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Clone: HA03H17

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
Hv.22922 unigene cluster
Os.11487 unigene cluster
Sof.5084 unigene cluster
Sbi.19782 unigene cluster
Zm.138249 unigene cluster
Os.8864 unigene cluster
Sbi.837 unigene cluster
Sof.20573 unigene cluster
Zm.94920 unigene cluster
Zm.66965 unigene cluster
Hv.12839 unigene cluster
Os.37672 unigene cluster
Hv.5459 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bna.575 unigene cluster
Can.3824 unigene cluster
Bol.1747 unigene cluster
Hv.25032 unigene cluster
Sbi.18712 unigene cluster
Tcc.1562 unigene cluster
Tcc.7897 unigene cluster
Psi.8134 unigene cluster
Bol.3778 unigene cluster
Bol.5797 unigene cluster
Vvi.9214 unigene cluster
Pgl.4932 unigene cluster
At.24240 unigene cluster
Ptp.5768 unigene cluster
Ccl.2011 unigene cluster
Stu.2362 unigene cluster
Bra.1732 unigene cluster
Pth.5660 unigene cluster
Nta.6843 unigene cluster
Csi.5735 unigene cluster
Lja.541 unigene cluster
At.19006 unigene cluster
Les.19407 unigene cluster
Les.19882 unigene cluster
Bna.2003 unigene cluster
Pta.7598 unigene cluster
Bna.4011 unigene cluster
Pta.24201 unigene cluster
Lja.14656 unigene cluster
Ppe.20380 unigene cluster
Les.3112 unigene cluster
At.7367 unigene cluster
Gma.6491 unigene cluster
Mtr.7684 unigene cluster
Mtr.2748 unigene cluster
Ghi.5769 unigene cluster
Nta.1653 unigene cluster
Ptp.11065 unigene cluster
Les.23394 unigene cluster
Ghi.18764 unigene cluster
Tcc.3944 unigene cluster
Mtr.16898 unigene cluster
Gma.37744 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Lsa.5490 unigene cluster
Ghi.4846 unigene cluster
Vvi.7697 unigene cluster
Pth.17313 unigene cluster
Pta.2091 unigene cluster
Mtr.17728 unigene cluster
Lsa.11335 unigene cluster
Lsa.14576 unigene cluster
Han.4573 unigene cluster
Can.679 unigene cluster
Bna.15209 unigene cluster
Bra.28765 unigene cluster
Sbi.18522 unigene cluster
Bna.16698 unigene cluster
Can.1546 unigene cluster
Ptp.634 unigene cluster
Ppa.3640 unigene cluster
Pth.16459 unigene cluster
Pth.16449 unigene cluster
Vvi.24840 unigene cluster
Han.5727 unigene cluster
Ppa.3917 unigene cluster
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
 
Sequence: HA03H17r
submitter of sequence: Dr. Nils Stein
length: 550
priming: 5-prime
GenBankAc: BU976299
IPK CR-EST: HA03H17r
Sequence features and Clone alignments (BLAST):
Click for Menu Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA03H17r
CGGCACGAGG CCCCTCCCTG CATCGTCGCG GAACAGTGCT CCCACCGCCG GCGCCATGAG         60
TAAGTTGCAG AGCGATGCCC TGAGGGAGGC TATCACAAAC ATTACCAATG ACTGCCGTGA        120
GAAGAACCGC AAGTTCGTTG AAACCATTGA GCTGCAGATT GGTCTGAAGA ACTATGACCC        180
GCAAAAGGAC AAACGTTTCA GTGGCTCTGT TAAGCTTCCT CACATCCCTC GCCCAAAGAT        240
GAGGGTTTGC ATGCTTGGTG ATGCCCAGCA TGTTGAAGAG GCAGGGAAAA TGGGCCTTGA        300
CTGTATGGAT GTGGAATCGC TGAAGAAGAT GAACAAAAAC AAGAAGCTAG TCAAGAAGCT        360
TGCAAAGAAG TATCATGCTT TCCTTGCATC TGAGGCTATC ATTAAGCAGA TTCCCCGACT        420
TCTTGGACCT GGTCTTAACA AGGCAGGAAA GTTTCCAACT TTGGTGTCTC ACCAGGAGTC        480
CCTTGAGTCC AAGGTTAATG AGACCAAGGC CACTGTCAAG TTCCAGTTGA AAAAGGTGCT        540
TTGCATGGGT                                                               550
TypePositionOrientation
ORF 56 - 550: forward
ORF 147 - 1: reverse
ORF 174 - 239: forward
ORF 271 - 2: reverse
ORF 375 - 527: forward
ORF 549 - 472: reverse
ORF 550 - 350: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
 
Sequence: HA03H17u
submitter of sequence: Dr. Nils Stein
length: 497
priming: 3-prime
GenBankAc: BU976300
IPK CR-EST: HA03H17u
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA03H17u
GTCATGAAGT TCCAGACAAT AGAACAAACC ATCTCAGTAC AAGACAATAA TTGTCACTCG         60
ACGCGCACGA AAATTTGATA ATTGAGAAAG CAACAAAAGT TCCCAAGTTG TCAATCTCCT        120
GGAAATCCTA GAAGACTAGA GTCTAGAACA ACCGGTACAC CTTTCCCATT GTGCTCTTCA        180
GGTACAGGCA CCGCACGTTT TGCCAATTCT TCTTCAACAG AGAGACAAGG AAGTTCACGC        240
TCATCTGCAC GTTCTGGAAG ATCTGCTTCT CCTCCATACC CAGGTGGCCA ACGGCAACAC        300
CCATGCAAAG CACCTTTTTC AACTGGAACT TGACAGTGGC CTTGGTCTCA TTAACCTTGG        360
ACTCAAGGGA CTCCTGGTGA GACACCAAAG TTGGAAACTT TCCTGCCTTG TTAAGACCAG        420
GTCCAAGAAG TCGGGGAATC TGCTTAATGA TAGCCTCAGA TGCAAGGAAA GCATGATACT        480
TCTTTGCAAG CTTCTTG                                                       497
TypePositionOrientation
ORF 1 - 75: forward
ORF 303 - 377: forward
ORF 495 - 322: reverse
ORF 496 - 146: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
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