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Clone: HH03L18

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Sloop
Library:
  lib name: IPKc 2037
  tissue: coleoptile
  stage/tissue: coleoptile, 1 day old
  cloning vector: pSPORT site_1: SalI (5-end of cDNA); site_2: NotI (3-end of cDNA)
  host: DH10B
  antibiotic: Ampicillin
  library description:
  Due to the cloning system used blue/white selection for recombinants is not 100 % reliable. Average insert size is 1.3 kb.

Related with
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
Nta.1653 unigene cluster
Bra.1732 unigene cluster
Bna.16698 unigene cluster
Bna.15209 unigene cluster
Bol.5797 unigene cluster
Bol.1747 unigene cluster
Vvi.7697 unigene cluster
At.19006 unigene cluster
Ghi.18764 unigene cluster
Bra.28765 unigene cluster
At.24240 unigene cluster
Ghi.5769 unigene cluster
Tcc.1562 unigene cluster
Sof.5084 unigene cluster
Hv.5459 unigene cluster
Zm.66965 unigene cluster
Sbi.837 unigene cluster
Lja.541 unigene cluster
Lsa.5490 unigene cluster
Hv.22922 unigene cluster
Les.19407 unigene cluster
Can.679 unigene cluster
Os.8864 unigene cluster
Nta.6843 unigene cluster
Zm.138249 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bna.4011 unigene cluster
Les.3112 unigene cluster
Can.3824 unigene cluster
Nta.381 unigene cluster
Les.19882 unigene cluster
Can.1546 unigene cluster
Stu.2362 unigene cluster
Bol.3778 unigene cluster
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
 
Sequence: HH03L18u
submitter of sequence: Dr. Nils Stein
length: 357
priming: 3-prime
GenBankAc: CB862610
IPK CR-EST: HH03L18u 
HH03L18u
GTCATGAAGT TCCAGACAAT AGAACAAACC ATCTCAGTAC AAGACAATAA TTGTCACTCG         60
ACGCGCACGA AAATTTGATA ATTGAGAAAG CAACAAAAGT TCCCAAGTTG TCAATCNCCT        120
GGAAATCCTA NAAGACTAGA GTCTAGAACA ACCGGTACAC CTTTCCCATT GNGCTCTTCA        180
GGTACAGGCA CCGCACGTTT TGCCAATTCT TCTTCAACAG AGAGACAAGG AAGTTCACGC        240
TCATCTGCAC GTTCTGGAAG ATCTGCTTCT CCTCCATACC CAGGTGGCCA ACGGNAACAC        300
CCATGCAAAG CACCTTTTTC AACTGGAACT TGACAGTGGC CTTGGTCTCA TTAACCT           357
TypePositionOrientation
ORF 1 - 75: forward
ORF 355 - 146: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
 
Sequence: HH03L18w
submitter of sequence: Dr. Nils Stein
length: 363
priming: 5-prime
GenBankAc: CB860993
IPK CR-EST: HH03L18w 
HH03L18w
CACGCGTCCG CAAGGTTAAT GAGACCAGGC CACTGTCAAG TTCCAGTTGA AAAAGGTGCT         60
TTGCATGGGG TTGCCGTTGG CCACCTGGGT ATGGAGGAGA AGCAGATCTT CCAGAACGTG        120
CAGATGAGCG TGAACTTCTT GTCTCTCTGT TGAAGAAGAA TTGGCAAAAC GTGCGGTGCC        180
TGTACCTGAA GAGCACAATG GGAAAGGGTA CCGGTTGTTC TAGACTCTAG TCTTCTAGGA        240
TTTCCAGGAG ATTGACAACT TGGGAACTTT TGTTGCTTCT CAATTATCAA ATTTTCGTGC        300
GCGTCGAGTG ACAATTATTG TCTTGTACTG AGATGGTTTG TTCTATGTCT GGAACTTCAT        360
GAC                                                                      363
TypePositionOrientation
ORF 1 - 150: forward
ORF 361 - 290: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
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