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Clone: HA14I18

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
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Hv.25032 unigene cluster
Sbi.18712 unigene cluster
Tcc.1562 unigene cluster
Tcc.7897 unigene cluster
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Vvi.9214 unigene cluster
Pgl.4932 unigene cluster
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Ccl.2011 unigene cluster
Stu.2362 unigene cluster
Bra.1732 unigene cluster
Pth.5660 unigene cluster
Nta.6843 unigene cluster
Csi.5735 unigene cluster
Lja.541 unigene cluster
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Ptp.11065 unigene cluster
Les.23394 unigene cluster
Ghi.18764 unigene cluster
Tcc.3944 unigene cluster
Mtr.16898 unigene cluster
Gma.37744 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Lsa.5490 unigene cluster
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Pth.17313 unigene cluster
Pta.2091 unigene cluster
Mtr.17728 unigene cluster
Lsa.11335 unigene cluster
Lsa.14576 unigene cluster
Han.4573 unigene cluster
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
 
Sequence: HA14I18r
submitter of sequence: Dr. Nils Stein
length: 554
priming: 5-prime
GenBankAc: BU978883
IPK CR-EST: HA14I18r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA14I18r
CGGCACGAGG CTCCCCCCTC CCTGCATCGT CGCGGAACAG TGCTCCCACC GCCGGCGCCA         60
TGAGTAAGTT GCAGAGCGAT GCCCTGAGGG AGGCTATCAC AAACATTACC AATGACTGCC        120
GTGAGAAGAA CCGCAAGTTC GTTGAAACCA TTGAGCTGCA GATTGGTCTG AAGAACTATG        180
ACCCGCAAAA GGACAAACGT TTCAGTGGCT CTGTTAAGCT TCCTCACATC CCTCGCCCAA        240
AGATGAGGGT TTGCATGCTT GGTGATGCCC AGCATGTTGA AGAGGCAGGG AAAATGGGCC        300
TTGACTGTAT GGATGTGGAA TCGCTGAAGA AGATGAACAA AAACAAGAAG CTAGTCAAGA        360
AGCTTGCAAA GAAGTATCAT GCTTTCCTTG CATCTGAGGC TATCATTAAG CAGATTCCCC        420
GACTTCTTGG ACCTGGTCTT AACAAGGCAG GAAAGTTTCC AACTTTGGTG TCTCACCAGG        480
AGTCCCTTGA GTCCAAGGTT AATGAGACCA AGGCCACTGT CAAGTTCCAG TTGAAAAAGG        540
TGCTTTGCAT GGGT                                                          554
TypePositionOrientation
ORF 2 - 64: forward
ORF 60 - 554: forward
ORF 151 - 2: reverse
ORF 178 - 243: forward
ORF 275 - 3: reverse
ORF 379 - 531: forward
ORF 553 - 476: reverse
ORF 554 - 354: reverse
Cluster Info:
Contig155.1 (81 members)
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