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Clone: HA27J15

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
Hv.12839 unigene cluster
Hv.5459 unigene cluster
Os.90792 unigene cluster
Os.8864 unigene cluster
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
 
Sequence: HA27J15r
submitter of sequence: Dr. Nils Stein
length: 360
priming: 5-prime
GenBankAc: BU982783
IPK CR-EST: HA27J15r 
HA27J15r
CGGCACGAGG AGCTAAAGAA GGTTCTTTGC ATGGGTGTTG CTGTGGGCAA CTTGTCGATG         60
GACGAGAAGC AGATCCAACA AAACATCCAA ATGAGCGTCA ACTTCCTTGT GTCCCTCCTG        120
AAGAAGAACT GGCAGAATGT GAGGTGCCTG TACGTGAAGA GCACCATGGG GAAGCGTGTA        180
CGGGTGTTCT AAGTTGGTGT TCTCCAGTGG CTGTCCTGTC GACCCGAGCT TGTCTTTGGA        240
TTAGATCTGT TTCACTGATA CCAATTTTGT GTTTACCTAA GCGAACATCA ACAAGTATTT        300
TATCTTGTTA GTCTTTTGTT GAACAGAAAT CATGGGAGCA ATAGAACTGC ATTTTCTGTT        360
TypePositionOrientation
ORF 1 - 189: forward
ORF 3 - 308: forward
ORF 167 - 3: reverse
ORF 360 - 280: reverse
Cluster Info:
Contig18.3 (64 members)
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