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Clone: HF15L14

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2022
  tissue: caryopsis
  stage/tissue: developing caryopsis, 16-25 DAF (days after flowering)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

Related with
LOC_Os01g55830.2 annotated gene (TIGR5)
LOC_Os01g55830.1 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Sbi.17905 unigene cluster
Sof.17942 unigene cluster
Sbi.17875 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HF15L14r
submitter of sequence: Dr. Nils Stein
length: 597
priming: 5-prime
GenBankAc: BU987772
IPK CR-EST: HF15L14r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HF15L14r
CGGCACGAGG GGGAGCAGCA GTGAGGCTCC ATTGCCTATT TAGCCGAGAC CCCGAGCGCG         60
GTCATTTCCC CATCCCGTCC CGTCCCATCC CATCCCATCC GAGAAGAAGA GATAGAAAAA        120
AAAAGGTTCG AGGGCGTTGC GGAGTGAGAA GAGACGAGAT GGCTCCGGTG AAGCTGTACG        180
GCGCGACCCT GTCGTGGAAC GTCACCAGGT GCGTGGCGGC GCTGGAGGAG GCCGGCGTCG        240
AGTACGAGCT CGTCCCCATC AACTTCAGCA CCGGCGAGCA CAAGAGCCCC GACCACCTCG        300
CCAGGAACCC CTTTGGGCAG GTGCCAGCTT TGCAGGATGG TGACTTGTAT GTCTTCGAAT        360
CACGTGCTAT TTGCAAGTAT GCGTGCCGCA AGAACAAGCC AGAGCTGTTG AAGGAGGGCG        420
ACATCAAGGA GTCAGCAATG GTGGATGTGT GGCTCGAGGT GGAGGCCCAT CAGTACACCG        480
CCGCTCTGAG CCCCATTCTC TTCGAGTGCC TTATCCATCC TATGCTTGGG GGAGCCACTG        540
ACCAGAAGGT CATCGACGAC AACCTTGTTA AGATCAAGAA CGTGCTGGCG GTGTACG           597
TypePositionOrientation
ORF 159 - 596: forward
ORF 337 - 408: forward
ORF 424 - 65: reverse
ORF 595 - 473: reverse
ORF 596 - 435: reverse
Cluster Info:
Contig168.1 (88 members)
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