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Clone: HF12J06

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2022
  tissue: caryopsis
  stage/tissue: developing caryopsis, 16-25 DAF (days after flowering)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

Related with
Hv.3134 unigene cluster
Hv.932 unigene cluster
Zm.81924 unigene cluster
Sof.19777 unigene cluster
Sof.21056 unigene cluster
Sof.17942 unigene cluster
Sbi.17905 unigene cluster
 
Sequence: HF12J06r
submitter of sequence: Dr. Nils Stein
length: 196
priming: 5-prime
GenBankAc: BU986670
IPK CR-EST: HF12J06r 
HF12J06r
CGGCACGAGG CCCATCCGAT AAGAAGAGTA TAGAAAAAAA AGGTTCGAGG GCGTTGCGGA         60
GTGAGAAGAG ACGAGATGGC TCCGGTGAAG CTGTACGGCG CGACCCTGTC GTGGAACGTC        120
ACCAGCTGCG TGGCGGCGCT GGAGGAGGCC GGCGTCGAGT ACGAGCTCGT CCCCATCAAC        180
TTCAGCACCG GCGAGC                                                        196
TypePositionOrientation
ORF 76 - 195: forward
ORF 176 - 3: reverse
Cluster Info:
Contig168.1 (88 members)
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