•Home    •Contact   •FAQs   •Imprint   •Login  
 
 
 
 
 
 
 
 
 
   
 
   
 
  
 
   

Help us improve the primary database. What do you think and where should we go in the future: https://www.surveymonkey.com/s/RK3GMPC?

 search 
 for 
Help

Clone: HS08J21

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2030
  tissue: embryo + scutellum
  stage/tissue: 0-16 hours after imbibition
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Zm.9 unigene cluster
Sof.20462 unigene cluster
Sbi.17905 unigene cluster
Sbi.14146 unigene cluster
Hv.6171 unigene cluster
 
Sequence: HS08J21r
submitter of sequence: Dr. Nils Stein
length: 204
priming: 5-prime
GenBankAc: CA002817
IPK CR-EST: HS08J21r 
HS08J21r
ACCAAGCTCC GAATCCCTCC CGTCTCTACC CCCATCGAGC TCGCAAGTTC TTGCTTGGAG         60
CAGCCGAGCC GAGCTCAGAT TCCCCGCGGG GCCCCGGGAG TACTCGAGGT CTTGGTGTGA        120
GGCCAAGAAA TGGCGGAGGG GGAGGAGACC AAGGTGGAGG GAACGAGAGA GGAAGAGAGG        180
ATGGATGCAG GGGCGGGGGC GGAG                                               204
TypePositionOrientation
ORF 1 - 117: forward
ORF 130 - 204: forward
ORF 202 - 29: reverse
ORF 203 - 78: reverse
Cluster Info:
Contig2173.1 (9 members)
view Blast Searches
 
Sequence: HS08J21u
submitter of sequence: Dr. Nils Stein
length: 510
priming: 3-prime
GenBankAc: CA000428
IPK CR-EST: HS08J21u
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HS08J21u
GAGGCTGAGA CAATATCTGG GCACAATTTA TTATCAAACC AGAGCACGGG AATGCTCATT         60
TAAGTACAAG ATTCGACAGG ATCGCAAGCA CAAGCCTGAA CATTCAACAC CAGGACACAT        120
CACAAGAACA CACACTAGGA AAGGCACACA TCGGGGGTGA CACACCTCGG CTTATTTCGA        180
CGAACAAGCC CCAGAAACTA AGATCATGGC TTCATCAGCG CAGCGACCTT CTGGACGGAC        240
GGCCTCGTCA GCAGGTCAGT CCACCAGGCC TTCACATGCG GGTAAGCGTC AAACAGAGAT        300
GCATAGGGTG TGGCCGCCAA GCACAGGGTG ACAGACGCAT GGTTAAGGTC CGCAAGGCTG        360
AGGAAGTCTC CAGCCAGGTA CTTGGACTTG GTGAGCCGCG CCTCGTACAC CGCCAGCACG        420
TTCTTGATCT TAACAAGGTT GTCGTCGATG ACCTTCTGGT CAGTGGCTCC CCCAAGCATA        480
GGATGGATAA GGCACTCGAA GAGAATGGGG                                         510
TypePositionOrientation
ORF 2 - 157: forward
ORF 120 - 37: reverse
ORF 206 - 328: forward
ORF 276 - 509: forward
ORF 448 - 510: forward
ORF 508 - 275: reverse
ORF 509 - 207: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
GabiPD Webmaster