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Clone: HA03A19

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
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Lsa.11335 unigene cluster
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LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
 
Sequence: HA03A19r
submitter of sequence: Dr. Nils Stein
length: 474
priming: 5-prime
GenBankAc: BU976046
IPK CR-EST: HA03A19r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA03A19r
CGGCACGAGG CTCTCTCGAC CGACGCCGCC GCCGCGTCGA CAAAGTCCGT AAGGGCGTCA         60
ACTGATCGGC GGCAGCCATG AGTAAGCTCC AAAGTGATGC TGTCAAGGAT GCCATCACCC        120
AGATTGTTGG GGAGGCCAGA GAAAAGAAAC GCAAGTTCAC TGAGACTGTG GAGCTTCAGA        180
TTGGTCTCAA GAACTACGAC CCACAAAAGG ATAAACGTTT CAGTGGCTCT GTGAAGCTGC        240
CCCATATCCC CCGCCCAAAG ATGAGGGTGT GCATGCTTGG TGATGCCCAG CACGTTGACC        300
AGGCTGAGAA AATGGGACTT GACTACATGG ATGTTGAAAG TCTTAAGAAG ATGAACAAGA        360
ACAAGAAGCT TGTTAAGAAG CTAGCCAAGA AGTACCATGC TTTCCTGGCA TCAGAGGCCA        420
TAATCAAGCA GATCCCTCGT CTCCTTGGCC CTGGTCTTAA CAAGGCAGGC AAGT              474
TypePositionOrientation
ORF 1 - 78: forward
ORF 78 - 473: forward
ORF 97 - 231: forward
ORF 115 - 2: reverse
ORF 245 - 3: reverse
ORF 274 - 197: reverse
ORF 283 - 351: forward
ORF 472 - 326: reverse
Cluster Info:
Contig18.3 (64 members)
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