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Clone: HX03N10

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2034
  tissue: apex
  stage/tissue: apex (3-5 mm in size)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
Sbi.19782 unigene cluster
Os.37672 unigene cluster
Bna.575 unigene cluster
Hv.25032 unigene cluster
Sbi.18712 unigene cluster
Bna.2003 unigene cluster
Lja.14656 unigene cluster
Ppe.20380 unigene cluster
Mtr.7684 unigene cluster
Les.23394 unigene cluster
Tcc.3944 unigene cluster
Mtr.16898 unigene cluster
Gma.37744 unigene cluster
Vvi.7697 unigene cluster
Mtr.17728 unigene cluster
Lsa.14576 unigene cluster
Han.4573 unigene cluster
Hv.22922 unigene cluster
Os.8864 unigene cluster
Zm.138249 unigene cluster
Os.11487 unigene cluster
Sof.5084 unigene cluster
Can.679 unigene cluster
Les.19407 unigene cluster
Bra.1732 unigene cluster
Bna.15209 unigene cluster
At.24240 unigene cluster
Hv.5459 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bna.4011 unigene cluster
Bol.3778 unigene cluster
Pth.5660 unigene cluster
Ptp.5768 unigene cluster
Zm.66965 unigene cluster
Bol.5797 unigene cluster
Bol.1747 unigene cluster
Vvi.9214 unigene cluster
Ptp.11065 unigene cluster
Sbi.837 unigene cluster
Nta.1653 unigene cluster
Hv.12839 unigene cluster
At.19006 unigene cluster
Can.3824 unigene cluster
Nta.6843 unigene cluster
Bra.28765 unigene cluster
Pth.17313 unigene cluster
Les.3112 unigene cluster
Tcc.1562 unigene cluster
Sbi.18522 unigene cluster
Stu.2362 unigene cluster
Les.19882 unigene cluster
Zm.94920 unigene cluster
Sof.20573 unigene cluster
Ghi.18764 unigene cluster
Can.1546 unigene cluster
At.7367 unigene cluster
Ppa.3640 unigene cluster
Csi.5735 unigene cluster
Ghi.5769 unigene cluster
Ccl.2011 unigene cluster
Lja.541 unigene cluster
Tcc.7897 unigene cluster
Lsa.5490 unigene cluster
Pth.16459 unigene cluster
Psi.8134 unigene cluster
Pgl.4932 unigene cluster
Ppa.5371 unigene cluster
Gma.6491 unigene cluster
Pta.7598 unigene cluster
Pta.2091 unigene cluster
Mtr.2748 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Ppa.3917 unigene cluster
Pta.24201 unigene cluster
Lsa.11335 unigene cluster
Ghi.4846 unigene cluster
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
 
Sequence: HX03N10r
submitter of sequence: Dr. Nils Stein
length: 557
priming: 5-prime
GenBankAc: BQ470721
IPK CR-EST: HX03N10r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX03N10r
CGGCACGAGG CTCCCCCCTC CCTGCATCGT CGCGGAACAG TGCTCCCACC GCCGGCGCCA         60
TGAGTAAGTT GCAGAGCGAT GCCCTGAGGG AGGCTATCAC AAACATTACC AATGACTGCC        120
GTGAGAAGAA CCGCAAGTTC GTTGAAACCA TTGAGCTGCA GATTGGTCTG AAGAACTATG        180
ACCCGCAAAA GGACAAACGT TTCAGTGGCT CTGTTAAGCT TCCTCACATC CCTCGCCCAA        240
AGATGAGGGT TTGCATGCTT GGTGATGCCC AGCATGTTGA AGAGGCAGGG AAAATGGGCC        300
TTGACTGTAT GGATGTGGAA TCGCTGAAGA AGATGAACAA AAACAAGAAG CTAGTCAAGA        360
AGCTTGCAAA GAAGTATCAT GCTTTCCTTG CATCTGAGGC TATCATTAAG CAGATTCCCC        420
GACTTCTTGG ACCTGGTCTT AACAAGGCAG GAAAGTTTCC AACTTTGGTG TCTCACCAGG        480
AGTCCCTTGA GTCCAAGGTT AATGAGACCA AGGCCACTGT CAAGTTCCAG TTGAAAAAGG        540
TGCTTTGCAT GGGTGTT                                                       557
TypePositionOrientation
ORF 2 - 64: forward
ORF 60 - 557: forward
ORF 151 - 2: reverse
ORF 178 - 243: forward
ORF 275 - 3: reverse
ORF 379 - 531: forward
ORF 556 - 476: reverse
ORF 557 - 354: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
 
Sequence: HX03N10u
submitter of sequence: Dr. Nils Stein
length: 585
priming: 3-prime
GenBankAc: BQ665488
IPK CR-EST: HX03N10u 
HX03N10u
GGCACAGGGA CAACGGATAA AATGTCATGA AGTTCCAGAC AATAGAACAA ACCATCTCAG         60
TACAAGACAA TAATTGTCAC TCGACGCGCA CGAAAATTTG ATAATTGAGA AAGCAACAAA        120
AGTTCCCAAG TTGTCAATCT CCTGGAAATC CTAGAAGACT AGAGTCTAGA ACAACCGGTA        180
CACCTTTCCC ATTGTGCTCT TCAGGTACAG GCACCGCACG TTTTGCCAAT TCTTCTTCAA        240
CAGAGAGACA AGGAAGTTCA CGCTCATCTG CACGTTCTGG AAGATCTGCT TCTCCTCCAT        300
ACCCAGGTGG CCAACGGCAA CACCCATGCA AAGCACCTTT TTCAACTGGA ACTTGACAGT        360
GGCCTTGGTC TCATTAACCT TGGACTCAAG GGACTCCTGG TGAGACACCA AAGTTGGAAA        420
CTTTCCTGCC TTGTTAAGAC CAGGTCCAAG AAGTCGGGGA ATCTGCTTAA TGATAGCCTC        480
AGATGCAAGG AAAGCATGAT ACTTCTTTGC AAGCTTCTTG ACTAGCTTCT TGTTTTTGGT        540
CATCTTCTTC AGCGATTCCA CATCCATACA GTCAAGGCCC ATTTT                        585
TypePositionOrientation
ORF 2 - 70: forward
ORF 27 - 98: forward
ORF 326 - 400: forward
ORF 497 - 345: reverse
ORF 585 - 169: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
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