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Clone: HC13H03

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Sloop
Library:
  lib name: IPKc 2036
  tissue: coleoptile
  stage/tissue: coleoptile, 3 days old
  cloning vector: pSPORT site_1: SalI (5-end of cDNA); site_2: NotI (3-end of cDNA)
  host: DH10B
  antibiotic: Ampicillin
  library description:
  Due to the cloning system used blue/white selection for recombinants is not 100 % reliable. Average insert size is 1.3 kb.

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
LOC_Os01g55830.2 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Zm.9 unigene cluster
Sof.20462 unigene cluster
Sbi.17905 unigene cluster
Sbi.14146 unigene cluster
Os.97997 unigene cluster
Sof.17942 unigene cluster
Sbi.17875 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HC13H03w
submitter of sequence: Dr. Nils Stein
length: 556
priming: 5-prime
GenBankAc: CB870131
IPK CR-EST: HC13H03w
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HC13H03w
CACGCGTCCG CATCCGAGAA GAAGAGATAG AAAAAAAAAG GTTCGAGGGC GTTGCGGAGT         60
GAGAAGAGAC GAGATGGCTC CGGTGAAGCT GTACGGCGCG ACCCTGTCGT GGAACGTCAC        120
CAGGTGCGTG GCGGCGCTGG AGGAGGCCGG CGTCGAGTAC GAGCTCGTCC CCATCAACTT        180
CAGCACCGGC GAGCACAAGA GCCCCGACCA CCTCGCCAGG AACCCCTTTG GGCAGGTGCC        240
AGCTTTGCAG GATGGTGACT TGTATGTCTT CGAATCACGT GCTATTTGCA AGTATGCGTG        300
CCGCAAGATC AAGCCAGAGC TGTTGAAGGA GGGCGACATC AAGGAGTCAG CAATGGTGGA        360
TGTGTGGCTC GAGGTGGAGG CCCATCAGTA CACCGCCGCT CTGAGCCCCA TTCTCTTCGA        420
GTGCCTTATC CATCCTATGC TTGGGGGAGC CACTGACCAG AAGGTCATCG ACGACAACCT        480
TGTTAAGATC AAGAACGTGC TGGCGGTGTA CGAGGCGCGG CTCACCAAGT CCAAGTACCT        540
GGCTGGAGAC TTCCTC                                                        556
TypePositionOrientation
ORF 74 - 556: forward
ORF 252 - 323: forward
ORF 339 - 1: reverse
ORF 555 - 388: reverse
ORF 556 - 350: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
 
Sequence: HC13H03y
submitter of sequence: Dr. Nils Stein
length: 601
priming: 3-prime
GenBankAc: CB873747
IPK CR-EST: HC13H03y
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HC13H03y
AAAGCAACAG ATGAGAAAAT ATTGAGGCTG AGACAATATC TGGGCACAAT TTATTATCAA         60
ACCAGAGCAC GGGAATGCTC ATTTAAGTAC AAGATTCGAC AGGATCGCAA GCACAAGCCT        120
GAACATTCAA CACCAGGACA CATCACAAGA ACACACACTA GGAAAGGCAC ACATCGGGGG        180
TGACACACCT CGGCTTATTT CGACGAACAA GCCCCAGAAA CTAAGATCAT GGCTTCATCA        240
GCGCAGCGAC CTTCTGGACG GACGGCCTCG TCAGCAGGTC AGTCCACCAG GCCTTCACAT        300
GCGGGTAAGC GTCAAACAGA GATGCATAGG GTGTGGCCGC CAAGCACAGG GTGACAGACG        360
CATGGTTAAG GTCCGCAAGG CTGAGGAAGT CTCCAGCCAG GTACTTGGAC TTGGTGAGCC        420
GCGCCTCGTA CACCGCCAGC ACGTTCTTGA TCTTAACAAG GTTGTCGTCG ATGACCTTCT        480
GGTCAGTGGC TCCCCCAAGC ATAGGATGGA TAAGGCACTC GAAGAGAATG GGGCTCAGAG        540
CGGCGGTGTA CTGATGGGCC TCCACCTCGA GCCACACATC CACCATTGCT GACTCCTTGA        600
T                                                                        601
TypePositionOrientation
ORF 1 - 180: forward
ORF 82 - 2: reverse
ORF 143 - 60: reverse
ORF 229 - 351: forward
ORF 299 - 589: forward
ORF 363 - 298: reverse
ORF 471 - 551: forward
ORF 599 - 486: reverse
ORF 600 - 538: reverse
ORF 601 - 230: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
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