•Home    •Contact   •FAQs   •Imprint   •Login  
 
 
 
 
 
 
 
 
 
   
 
   
 
  
 
   

Help us improve the primary database. What do you think and where should we go in the future: https://www.surveymonkey.com/s/RK3GMPC?

 search 
 for 
Help

Clone: HM07L13

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2026
  tissue: male inflorescences
  stage/tissue: male inflorescences (approx. 2 mm in size), green anther stage
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
Hv.12839 unigene cluster
Hv.5459 unigene cluster
Zm.66965 unigene cluster
Os.37672 unigene cluster
Sbi.837 unigene cluster
Sof.20573 unigene cluster
Sbi.18522 unigene cluster
Zm.94920 unigene cluster
Os.8864 unigene cluster
Zm.138249 unigene cluster
Os.11487 unigene cluster
Bra.1732 unigene cluster
Bna.15209 unigene cluster
Bol.3778 unigene cluster
Hv.22922 unigene cluster
Vvi.9214 unigene cluster
Bna.16698 unigene cluster
At.24240 unigene cluster
Bra.28765 unigene cluster
Bol.5797 unigene cluster
Bol.1747 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bna.4011 unigene cluster
At.7367 unigene cluster
Tcc.1562 unigene cluster
At.19006 unigene cluster
Can.679 unigene cluster
Ghi.18764 unigene cluster
Les.19407 unigene cluster
Csi.5735 unigene cluster
Ghi.5769 unigene cluster
Ccl.2011 unigene cluster
Stu.2362 unigene cluster
Lja.541 unigene cluster
Ppa.3640 unigene cluster
Tcc.3944 unigene cluster
Can.3824 unigene cluster
Han.5727 unigene cluster
Hv.23072 unigene cluster
Zm.80765 unigene cluster
Sbi.12403 unigene cluster
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
 
Sequence: HM07L13r
submitter of sequence: Dr. Nils Stein
length: 498
priming: 5-prime
GenBankAc: BU994642
IPK CR-EST: HM07L13r
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HM07L13r
GCTTTCCTGG CATCAGAGGC CATAATCAAG CAGATCCCTC GTCTCCTTGG CCCTGGTCTT         60
AACAAGGCAG GCAAGTTTCC CACTCTGGTA AGTCACCAGG AATCTCTTGA AGCCAAGGTG        120
AACGAGACAA AAGCTACAGT CAAGTTCCAG CTAAAGAAGG TTCTTTGCAT GGGTGTTGCT        180
GTGGGCAACT TGTCGATGGA CGAGAAGCAG ATCCAACAAA ACATCCAAAT GAGCGTCAAC        240
TTCCTTGTGT CCCTCCTGAA GAAGAACTGG CAGAATGTGA GGTGCCTGTA CGTGAAGAGC        300
ACCATGGGGA AGCGTGTACG GGTGTTCTAA GTTGGTGTTC TCCAGTGGCT GTCCTGTCGA        360
CCCGAGCTTG TCTTTGGATT AGATCTGTTT CACTGATACC AATTTTGTGT TTACCTAAGC        420
GAACATCAAC AAGTATTTTA TCTTGTTAGT CTTTTGTTGA ACAGAAATCA TGGGAGCAAT        480
AGAACTGCAT TTTCTGTT                                                      498
TypePositionOrientation
ORF 1 - 327: forward
ORF 305 - 96: reverse
ORF 498 - 418: reverse
Cluster Info:
Contig18.3 (64 members)
view Blast Searches
 
Sequence: HM07L13w
submitter of sequence: Dr. Nils Stein
length: 524
priming: 3-prime
GenBankAc: CB880755
IPK CR-EST: HM07L13w
Sequence features and Clone alignments (BLAST):
Click for Menu Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HM07L13w
CCCCCCCGCG CGCGGCGGCC TCGTTCCGGG GGGTCTCACC GCCGGCGCGG CGGTCCGTGG         60
CGGCGCGGGC CGCGCTGGAG CCGTCGGTGG TGATCAGCCT CAGCACGGGG CTGTCGCTCG        120
TGATGGGCCG CTTCGTCTTC TTCAACTTCC AGCGGGAGAA CGTGGCGAAG CAGGTCCCCG        180
AGCAGAACGG CAAGACCCAC TTCGAGGCCG GCGACGAGCG CGCCAAGGAG TTCGCCGGGA        240
TCCTCAAGTC CAACGACCCC GTCGGCTTCA ACCTCGTCGA CGTCCTCGCA TGGGGCTCCA        300
TCGGCCACAT CGTCGCCTAC TACATCCTCG CCACCACCAG CAACGGATAC GACCCTCCCT        360
TCTTCGGCTG ATCCGTCCGT CTCCCCTGCC TGCCTGCGTG CCGGCCGGAG CGGCTTTGCT        420
GTTGTAATCC GCCGTGTTTT CATTAGTTCT GTATACGCTG ATGATGTACC ATCATGAGTA        480
TGTATGTATG TATGTGAGAT GGTGAAAGAT TGATCTGGAC AAGT                         524
TypePositionOrientation
ORF 1 - 90: forward
ORF 2 - 424: forward
ORF 3 - 368: forward
ORF 310 - 2: reverse
ORF 523 - 446: reverse
ORF 524 - 39: reverse
Cluster Info:
Contig40620.1 (29 members)
view Blast Searches
GabiPD Webmaster