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Clone: HB12A08

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2019
  tissue: developing caryopsis
  stage/tissue: 8-15 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 8-15 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
Hv.13426 unigene cluster
Hv.23678 unigene cluster
 
Sequence: HB12A08r
submitter of sequence: Dr. Nils Stein
length: 515
priming: 5-prime
GenBankAc: BU969609
IPK CR-EST: HB12A08r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HB12A08r
GGGCACGAGG ATCCACTCCC GCACATCCTC AACAATGGCA GATGCAGCGC TCAGGGACCG         60
GCTGAACACC CTTGTCACAT CGATGTTCGC CACGGGCATG CTGGACGAAA ATTTCCACCA        120
ACTCAAGTCG ATGGAGGAGG ATGGCACCGC AGCCCCGGGC TACGTCGCCG ACATCATCAA        180
CCTCTTCATC AGCAACACCA AAAGGATCCT CAACGACATC GCCGCCCTGC TGAACCAGCC        240
CGTGGTTGAC TTCGACATGG TGGACGTCCT AGTGCGTCAG CTCAAGGGGT GCAGCTACAG        300
TGTTGGTGCT AAGAAAGTGA ATCACTCCTG CATGCAATTC CGTCGGTTCT ATGAGGCAAG        360
AAGCAAAGAA AGGTGCCTCA TGGCGTTGAA CCTTGCTTGG AATGAGTTCT CTGATGTGCG        420
CAGCAAGTTC GAGACTATGA TGCAGCTGGA GGAGCAGATC GCCGCTTATG GTCCAAAGTG        480
GTAGCAATGG AAGGAAAAAT AGCATGGCTA CATAT                                   515
TypePositionOrientation
ORF 1 - 246: forward
ORF 2 - 481: forward
ORF 99 - 1: reverse
ORF 141 - 230: forward
ORF 258 - 163: reverse
ORF 514 - 272: reverse
ORF 515 - 438: reverse
Cluster Info:
Contig12999.1 (3 members)
Representative for Uniset
view Blast Searches
 
Sequence: RUS36B08w
submitter of sequence: Dr. Nese Sreenivasulu
length: 627
priming: 3-prime
GenBankAc: FD519132
IPK CR-EST: RUS36B08w 
RUS36B08w
AAGTTTTGTT TGAAGACTTG CGAGGATGGA ATTCCTACGC AAGATTCCTC ATGTTCTTTC         60
ATAATCGCGC ACAAAAGCCA GACACATATT GAACATTGTC TACATCTGCA GAGATACATA        120
GACCCCATCA TGTAAGGAAT CATATGTAGC CATGCTATTT TTCCTTCCAT TGCTACCACT        180
TTGGACCATA AGCGGCGATC TGCTCCTCCA GCTGCATCAT AGTCTCGAAC TTGCTGCGCA        240
CATCAGAGAA CTCATTCCAA GCAAGGTTCA ACGCCATGAG GCACCTTTCT TTGCTTCTTG        300
CCTCATAGAA CCGACGGAAT TGCATGCAGG AGTGATTCAC TTTCTTAGCA CCAACACTGT        360
AGCTGCACCC CTTGAGCTGA CGCACTAGGA CGTCCACCAT GTCGAAGTCA ACCACGGGCT        420
GGTTCAGCAG GGCGGCGATG TCGTTGAGGA TCCTTTTGGT GTTGCTGATG AAGAGGTTGA        480
TGATGTCGGC GACGTAGCCC GGGGCTGCGG TGCCATCCTC CTCCATCGAC TTGAGTTGGT        540
GGAAATTTTC GTCCAGCATG CCCGTGGCGA ACATCGATGT GACAAGGGTG TTCAGCCGGT        600
CCCTGAGCGC TGCATCTGCC ATTGTTG                                            627
TypePositionOrientation
ORF 1 - 132: forward
ORF 3 - 89: forward
ORF 152 - 385: forward
ORF 170 - 3: reverse
ORF 189 - 103: reverse
ORF 399 - 494: forward
ORF 516 - 427: reverse
ORF 558 - 626: forward
ORF 625 - 176: reverse
ORF 626 - 411: reverse
Cluster Info:
Contig12999.1 (3 members)
view Blast Searches
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