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Clone: HZ52G19

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2035
  tissue: pericarp
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  pericarp 0-7 DAP(days after pollination). Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 900 bp

Related with
LOC_Os01g55830.2 annotated gene (TIGR5)
LOC_Os01g55830.1 annotated gene (TIGR5)
Hv.3134 unigene cluster
Hv.932 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Sbi.17905 unigene cluster
Sof.17942 unigene cluster
Sbi.17875 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HZ52G19r
submitter of sequence: Dr. Nils Stein
length: 407
priming: 5-prime
GenBankAc: CA025530
IPK CR-EST: HZ52G19r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
HZ52G19r
CGGCACGAGG GTCCCATCCC ATCCCATCCG AGAAGAAGAG ATAGAAAAAA AAAGGTTCGA         60
GGGCGTTGCG GAGTGAGAAG AGACGAGATG GCTCCGGTGA AGCTGTACGG CGCGACCCTG        120
TCGTGGAACG TCACCAGGTG CGTGGCGGCG CTGGAGGAGG CCGGCGTCGA GTACGAGCTC        180
GTCCCCATCA ACTTCAGCAC CGGCGAGCAC AAGAGCCCCG ACCACCTCGC CAGGAACCCC        240
TTTGGGCAGG TGCCAGCTTT GCAGGATGGT GACTTGTATG TCTTCGAATC ACGTGCTATT        300
TGCAAGTATG CGTGCCGCAA GAACAAGCCA GAGCTGTTGA AGGAGGGCGA CATCAAGGAG        360
TCAGCAATGG TGGATGTGTG GCTCGAGGTG GAGGCCCATC AGTACAC                      407
	      
TypePositionOrientation
ORF 2 - 73: forward
ORF 88 - 405: forward
ORF 266 - 337: forward
ORF 353 - 3: reverse
Cluster Info:
Contig168.1 (88 members)
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