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Clone: HX04A08

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2034
  tissue: apex
  stage/tissue: apex (3-5 mm in size)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
Sbi.18522 unigene cluster
Tcc.3944 unigene cluster
Pth.17313 unigene cluster
Hv.12839 unigene cluster
Hv.25032 unigene cluster
Zm.66965 unigene cluster
Os.37672 unigene cluster
Sof.20573 unigene cluster
Sbi.837 unigene cluster
Zm.94920 unigene cluster
Hv.5459 unigene cluster
Sbi.18712 unigene cluster
Os.11487 unigene cluster
Hv.22922 unigene cluster
Sof.5084 unigene cluster
Os.8864 unigene cluster
Zm.138249 unigene cluster
Sbi.19782 unigene cluster
At.19006 unigene cluster
Bol.1747 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bra.1732 unigene cluster
Bol.3778 unigene cluster
Bna.575 unigene cluster
Bol.5797 unigene cluster
Ccl.2011 unigene cluster
Zm.127093 unigene cluster
Vvi.9214 unigene cluster
At.7367 unigene cluster
Csi.5735 unigene cluster
At.24240 unigene cluster
Bna.4011 unigene cluster
Can.1546 unigene cluster
Bra.28765 unigene cluster
Bna.15209 unigene cluster
Tcc.1562 unigene cluster
Tcc.7897 unigene cluster
Ppe.20380 unigene cluster
Psi.6078 unigene cluster
Ppa.3640 unigene cluster
Pgl.14502 unigene cluster
Les.3112 unigene cluster
Han.5727 unigene cluster
Ppe.5428 unigene cluster
Pta.2091 unigene cluster
Bna.2003 unigene cluster
Nta.23197 unigene cluster
Les.19882 unigene cluster
Lja.541 unigene cluster
Mtr.7684 unigene cluster
Les.23394 unigene cluster
Can.3824 unigene cluster
Stu.2362 unigene cluster
Lja.14656 unigene cluster
Lsa.5490 unigene cluster
Nta.1653 unigene cluster
Mtr.2748 unigene cluster
Can.679 unigene cluster
Nta.6843 unigene cluster
Psi.8134 unigene cluster
Ghi.18764 unigene cluster
Pgl.4932 unigene cluster
Han.9639 unigene cluster
Pta.7598 unigene cluster
Gma.6491 unigene cluster
Pta.24201 unigene cluster
Lsa.11335 unigene cluster
Les.19407 unigene cluster
Ghi.5769 unigene cluster
Ghi.4846 unigene cluster
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
 
Sequence: HX04A08r
submitter of sequence: Dr. Nils Stein
length: 566
priming: 5-prime
GenBankAc: BQ470782
IPK CR-EST: HX04A08r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX04A08r
CAAAGTCCGT AAGGGCGTCA ACTGATCGGC GGCAGCCATG AGTAAGCTCC AAAGTGATGC         60
TGTCAAGGAT GCCATCACCC AGATTGTTGG GGAGGCCAGA GAAAAGAAAC GCAAGTTCAC        120
TGAGACTGTG GAGCTTCAGA TTGGTCTCAA GAACTACGAC CCACAAAAGG ATAAACGTTT        180
CAGTGGCTCT GTGAAGCTGC CCCATATCCC CCGCCCAAAG ATGAGGGTGT GCATGCTTGG        240
TGATGCCCAG CACGTTGACC AGGCTGAGAA AATGGGACTT GACTACATGG ATGTTGAAAG        300
TCTTAAGAAG ATGAACAAGA ACAAGAAGCT TGTTAAGAAG CTAGCCAAGA AGTACCATGC        360
TTTCCTGGCA TCAGAGGCCA TAATCAAGCA GATCCCTCGT CTCCTTGGCC CTGGTCTTAA        420
CAAGGCAGGC AAGTTTCCCA CTCTGGTAAG TCACCAGGAA TCTCTTGAAG CCAAGGTGAA        480
CGAGACAAAA GCTACAGTCA AGTTCCAGCT AAAGAAGGTT CTTTGCATGG GTGTTGCTGT        540
GGGCAACTTG TCGATGGACG AGAAGC                                             566
TypePositionOrientation
ORF 38 - 565: forward
ORF 57 - 191: forward
ORF 75 - 1: reverse
ORF 205 - 2: reverse
ORF 234 - 157: reverse
ORF 243 - 311: forward
ORF 381 - 286: reverse
ORF 564 - 454: reverse
ORF 566 - 501: reverse
Cluster Info:
Contig18.3 (64 members)
view Blast Searches
 
Sequence: HX04A08u
submitter of sequence: Dr. Nils Stein
length: 582
priming: 3-prime
GenBankAc: BQ665530
IPK CR-EST: HX04A08u 
HX04A08u
AAAATCAAGA TCGCATAGAT CTTTGATATA AAAGGCACAA CGGAGAATCT TAACAGAAAA         60
TGCAGTTCTA TTGCTCCCAT GATTTCTGTT CAACAAAAGA CTAACAAGAT AAAATACTTG        120
TTGATGTTCG CTTAGGTAAA CACAAAATTG GTATCAGTGA AACAGATCTA ATCCAAAGAC        180
AAGCTCGGGT CGACAGGACA GCCACTGGAG AACACCAACT TAGAACACCC GTACACGCTT        240
CCCCATGGTG CTCTTCACGT ACAGGCACCT CACATTCTGC CAGTTCTTCT TCAGGAGGGA        300
CACAAGGAAG TTGACGCTCA TTTGGATGTT TTGTTGGATC TGCTTCTCGT CCATCGACAA        360
GTTGCCCACA GCAACACCCA TGCAAAGAAC CTTCTTTAGC TGGAACTTGA CTGTAGCTTT        420
TGTCTCGTTC ACCTTGGCTT CAAGAGATTC CTGGTGACTT ACCAGAGTGG GAAACTTGCC        480
TGCCTTGTTA AGACCAGGGC CAAGGAGACG AGGGATCTGC TTGATTATGG CCTCTGATGC        540
CAGGAAAGCA TGGTACTTCT TGGCTAGCTT CTTAACAAGC TT                           582
TypePositionOrientation
ORF 80 - 3: reverse
ORF 245 - 454: forward
ORF 582 - 223: reverse
Cluster Info:
Contig18.3 (64 members)
view Blast Searches
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