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Clone: HF14G04

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2022
  tissue: caryopsis
  stage/tissue: developing caryopsis, 16-25 DAF (days after flowering)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
Hv.22922 unigene cluster
Os.11487 unigene cluster
Os.8864 unigene cluster
Zm.138249 unigene cluster
Sof.5084 unigene cluster
Sbi.19782 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Sbi.837 unigene cluster
Zm.66965 unigene cluster
Hv.12839 unigene cluster
Bol.1747 unigene cluster
Zm.94920 unigene cluster
Hv.5459 unigene cluster
Bol.5797 unigene cluster
Sof.20573 unigene cluster
At.24240 unigene cluster
Bra.1732 unigene cluster
Tcc.1562 unigene cluster
Vvi.9214 unigene cluster
Bol.3778 unigene cluster
Bna.4011 unigene cluster
Les.19407 unigene cluster
Os.37672 unigene cluster
Can.3824 unigene cluster
Pth.5660 unigene cluster
Ptp.5768 unigene cluster
At.19006 unigene cluster
Nta.6843 unigene cluster
Nta.1653 unigene cluster
Tcc.7897 unigene cluster
Ccl.2011 unigene cluster
Csi.5735 unigene cluster
Bna.575 unigene cluster
Stu.2362 unigene cluster
Les.19882 unigene cluster
Can.679 unigene cluster
Ghi.5769 unigene cluster
Ptp.11065 unigene cluster
Pth.17313 unigene cluster
Les.3112 unigene cluster
Lja.541 unigene cluster
Bna.15209 unigene cluster
Psi.8134 unigene cluster
Pgl.4932 unigene cluster
Ghi.18764 unigene cluster
At.7367 unigene cluster
Bra.28765 unigene cluster
Gma.6491 unigene cluster
Pta.7598 unigene cluster
Bna.2003 unigene cluster
Mtr.2748 unigene cluster
Pta.24201 unigene cluster
Lja.14656 unigene cluster
Sbi.18522 unigene cluster
Ppe.20380 unigene cluster
Lsa.5490 unigene cluster
Ppe.5428 unigene cluster
Mtr.7684 unigene cluster
Les.23394 unigene cluster
Ghi.4846 unigene cluster
Ppa.3640 unigene cluster
Gma.37744 unigene cluster
Can.1546 unigene cluster
Tcc.3944 unigene cluster
Pta.2091 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Mtr.16898 unigene cluster
Vvi.7697 unigene cluster
Ptp.634 unigene cluster
Pth.16459 unigene cluster
Han.9639 unigene cluster
Lsa.11335 unigene cluster
Pth.16449 unigene cluster
Mtr.17728 unigene cluster
Ppa.5371 unigene cluster
Lsa.14576 unigene cluster
Ppa.3917 unigene cluster
Han.4573 unigene cluster
Han.1824 unigene cluster
Nta.381 unigene cluster
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
 
Sequence: HF14G04r
submitter of sequence: Dr. Nils Stein
length: 587
priming: 5-prime
GenBankAc: BU987303
IPK CR-EST: HF14G04r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HF14G04r
AACATTACCA ATGACTGCCG TGAGAAGAAC CGCAAGTTCG TTGAAACCAT TGAGCTGCAG         60
ATTGGTCTGA AGAACTATGA CCCGCAAAAG GACAAACGTT TCAGTGGCTC TGTTAAGCTT        120
CCTCACATCC CTCGCCCAAA GATGAGGGTT TGCATGCTTG GTGATGCCCA GCATGTTGAA        180
GAGGCAGGGA AAATGGGCCT TGACTGTATG GATGTGGAAT CGCTGAAGAA GATGAACAAA        240
AACAAGAAGC TAGTCAAGAA GCTTGCAAAG AAGTATCATG CTTTCCTTGC ATCTGAGGCT        300
ATCATTAAGC AGATTCCCCG ACTTCTTGGA CCTGGTCTTA ACAAGGCAGG AAAGTTTCCA        360
ACTTTGGTGT CTCACCAGGA GTCCCTTGAG TCCAAGGTTA ATGAGACCAA GGCCACTGTC        420
AAGTTCCAGT TGAAAAAGGT GCTTTGCATG GGTGTTGCCG TTGGCCACCT GGGTATGGAG        480
GAGAAGCAGA TCTTCCAGAA CGTGCAGATG AGCGTGAACT TCCTTGTCTC TCTGTTGAAG        540
AAGAATTGGC AAAACGTGCG GTGCCTGTAC CTGAAGAGCA CAATGGG                      587
TypePositionOrientation
ORF 2 - 67: forward
ORF 77 - 142: forward
ORF 142 - 585: forward
ORF 174 - 1: reverse
ORF 278 - 430: forward
ORF 585 - 253: reverse
ORF 586 - 422: reverse
ORF 587 - 375: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
 
Sequence: RUS68E05w
submitter of sequence: Dr. Nese Sreenivasulu
length: 382
priming: 3-prime
GenBankAc: FD523851
IPK CR-EST: RUS68E05w 
RUS68E05w
AACAAACCAT CTCAGTACAA GACAATAATT GTCACTCGAC GCGCACGAAA ATTTGATAAT         60
TGAGAAAGCA ACAAAAGTTC CCAAGTTGTC AATCTCCTGG AAATCCTAGA AGACTAGAGT        120
CTAGAACAAC CGGGACACCT TTCCCATTGG GCTCTTCAGG GACAGGGACC GCACGTTTTG        180
CCAATTCTTC TTCAACAGAG AGACAAGGAA GTTCACGCTC ATCTGCACGT TCTGGAAGAT        240
CTGCTTCTCC TCCATACCCA GGGGGGCAAC GGCAACACCC ATGCAAAGCA CCTTTTTCAA        300
CTGGAACTTG ACAGTGGCCT TGGTCTCATT AACCTTGGAC TCAAGGGACT CCTGGGGAGA        360
CACCAAAGTT GGAAACTTTC CT                                                 382
TypePositionOrientation
ORF 281 - 382: forward
ORF 380 - 300: reverse
ORF 381 - 124: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
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