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Clone: HX10E12

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2034
  tissue: apex
  stage/tissue: apex (3-5 mm in size)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os06g39140.1 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
Hv.264 unigene cluster
Os.12297 unigene cluster
Sbi.5152 unigene cluster
Zm.67219 unigene cluster
Sof.5975 unigene cluster
Hv.12839 unigene cluster
Hv.5459 unigene cluster
Os.90792 unigene cluster
Zm.66965 unigene cluster
Os.37672 unigene cluster
Sof.20573 unigene cluster
Sbi.837 unigene cluster
Zm.94920 unigene cluster
Sbi.18522 unigene cluster
Sof.21432 unigene cluster
Os.8864 unigene cluster
Bra.1732 unigene cluster
Bol.3778 unigene cluster
Bna.15209 unigene cluster
Bna.16698 unigene cluster
Hv.22922 unigene cluster
Can.679 unigene cluster
Vvi.9214 unigene cluster
Les.19407 unigene cluster
At.7367 unigene cluster
Nta.6843 unigene cluster
At.24240 unigene cluster
Csi.5735 unigene cluster
Bra.28765 unigene cluster
At.19006 unigene cluster
Ccl.2011 unigene cluster
Lja.541 unigene cluster
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os06g39140.2 annotated gene (MSU 6.1)
LOC_Os06g39140.1 annotated gene (MSU 6.1)
 
Sequence: HX10E12r
submitter of sequence: Dr. Nils Stein
length: 615
priming: 5-prime
GenBankAc: CA031529
IPK CR-EST: HX10E12r
Sequence features and Clone alignments (BLAST):
Click for Menu Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX10E12r
CGGCACGAGG ATACGATACC CTTTGACCAC ACGTCCCCCG ACCGCGCGTG CTCGCCGCCC         60
GATTGGCCGG CGGGTATAAA ATGGTGGAGC GCATCGCCTG AGCCGCACGC CGCCGCGCAC        120
CGTCTTTCCA CCAACCGAGC AGTAGTCAAC TGAACGGCAG CCAACAAAGC GTCCCAGCCG        180
GTCGCTGAGA GGCGGCGCCG CCGCGGGGAC GGGCGTTCCG GGAAGAGCAG GAACGATGCA        240
GTCGCTGCAG GACAAGGCGT CGGAGTGGAG CGGCGTGGCG GCGGCCGACG CATTCGCCAT        300
CGACGAGGTC AACGTCTTCG AGGCGCTCGG CGGCACCCCG CAGCCCTTCG TCGACCTCTC        360
CACCAACTTC TACACCAGGG TTTACGAGGA CGAGGAGGAG TGGTTCCGGG AGATATTCTC        420
GGGGTCCAAG AAGGAGGACG CGATCCAGAA CCAGTACGAG TTCTTGGTGC AGCGGATGGG        480
CGGCCCGCCG CTATTCTCCC AGAGGAGAGG GCATCCTGCC TTGATAGGAC GACATCGGCC        540
ATTCCCAGTT ACCCACCGAG CTGCGGAGAG ATGGCTACAC CACATGCAGC AAGCTCTGGA        600
GACCACCCAA AGCAT                                                         615
TypePositionOrientation
ORF 1 - 150: forward
ORF 2 - 76: forward
ORF 94 - 2: reverse
ORF 236 - 613: forward
ORF 293 - 3: reverse
ORF 300 - 1: reverse
ORF 542 - 312: reverse
ORF 614 - 552: reverse
ORF 615 - 385: reverse
Cluster Info:
Contig1097.1 (21 members)
view Blast Searches
 
Sequence: HX10E12w
submitter of sequence: Dr. Nils Stein
length: 437
priming: 3-prime
GenBankAc: CB876096
IPK CR-EST: HX10E12w
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX10E12w
AACAAAAAAT GCAGTTCTAT TGCTCCCATG ATTTCTGTTC AACAAAAGAC TAACAAGATA         60
AAATACTTGT TGATGTTCGC TTAGGTAAAC ACAAAATTGG TATCAGTGAA ACAGATCTAA        120
TCCAAAGACA AGCTCGGGTC GACAGGACAG CCACTGGAGA ACACCAACTT AAAACACCCG        180
TACACGCTTC CCCATGGTGC TCTTCACGTA CAGGCACCTC ACATTCTGCC AGTTCTTCTT        240
CAGGAGGGAC ACAAGGAAGT TGACGCTCAT TTGGATGTTT TGTTGGATCT GCTTCTCGTC        300
CATCGACAAG TTGCCCACAG CAACACCCAT GCAAAGAACC TTCTTTAGCT GGAACTTGAC        360
TGTAGCTTTT GTCTCGTTCA CCTTGGCTTC AAGAGATTCC TGGTGACTTA CCAGAGTGGG        420
AAACTTGCCT GCCTTGT                                                       437
TypePositionOrientation
ORF 1 - 81: forward
ORF 194 - 403: forward
ORF 435 - 172: reverse
Cluster Info:
Contig18.3 (64 members)
view Blast Searches
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