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Clone: HX02P07

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2034
  tissue: apex
  stage/tissue: apex (3-5 mm in size)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
LOC_Os01g55830.2 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Zm.9 unigene cluster
Sof.20462 unigene cluster
Sbi.17905 unigene cluster
Sbi.14146 unigene cluster
Os.97997 unigene cluster
Sof.17942 unigene cluster
Sbi.17875 unigene cluster
Sbi.18265 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HX02P07r
submitter of sequence: Dr. Nils Stein
length: 489
priming: 5-prime
GenBankAc: BQ470428
IPK CR-EST: HX02P07r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HX02P07r
CGGCACGAGG CCATCCCATC CCATCCGAGA AGAAGAGATA GAAAAAAAAA GGTTCGAGGG         60
CGTTGCGGAG TGAGAAGAGA CGAGATGGCT CCGGTGAAGC TGTACGGCGC GACCCTGTCG        120
TGGAACGTCA CCAGGTGCGT GGCGGCGCTG GAGGAGGCCG GCGTCGAGTA CGAGCTCGTC        180
CCCATCAACT TCAGCACCGG CGAGCACAAG AGCCCCGACC ACCTCGCCAG GAACCCCTTT        240
GGGCAGGTGC CAGCTTTGCA GGATGGTGAC TTGTATGTCT TCGAATCACG TGCTATTTGC        300
AAGTATGCGT GCCGCAAGAA CAAGCCAGAG CTGTTGAAGG AGGGCGACAT CAAGGAGTCA        360
GCAATGGTGG ATGTGTGGCT CGAGGTGGAG GCCCATCAGT ACACCGCCGC TCTGAGCCCC        420
ATTCTCTTCG AGTGCCTTAT CCATCCTATG CTTGNGGGAG CCACTGACCA GAAGGTCATC        480
GACGACAAC                                                                489
TypePositionOrientation
ORF 2 - 70: forward
ORF 85 - 489: forward
ORF 263 - 334: forward
ORF 350 - 3: reverse
ORF 488 - 399: reverse
ORF 489 - 361: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
 
Sequence: HX02P07u
submitter of sequence: Dr. Nils Stein
length: 569
priming: 3-prime
GenBankAc: BQ665305
IPK CR-EST: HX02P07u 
HX02P07u
GAGACAATAT CTGGGCACAA TTTATTATCA AACCAGAGCA CGGGAATGCT CATTTAAGTA         60
CAAGATTCGA CAGGATCGCA AGCACAAGCC TGAACATTCA ACACCAGGAC ACATCACAAG        120
AACACACACT AGGAAAGGCA CACATCGGGG GTGACACACC TCGGCTTATT TCGACGAACA        180
AGCCCCAGAA ACTAAGATCA TGGCTTCATC AGCGCAGCGA CCTTCTGGAC GGACGGCCTC        240
GTCAGCAGGT CAGTCCACCA GGCCTTCACA TGCGGGTAAG CGTCAAACAG AGATGCATAG        300
GGTGTGGCCG CCAAGCACAG GGTGACAGAC GCATGGTTAA GGTCCGCAAG GCTGAGGAAG        360
TCTCCAGCCA GGTACTTGGA CTTGGTGAGC CGCGCCTCGT ACACCGCCAG CACGTTCTTG        420
ATCTTAACAA GGTTGTCGTC GATGACCTTC TGGTCAGTGG CTCCCCCAAG CATAGGATGG        480
ATAAGGCACT CGAAGAGAAT GGGGCTCAGA GCGGCGGTGT ACTGATGGGC CTCCACCTCG        540
AGCCACACAT CCACCATTGC TGACTCCTT                                          569
TypePositionOrientation
ORF 2 - 151: forward
ORF 3 - 560: forward
ORF 114 - 31: reverse
ORF 200 - 322: forward
ORF 334 - 269: reverse
ORF 442 - 522: forward
ORF 567 - 457: reverse
ORF 569 - 201: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
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