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Clone: HP07N11

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2028
  tissue: epidermis
  stage/tissue: seedlings grown in greenhouse for 6 days
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 850 bp

Related with
Hv.23072 unigene cluster
 
Sequence: HP07N11T
submitter of sequence: Dr. Nils Stein
length: 653
priming: 5-prime
GenBankAc: CB878246
IPK CR-EST: HP07N11T
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HP07N11T
GGGTTGGTCC CTGTAGCACC CACCCACCCA CACAGCTCCA GCTCCGAAGG AGGAGTGAGG         60
ACCATGGCCA CCTCCACCGC CGCCGTGCTG TCCCCGCCCG CCGTGGCCGG GCTCCGCCTA        120
GCGCCGTCCC CGCGCGCGGC GGCCTCGTTC CGGGGGGTCT CACCGCCGGC GCGGCGGTCC        180
GTGGCGGCGC GGGCCGCGCT GGAGCCGTCG GTGGTGATCA GCCTCAGCAC GGGGCTGTCG        240
CTCGTGATGG GCCGCTTCGT CTTCTTCAAC TTCCAGCGGG AGAACGTGGC GAAGCAGGTC        300
CCCGAGCAGA ACGGCAAGAC CCACTTCGAG GCCGGCGACG AGCGCGCCAA GGAGTTCGCC        360
GGGATCCTCA AGTCCAACGA CCCCGTCGGC TTCAACCTCG TCGACGTCCT CGCATGGGGC        420
TCCATCGGCC ACATCGTCGC CTACTACATC CTCGCCACCA CCAGCAACGG ATACGACCCT        480
CCCTTCTTCG GCTGATCCGT CCGTCTCCCC TGCCTGCCTG CGTGCCGGCC GGAGCGGCTT        540
TGCTGTTGTA ATCCGCCGTG TNTTCATTAG TTCTGTATAC GCTGATGATG TACCATCATG        600
AGTATGTATG TATGTATGTG AGATGGTGAA AGATTGATCT GGACAAGTAG TGC               653
TypePositionOrientation
ORF 1 - 492: forward
ORF 3 - 548: forward
ORF 434 - 3: reverse
ORF 651 - 163: reverse
ORF 653 - 570: reverse
Cluster Info:
Contig40620.1 (29 members)
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