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Clone: HF03O10

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2022
  tissue: caryopsis
  stage/tissue: developing caryopsis, 16-25 DAF (days after flowering)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

Related with
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
Hv.12839 unigene cluster
Hv.25032 unigene cluster
Os.37672 unigene cluster
Zm.66965 unigene cluster
Sof.20573 unigene cluster
Sbi.837 unigene cluster
Zm.94920 unigene cluster
Hv.5459 unigene cluster
Sbi.18712 unigene cluster
Os.11487 unigene cluster
Hv.22922 unigene cluster
Os.8864 unigene cluster
Sof.5084 unigene cluster
Zm.138249 unigene cluster
Sbi.18522 unigene cluster
Sbi.19782 unigene cluster
At.19006 unigene cluster
Bol.1747 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bra.1732 unigene cluster
Bol.3778 unigene cluster
Bna.575 unigene cluster
Bol.5797 unigene cluster
Ccl.2011 unigene cluster
Csi.5735 unigene cluster
At.7367 unigene cluster
Zm.127093 unigene cluster
Vvi.9214 unigene cluster
At.24240 unigene cluster
Bna.4011 unigene cluster
Can.1546 unigene cluster
Bra.28765 unigene cluster
Bna.15209 unigene cluster
Tcc.1562 unigene cluster
Tcc.7897 unigene cluster
Ppe.20380 unigene cluster
Psi.6078 unigene cluster
Ppe.5428 unigene cluster
Ppa.3640 unigene cluster
Pgl.14502 unigene cluster
Les.3112 unigene cluster
Han.5727 unigene cluster
Bna.2003 unigene cluster
Les.19882 unigene cluster
Lja.541 unigene cluster
Mtr.7684 unigene cluster
Les.23394 unigene cluster
Can.3824 unigene cluster
Can.679 unigene cluster
Stu.2362 unigene cluster
Lja.14656 unigene cluster
Lsa.5490 unigene cluster
Nta.1653 unigene cluster
Ghi.18764 unigene cluster
Mtr.2748 unigene cluster
Nta.6843 unigene cluster
Psi.8134 unigene cluster
Pgl.4932 unigene cluster
Han.9639 unigene cluster
Pta.7598 unigene cluster
Les.19407 unigene cluster
Gma.6491 unigene cluster
Pta.24201 unigene cluster
Ghi.5769 unigene cluster
Ghi.4846 unigene cluster
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
 
Sequence: HF03O10r
submitter of sequence: Dr. Nils Stein
length: 596
priming: 5-prime
GenBankAc: BU984427
IPK CR-EST: HF03O10r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HF03O10r
CAACTGATCG GCGGCAGCCA TGAGTAAGCT CCAAAGTGAT GCTGTCAAGG ATGCCATCAC         60
CCAGATTGTT GGGGAGGCCA GAGAAAAGAA ACGCAAGTTC ACTGAGACTG TGGAGCTTCA        120
GATTGGTCTC AAGAACTACG ACCCACAAAA GGATAAACGT TTCAGTGGCT CTGTGAAGCT        180
GCCCCATATC CCCCGCCCAA AGATGAGGGT GTGCATGCTT GGTGATGCCC AGCACGTTGA        240
CCAGGCTGAG AAAATGGGAC TTGACTACAT GGATGTTGAA AGTCTTAAGA AGATGAACAA        300
GAACAAGAAG CTTGTTAAGA AGCTAGCCAA GAAGTACCAT GCTTTCCTGG CATCAGAGGC        360
CATAATCAAG CAGATCCCTC GTCTCCTTGG CCCTGGTCTT AACAAGGCAG GCAAGTTTCC        420
CACTCTGGTA AGTCACCAGG AATCTCTTGA AGCCAAGGTG AACGAGACAA AAGCTACAGT        480
CAAGTTCCAG CTAAAGAAGG TTCTTTGCAT GGGTGTTGCT GTGGGCAACT TGTCGATGGA        540
CGAGAAGCAG ATCCAACAAA ACATCCAAAT GAGCGTCAAC TTCCTTGTGT CCCTCC            596
TypePositionOrientation
ORF 20 - 595: forward
ORF 39 - 173: forward
ORF 187 - 2: reverse
ORF 216 - 139: reverse
ORF 225 - 293: forward
ORF 363 - 268: reverse
ORF 594 - 436: reverse
ORF 595 - 494: reverse
Cluster Info:
Contig18.3 (64 members)
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