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Clone: MPIZp769M163Q

Genotype (Genotype)
  species: Arabidopsis thaliana
  type: wildtype
  common name: thale cress
  cultivar: Landsberg erecta (Ler)
Library:
  lib name: MPIZp769
  tissue: whole plant
  stage/tissue: adult plant, mixed stresses
  cloning vector: pSPORT1 site_1: SalI; site_2: NotI
  host: E. coli XL1-Blue MRF
  library description:
  cDNA library from Arabidopsis thaliana, accession Landsberg erecta; six weeks old total plants grown under long-day conditions in soil, whole adult plants were treated for 24 hours with different stresses, (1) at 4? C in the dark, (2), at 37 Grad C in the dark, (3) lying in the lab after removing from soil, (4) in the greenhouse after wounding leaves with a foreceps, (5) in the lab watering with a 150 mM NaCl solution, (6) at 26 Grad C in the light/UV; equal quantities of stressed plant material were pooled; library was made at the Max-Planck-Institute for Plant Breeding Research, Cologne, Germany; cloning sites SalI-NotI, primer sites and orientation: T7-SalI-CCACGCGTCCG-5prime-cDNA-polyA-CC-NotI-SP6; Note: Sequencing granted in the context of the GABI Arabidopsis Verbund I: Genetic Diversity, "Establishment of high-efficiency SNP-based mapping tools and development of methods for genome-wide mutation detection" PI: Bernd Weisshaar Sequence submission managed by RZPD/GABI-Primary database: http://gabi.rzpd.de. This clone is available from RZPD; contact RZPD (clone@rzpd.de) for further information.

Related with
266210_at Affymetrix representative on ATH1-121501
AT2G27530.1 annotated gene (TAIR7)
AT2G27530.2 annotated gene (TAIR7)
AT1G08360.1 annotated gene (TAIR7)
AT5G22440.2 annotated gene (TAIR7)
AT5G22440.1 annotated gene (TAIR7)
At.19006 unigene cluster
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Bra.1910 unigene cluster
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At.24240 unigene cluster
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Csi.5735 unigene cluster
Ccl.2011 unigene cluster
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Sof.5084 unigene cluster
Ghi.18764 unigene cluster
Lsa.11335 unigene cluster
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Nta.6843 unigene cluster
Mtr.2748 unigene cluster
Vvi.9214 unigene cluster
Lsa.14576 unigene cluster
Ghi.4846 unigene cluster
Hv.5459 unigene cluster
Sbi.19782 unigene cluster
Mtr.7684 unigene cluster
Han.5727 unigene cluster
Ptp.5768 unigene cluster
Pth.16459 unigene cluster
Lja.14656 unigene cluster
Lja.541 unigene cluster
Han.9639 unigene cluster
Bna.2003 unigene cluster
Pth.5660 unigene cluster
Ptp.11065 unigene cluster
Lsa.5490 unigene cluster
Ppa.3917 unigene cluster
Gma.6491 unigene cluster
Mtr.17728 unigene cluster
Mtr.16898 unigene cluster
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Ppe.20380 unigene cluster
Han.4573 unigene cluster
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Han.1824 unigene cluster
Gma.37744 unigene cluster
Pta.2091 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Vvi.7697 unigene cluster
AT2G27530.1 annotated gene (TAIR9)
AT2G27530.2 annotated gene (TAIR9)
AT1G08360.1 annotated gene (TAIR9)
AT5G22440.1 annotated gene (TAIR9)
AT5G22440.2 annotated gene (TAIR9)
At.19006 unigene cluster
At2g27530 annotated gene (TIGR5)
 
Sequence: 31-E9518-012-003-M16-T7R
submitter of sequence: Prof. Dr. Bernd Weisshaar
length: 620
priming: 5-prime
GenBankAc: CB261106 
31-E9518-012-003-M16-T7R
ATTCAGGATC AGAGATTCGT GACCTGAGAA GACACTCAAT ATGAGTAAGC TTCAGAGTGA         60
GGCTGTTAGA GAAGCCATAA CGACTATCAA GGGAAAATCT GAGGAGAAGA AGCGTAACTT        120
TGTTGAGACA GTGGAGCTTC AGATTGGTCT GAAGAACTAT GACCCACAAA AGGATAAGCG        180
TTTCAGTGGA TCTGTGAAGC TCCCACATAT TCCTCGCCCA AAAATGAAGA TCTGTATGCT        240
TGGAGATGCT CAGCATGTTG AGGAGGCAGA GAAAATGGGA CTTAGTAACA TGGATGTTGA        300
GGCTTTGAAA AAGCTTAACA AGAACAAGAA GCTTGTTAAG AAGCTTGCTA AGTCTTACCA        360
TGCTTTCTTG GCTTCTGAGT CTGTCATTAA GCAGATTCCT CGTCTTCTTG GTCCTGGTCT        420
TAACAAGGCA GGAAAGTTCC CAACACTTGT GAGTCACCAA GAGTCATTGG AAGCAAAGGT        480
GAATGAGACC AAAGCAACAG TGAAGTTCCA GTTGAAGAAA GTTCTGTGTA TGGGTGTTGC        540
TGTTGGTAAC CTTTCCATGG AAGAGAAGCA GCTCTTCCAG AATGTGCAGA TGAGTGTTAA        600
CTTCCTCGTC TCGCTCTTGA                                                    620
TypePositionOrientation
ORF 41 - 619: forward
ORF 360 - 449: forward
ORF 361 - 2: reverse
ORF 618 - 457: reverse
ORF 619 - 467: reverse
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