGTTAAG        480
ATCAAGAATG TGCTGGCGGT GTACGAGGCG CGGCTCACCA AGTCCAAGTA CCTGGCTGGA        540
GACTTCCTCA GCCTTGCGGA CCTTAACCAT GCGTCTGTCA CCCTGTGCTT GGC               593
TypePositionOrientation
ORF 3 - 131: forward
ORF 67 - 591: forward
ORF 245 - 316: forward
ORF 332 - 3: reverse
ORF 488 - 592: forward
ORF 591 - 343: reverse
ORF 593 - 381: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
 
Sequence: HO11C24w
submitter of sequence: Dr. Patrick Schweizer
length: 363
priming: 3-prime
GenBankAc: CK568790
IPK CR-EST: HO11C24w 
HO11C24w
CCTGAACATT CTACACCAGG ACACCTCACA AGAACACACA CTACGAAAGG CACACATCGG         60
GGGTGACACA CCTCGGCTTA TTTCGACTAA CAAGCCCCAC AAACCACGAA CATGGTTTCC        120
TCAGCGCCCC CACCTTCTGG ACGGACGGCC TCGTCAGCCG GTCAGTCCAC CAGGCCTTCC        180
CATGCGGGAA AGGGTCGAAC AAAGATGCAT AAGGTGTGGC CGCCAAGCCC AACGAGACAG        240
ACGCATGGTT AAGGTCCCCA AGGCTGAGGA AGTCTCCAGC CACCTACTTG GACTTGGTGA        300
ACCGCGCCTC CTACACCTCC AACACATTCT TGATCTTCAC AAGGTTGACG TCGATGACCT        360
TCT                                                                      363
TypePositionOrientation
ORF 1 - 63: forward
ORF 112 - 249: forward
ORF 182 - 361: forward
ORF 327 - 157: reverse
ORF 362 - 252: reverse
Singlet Info:
AA tgicl singlet
Representative for Uniset
view Blast Searches
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