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Clone: HX11A08

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2034
  tissue: apex
  stage/tissue: apex (3-5 mm in size)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os03g15590.1 annotated gene (TIGR5)
Hv.2956 unigene cluster
Os.22416 unigene cluster
Sbi.6305 unigene cluster
Zm.2999 unigene cluster
Sof.18024 unigene cluster
Pth.5602 unigene cluster
Mtr.2251 unigene cluster
Ghi.12746 unigene cluster
LOC_Os03g15590.1 annotated gene (MSU 6.1)
 
Sequence: HX11A08r
submitter of sequence: Dr. Nils Stein
length: 527
priming: 5-prime
GenBankAc: CA031748
IPK CR-EST: HX11A08r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX11A08r
CGGCAGAGGG CCGCACGAGG GGGCGCCATG GAGGCGGCGG TGGAGAGGAA GGTGCCCGAG         60
CAGGAGGAGC AGCTACAGCC GTCGCACGCC CGGGCCGAGG ACGCGCCGCC CGCCGCCGTC        120
GAGGAGGAGG ACGAGGCGGA GGCCGAGGAG AGCGAGCGCC GCAACCGCGA GCTCAAGGCC        180
GGCCTCCATC CTCTCAGGCG CAAGCTCGTG CTCTGGTACA CACGCCGGAC GCCCGGGACG        240
AGGTCGCAGT CGTACGAGGA CAACATCAAG AAGATCGTCG ATTTCAGCAC TGTCGAGTCG        300
TTCTGGGTTT GCTACTGCCA CCTCGCACGC CCGTCCGCCC TACCGAGCCC CACGGACCTG        360
CATCTTTTCA AAGAGGGTGT CCGTCCACTC TGGGAGGATC CTGCAAATCG GAATGGCGGC        420
AAGTGGATTA TACGGTTCAA GAAGGCTGTT TCTGGGCGTT TCTGGGAGGA TTTGGTGCTG        480
GTTCTAGTGG GTGACCAACT TGATTATAGT GATGATGTTT GTGGCAT                      527
TypePositionOrientation
ORF 2 - 484: forward
ORF 3 - 491: forward
ORF 28 - 525: forward
ORF 189 - 76: reverse
ORF 266 - 3: reverse
ORF 526 - 440: reverse
Cluster Info:
Contig2204.1 (5 members)
view Blast Searches
 
Sequence: HX11A08w
submitter of sequence: Dr. Nils Stein
length: 553
priming: 3-prime
GenBankAc: CB876351
IPK CR-EST: HX11A08w
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX11A08w
AAAAGTCAAT ACTTAGGCAT TTGAAATTCT GTAGAGTACA TGGTAGCATG ACAAAGAGTA         60
ATCATACATC ATGGCTGGGG TTGAAACTTC AGAACCGAAA ATCCACAAGG ATTAAGTTCA        120
CGAGAAGGGC CATGACTAGC CTTGACAACA TGGAGGTTAA CCTCTCAGCC ATGTGTTCCT        180
GTAGGACGAG TTGTCTCGCA GCGAGGCATC ATGTGGTTTG TACTCCATCA GATAGGTGTG        240
CGGCAGCTTC AAGTGCCTCT TAATTGAGTC TCTTAATGTC ATCACAGCCT GGTGGTCTGA        300
TGCATTCCGA TTCCAGACAC TCAGGATGTC TTCATTGAAA CGACAACTTA GCACAATGCC        360
ACAAACATCA TCACTATAAT CAAGTTGGTC ACCCACTAGA ACCAGCACCA AATCCTCCCA        420
GAAACGCCCA GAAACAGCCT TCTTGAACCG TATAATCCAC TTGCCGCCAT TCCGATTTGC        480
AGGATCCTCC CAGAGTGGAC GGACACCCTC TTTGAAAAGA TGCAGGTCCG TGGGGCTCGG        540
TAGGGCGGAC GGG                                                           553
TypePositionOrientation
ORF 3 - 260: forward
ORF 172 - 92: reverse
ORF 551 - 399: reverse
ORF 552 - 160: reverse
ORF 553 - 392: reverse
Cluster Info:
Contig2204.2 (11 members)
view Blast Searches
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