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Clone: HF24N14

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2022
  tissue: caryopsis
  stage/tissue: developing caryopsis, 16-25 DAF (days after flowering)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
Hv.22922 unigene cluster
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Hv.25032 unigene cluster
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Tcc.7897 unigene cluster
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Vvi.9214 unigene cluster
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Ccl.2011 unigene cluster
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Pth.5660 unigene cluster
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Csi.5735 unigene cluster
Lja.541 unigene cluster
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Ptp.11065 unigene cluster
Les.23394 unigene cluster
Ghi.18764 unigene cluster
Tcc.3944 unigene cluster
Mtr.16898 unigene cluster
Gma.37744 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
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Pth.17313 unigene cluster
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LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
 
Sequence: HF24N14r
submitter of sequence: Dr. Nils Stein
length: 542
priming: 5-prime
GenBankAc: BU990377
IPK CR-EST: HF24N14r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HF24N14r
CCCCCCTCCC TGCATCGTCG CGGAACAGTG CTCCCACCGC CGGCGCCATG AGTAAGTTGC         60
AGAGCGATGC CCTGAGGGAG GCTATCACAA ACATTACCAA TGACTGCCGT GAGAAGAACC        120
GCAAGTTCGT TGAAACCATT GAGCTGCAGA TTGGTCTGAA GAACTATGAC CCGCAAAAGG        180
ACAAACGTTT CAGTGGCTCT GTTAAGCTTC CTCACATCCC TCGCCCAAAG ATGAGGGTTT        240
GCATGCTTGG TGATGCCCAG CATGTTGAAG AGGCAGGGAA AATGGGCCTT GACTGTATGG        300
ATGTGGAATC GCTGAAGAAG ATGAACAAAA ACAAGAAGCT AGTCAAGAAG CTTGCAAAGA        360
AGTATCATGC TTTCCTTGCA TCTGAGGCTA TCATTAAGCA GATTCCCCGA CTTCTTGGAC        420
CTGGTCTTAA CAAGGCAGGA AAGTTTCCAA CTTTGGTGTC TCACCAGGAG TCCCTTGAGT        480
CCAAGGTTAA TGAGACCAAG GCCACTGTCA AGTTCCAGTT GAAAAAGGTG CTTTGCATGG        540
GT                                                                       542
TypePositionOrientation
ORF 48 - 542: forward
ORF 139 - 2: reverse
ORF 166 - 231: forward
ORF 263 - 3: reverse
ORF 367 - 519: forward
ORF 541 - 464: reverse
ORF 542 - 342: reverse
Cluster Info:
Contig155.1 (81 members)
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