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Clone: HX09F08

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2034
  tissue: apex
  stage/tissue: apex (3-5 mm in size)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
Hv.22922 unigene cluster
Os.11487 unigene cluster
Os.8864 unigene cluster
Sof.5084 unigene cluster
Zm.138249 unigene cluster
Sbi.19782 unigene cluster
Sbi.837 unigene cluster
Zm.66965 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Zm.94920 unigene cluster
Hv.12839 unigene cluster
Sof.20573 unigene cluster
Hv.5459 unigene cluster
Bol.1747 unigene cluster
Os.37672 unigene cluster
Can.3824 unigene cluster
Bol.5797 unigene cluster
At.24240 unigene cluster
Pth.5660 unigene cluster
Ptp.5768 unigene cluster
Bol.3778 unigene cluster
Tcc.1562 unigene cluster
Les.19407 unigene cluster
Bra.1732 unigene cluster
Bna.4011 unigene cluster
At.19006 unigene cluster
Vvi.9214 unigene cluster
Tcc.7897 unigene cluster
Bna.575 unigene cluster
Ccl.2011 unigene cluster
Nta.6843 unigene cluster
Csi.5735 unigene cluster
Ptp.11065 unigene cluster
Stu.2362 unigene cluster
Les.19882 unigene cluster
Pth.17313 unigene cluster
Psi.8134 unigene cluster
Can.679 unigene cluster
Pgl.4932 unigene cluster
Nta.1653 unigene cluster
Lja.541 unigene cluster
Les.3112 unigene cluster
Ghi.5769 unigene cluster
Pta.7598 unigene cluster
Ghi.18764 unigene cluster
Bra.28765 unigene cluster
At.7367 unigene cluster
Pta.24201 unigene cluster
Gma.6491 unigene cluster
Lja.14656 unigene cluster
Ppe.20380 unigene cluster
Mtr.2748 unigene cluster
Ppe.5428 unigene cluster
Mtr.7684 unigene cluster
Les.23394 unigene cluster
Lsa.5490 unigene cluster
Ghi.4846 unigene cluster
Gma.37744 unigene cluster
Ppa.3640 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Pta.2091 unigene cluster
Ptp.634 unigene cluster
Lsa.11335 unigene cluster
Pth.16449 unigene cluster
Ppa.3917 unigene cluster
Ppa.5371 unigene cluster
Han.4573 unigene cluster
Lsa.14576 unigene cluster
Han.1824 unigene cluster
Bna.15209 unigene cluster
Can.1546 unigene cluster
Pth.16459 unigene cluster
Han.9639 unigene cluster
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
 
Sequence: HX09F08r
submitter of sequence: Dr. Nils Stein
length: 645
priming: 5-prime
GenBankAc: CA031216
IPK CR-EST: HX09F08r
Sequence features and Clone alignments (BLAST):
Click for Menu Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX09F08r
CGGCACGAGG GCTCCCTGCA TCGTCGCGGA ACAGTGCTCC CACCGCCGGC GCCATGAGTA         60
AGTTGCAGAG CGATGCCCTG AGGGAGGCTA TCACAAACAT TACCAATGAC TGCCGTGAGA        120
AGAACCGCAA GTTCGTTGAA ACCATTGAGC TGCAGATTGG TCTGAAGAAC TATGACCCGC        180
AAAAGGACAA ACGTTTCAGT GGCTCTGTTA AGCTTCCTCA CATCCCTCGC CCAAAGATGA        240
GGGTTTGCAT GCTTGGTGAT GCCCAGCATG TTGAAGAGGC AGGGAAAATG GGCCTTGACT        300
GTATGGATGT GGAATCGCTG AAGAAGATGA ACAAAAACAA GAAGCTAGTC AAGAAGCTTG        360
CAAAGAAGTA TCATGCTTTC CTTGCATCTG AGGCTATCAT TAAGCAGATT CCCCGACTTC        420
TTGGACCTGG TCTTAACAAG GCAGGAAAGT TTCCAACTTT GGTGTCTCAC CAGGAGTCCC        480
TTGAGTCCAA GGTTAATGAG ACCAAGGCCA CTGTCAAGTT CCAGTTGAAA AAGGTGCTTT        540
GCATGGGTGT TGCCGTTGGC CACCTGGGTA TGGAGGAGAA GCAGATCTTC CAGAACGTGC        600
AGATGAGCGT GAACTTCCTT GTCTCTCTGT TGAAGAAGAA TTGGC                        645
TypePositionOrientation
ORF 54 - 644: forward
ORF 145 - 2: reverse
ORF 172 - 237: forward
ORF 269 - 3: reverse
ORF 373 - 525: forward
ORF 643 - 470: reverse
ORF 644 - 348: reverse
ORF 645 - 517: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
 
Sequence: HX09F08w
submitter of sequence: Dr. Nils Stein
length: 662
priming: 3-prime
GenBankAc: CB875781
IPK CR-EST: HX09F08w
Sequence features and Clone alignments (BLAST):
Click for Menu Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX09F08w
AAAATGTCAT GAAGTTCCAG ACAATAGAAC AAACCATCTC AGTACAAGAC AATAATTGTC         60
ACTCGACGCG CACGAAAATT TGATAATTGA GAAAGCAACA AAAGTTCCCA ANGTTGTCAA        120
TCTCCTGGGA AATCCTAGAA GACTAGAGTC TAGAACAACC GGTACACCTT TCCCATTGTG        180
CTCTTCAGGT ACAGGCACCG CACGTTTTGC CAATTCTTCT TCAACAGAGA GACAAGGAAG        240
TTCACGCTCA TCTGCACGTT CTGGAAGATC TGCTTCTCCT CCATACCCAG GTGGCCAACG        300
GCAACACCCA TGCAAAGCAC CTTTTTCAAC TGGAACTTGA CAGTGGCCTT GGTCTCATTA        360
ACCTTGGACT CAAGGGACTC CTGGTGAGAC ACCAAAGTTG GAAACTTTCC TGCCTTGTTA        420
AGACCAGGTC CAAGAAGTCG GGGAATCTGC TTAATGATAG CCTCAGATGC AAGGAAAGCA        480
TGATACTTCT TTGCAAGCTT CTTGACTAGC TTCTTGTTTT TGTTCATCTT CTTCAGCGAT        540
TCCACATCCA TACAGTCAAG GCCCATTTTC CCTGCCTCTT CAACATGCTG GGCATCACCA        600
AGCATGCAAA CCCTCATCTT TGGGCGAGGG ATGTGAGGAA GCTTAACAGA GCCACTGAAA        660
CG                                                                       662
TypePositionOrientation
ORF 3 - 80: forward
ORF 310 - 384: forward
ORF 481 - 329: reverse
ORF 585 - 662: forward
ORF 662 - 153: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
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