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Clone: HX12M02

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2034
  tissue: apex
  stage/tissue: apex (3-5 mm in size)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
Hv.22922 unigene cluster
Os.8864 unigene cluster
Os.11487 unigene cluster
Zm.138249 unigene cluster
Sof.5084 unigene cluster
Sbi.19782 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Sbi.837 unigene cluster
Zm.66965 unigene cluster
Hv.12839 unigene cluster
Bol.1747 unigene cluster
Zm.94920 unigene cluster
Bol.5797 unigene cluster
Hv.5459 unigene cluster
Sof.20573 unigene cluster
At.24240 unigene cluster
Bra.1732 unigene cluster
Vvi.9214 unigene cluster
Les.19407 unigene cluster
Bna.4011 unigene cluster
Bol.3778 unigene cluster
Os.37672 unigene cluster
Tcc.1562 unigene cluster
At.19006 unigene cluster
Can.3824 unigene cluster
Pth.5660 unigene cluster
Ptp.5768 unigene cluster
Nta.6843 unigene cluster
Nta.1653 unigene cluster
Can.679 unigene cluster
Bna.575 unigene cluster
Ccl.2011 unigene cluster
Csi.5735 unigene cluster
Tcc.7897 unigene cluster
Stu.2362 unigene cluster
Pth.17313 unigene cluster
Les.19882 unigene cluster
Ptp.11065 unigene cluster
Les.3112 unigene cluster
Ghi.5769 unigene cluster
Bna.15209 unigene cluster
Psi.8134 unigene cluster
Pgl.4932 unigene cluster
Lja.541 unigene cluster
Ghi.18764 unigene cluster
Bra.28765 unigene cluster
At.7367 unigene cluster
Gma.6491 unigene cluster
Pta.7598 unigene cluster
Pta.24201 unigene cluster
Mtr.2748 unigene cluster
Lsa.5490 unigene cluster
Ppe.5428 unigene cluster
Ppe.20380 unigene cluster
Lja.14656 unigene cluster
Les.23394 unigene cluster
Ppa.3640 unigene cluster
Gma.37744 unigene cluster
Can.1546 unigene cluster
Pta.2091 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Ghi.4846 unigene cluster
Tcc.3944 unigene cluster
Ptp.634 unigene cluster
Pth.16459 unigene cluster
Lsa.11335 unigene cluster
Pth.16449 unigene cluster
Han.9639 unigene cluster
Ppa.5371 unigene cluster
Lsa.14576 unigene cluster
Han.4573 unigene cluster
Ppa.3917 unigene cluster
Han.1824 unigene cluster
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
 
Sequence: HX12M02r
submitter of sequence: Dr. Nils Stein
length: 623
priming: 5-prime
GenBankAc: CA032348
IPK CR-EST: HX12M02r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX12M02r
GTTCGTTGAA ACCTTGAGCT GCAGATTGGT CTGAAGAACT ATGACCCGCA AAAGGACAAA         60
CGTTTCAGTG GCTCTGTTAA GCTTCCTCAC ATCCCTCGCC CAAAGATGAG GGTTTGCATG        120
CTTGGTGATG CCCAGCATGT TGAAGAGGCA GGGAAAATGG GCCTTGACTG TATGGATGTG        180
GAATCGCTGA AGAAGATGAA CAAAAACAAG AAGCTAGTCA AGAAGCTTGC AAAGAAGTAT        240
CATGCTTTCC TTGCATCTGA GGCTATCATT AAGCAGATTC CCCGACTTCT TGGACCTGGT        300
CTTAACAAGG CAGGAAAGTT TCCAACTTTG GTGTCTCACC AGGAGTCCCT TGAGTCCAAG        360
GTTAATGAGA CCAAGGCCAC TGTCAAGTTC CAGTTGAAAA AGGTGCTTTG CATGGGTGTT        420
GCCGTTGGCC ACCTGGGTAT GGAGGAGAAG CAGATCTTCC AGAACGTGCA GATGAGCGTG        480
AACTTCCTTG TCTCTCTGTT GAAGAAGAAT TGGCAAAACG TGCGGTGCCT GTACCTGAAG        540
AGCACAATGG GAAAGGTGTA CCGGTTGTTC TAGACTCTAG TCTTCTAGGA TTTCCAGGAG        600
ATTGACAACT TGGGAACTTT TGT                                                623
TypePositionOrientation
ORF 41 - 106: forward
ORF 106 - 570: forward
ORF 138 - 1: reverse
ORF 242 - 394: forward
ORF 413 - 339: reverse
ORF 622 - 386: reverse
Cluster Info:
Contig155.1 (81 members)
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