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Clone: HD12F11

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Golden Promise
Library:
  lib name: IPKc 2020
  tissue: callus
  stage/tissue: callus (5-10 mm in diameter)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

Related with
LOC_Os01g55830.2 annotated gene (TIGR5)
LOC_Os01g55830.1 annotated gene (TIGR5)
Hv.3134 unigene cluster
Hv.932 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Sbi.17905 unigene cluster
Sof.17942 unigene cluster
Sof.19777 unigene cluster
Sbi.17875 unigene cluster
Sof.20462 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HD12F11r
submitter of sequence: Dr. Nils Stein
length: 376
priming: 5-prime
GenBankAc: BU993023
IPK CR-EST: HD12F11r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
HD12F11r
CGGCACGAGG AAGAGATAGA AAAAAAGGTT CGAGGGCGTT GCGGAGTGAG AAGAGACGAG         60
ATGGCTCCGG TGAAGCTGTA CGGCGCGACC CTGTCGTGGA ACGTCACCAG GTGCGTGGCG        120
GCGCTTGAGG AGGCCGGCGT CGAGTACGAG CTCGTCCCCA TCAACTTCGG CACCGGCGAG        180
CACAAGAGCC CCGACCACCT CGCCAGGAAC CCCTTTGGGC AGGTGCCAGC TTTGCAGGAT        240
GGTGACTTGT ACGTCTTCGA ATCACGTGCT ATTTGCAAGT ATGCGTGCCG CAAGAACAAG        300
CCAGAGCTGT TGAAGGAGGG TGACATCAAG GAGTCAGCAA TGGTGGATGT GTGGCTCGAG        360
GTGGAGGCCC ATCAGT                                                        376
	      
TypePositionOrientation
ORF 3 - 125: forward
ORF 61 - 375: forward
ORF 239 - 310: forward
ORF 326 - 3: reverse
Cluster Info:
Contig168.1 (88 members)
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