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Clone: HD05G15

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Golden Promise
Library:
  lib name: IPKc 2020
  tissue: callus
  stage/tissue: callus (5-10 mm in diameter)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
LOC_Os01g55830.2 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Sbi.17905 unigene cluster
Zm.9 unigene cluster
Sbi.17875 unigene cluster
Sof.19777 unigene cluster
Sof.20462 unigene cluster
Sbi.18265 unigene cluster
Sbi.14146 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HD05G15r
submitter of sequence: Dr. Nils Stein
length: 631
priming: 5-prime
GenBankAc: BU990909
IPK CR-EST: HD05G15r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HD05G15r
CGGCACGAGG AAGAGATAGA AAAAAAAGGT TCGAGGGCGT TGCGGAGTGA GAAGAGACGA         60
GATGGCTCCG GTGAAGCTGT ACGGCGCGAC CCTGTCGTGG AACGTCACCA GGTGCGTGGC        120
GGCGCTTGAG GAGGCCGGCG TCGAGTACGA GCTCGTCCCC ATCAACTTCG GCACCGGCGA        180
GCACAAGAGC CCCGACCACC TCGCCAGGAA CCCCTTTGGG CAGGTGCCAG CTTTGCAGGA        240
TGGTGACTTG TACGTCTTCG AATCACGTGC TATTTGCAAG TATGCGTGCC GCAAGAACAA        300
GCCAGAGCTG TTGAAGGAGG GTGACATCAA GGAGTCAGCA ATGGTGGATG TGTGGCTCGA        360
GGTGGAGGCC CATCAGTACA CCGCCGCTCT GAGCCCCATT CTCTTCGAGT GCCTTATCCA        420
TCCTATGCTT GGGGGAGCCA CTGACCAGAA GGTCATCGAC GACAACCTTG TTAAGATCAA        480
GAATGTGCTG GCGGTGTACG AGGCGCGGCT CACCAAGTCC AAGTACCTGG CTGGAGACTT        540
CCTCAGCCTT GCGGACCTTA ACCATGCGTC TGTCACCCTG TGCTTGGCGG CCACACCCTA        600
TGCATCTCTG TTCGACGCTT ACCCGCATGT G                                       631
TypePositionOrientation
ORF 1 - 126: forward
ORF 62 - 631: forward
ORF 240 - 311: forward
ORF 327 - 1: reverse
ORF 456 - 376: reverse
ORF 483 - 629: forward
ORF 631 - 338: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
 
Sequence: HD05G15w
submitter of sequence: Dr. Nils Stein
length: 567
priming: 3-prime
GenBankAc: CB864212
IPK CR-EST: HD05G15w
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HD05G15w
GAGACAATAT CTGGGCACAA TTTATTATCA AACCAGAGCA CGGGAATGCT CATTTAAGTA         60
CAAGATTCGA CAGGATCGCA AGCACAAGCC TGAACATTCA ACACCAGGAC ACATCACAAG        120
AACACACACT AGGAAAGGCA CACATCGGGG GTGACACACC TCGGCTTATT TCGACGAACA        180
AGCCCCAGAA ACTAAGATCA TGGCTTCATC AGCGCAGCGA CCTTCTGGAC GGACGGCCTC        240
GTCAGCAGGT CAGTCCACCA GGCCTTCACA TGCGGGTAAG CGTCGAACAG AGATGCATAG        300
GGTGTGGCCG CCAAGCACAG GGTGACAGAC GCATGGTTAA GGTCCGCAAG GCTGAGGAAG        360
TCTCCAGCCA GGTACTTGGA CTTGGTGAGC CGCGCCTCGT ACACCGCCAG CACATTCTTG        420
ATCTTAACAA GGTTGTCGTC GATGACCTTC TGGTCAGTGG CTCCCCCAAG CATAGGATGG        480
ATAAGGCACT CGAAGAGAAT GGGGCTCAGA GCGGCGGTGT ACTGATGGGC CTCCACCTCG        540
AGCCACACAT CCACCATTGC TGACTCC                                            567
TypePositionOrientation
ORF 2 - 151: forward
ORF 3 - 560: forward
ORF 114 - 31: reverse
ORF 200 - 322: forward
ORF 415 - 269: reverse
ORF 442 - 522: forward
ORF 566 - 201: reverse
ORF 567 - 457: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
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