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Clone: HV03M14

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2033
  tissue: germinating seeds
  stage/tissue: germinating seeds (48-96 h)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Roots were grown for two days on filter paper at room temperature. Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

Related with
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Zm.9 unigene cluster
Sof.20462 unigene cluster
Sbi.17905 unigene cluster
Sbi.14146 unigene cluster
 
Sequence: HV03M14r
submitter of sequence: Dr. Nils Stein
length: 162
priming: 5-prime
GenBankAc: BQ471889
IPK CR-EST: HV03M14r 
HV03M14r
CGGCACCGAG GGTCCCATCC CATCCCATCC GAGAAGAAGA GATAGAAAAA AAAAGGTTCG         60
AGGGCGTTGC GGAGTGAGAA GAGACGAGAT GGCTCCGGTG AAGCTGTACG GCGCGACCCT        120
GTCGTGGAAC GTCACCAGGT GCGTGGCGGC GCTGGAGGAG GC                           162
TypePositionOrientation
ORF 3 - 74: forward
ORF 89 - 160: forward
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
 
Sequence: HV03M14u
submitter of sequence: Dr. Nils Stein
length: 495
priming: 3-prime
GenBankAc: BQ664547
IPK CR-EST: HV03M14u 
HV03M14u
ATCTGGGCAC AATTTATTAT CAAACCAGAG CACGGGAATG CTCATTTAAG TACAAGATTC         60
GACAGGATCG CAAGCACAAG CCTGAACATT CAACACCAGG ACACATCACA AGAACACACA        120
CTAGGAAAGG CACACATCGG GGGTGACACA CCTCGGCTTA TTTCGACGAA CAAGCCCCAG        180
AAACTAAGAT CATGGCTTCA TCAGCGCAGC GACCTTCTGG ACGGACGGCC TCGTCAGCAG        240
GTCAGTCCAC CAGGCCTTCA CATGCGGGTA AGCGTCAAAC AGAGATGCAT AGGGTGTGGC        300
CGCCAAGCAC AGGGTGACAG ACGCATGGTT AAGGTCCGCA AGGCTGAGGA AGTCTCCAGC        360
CAGGTACTTG GACTTGGTGA GCCGCGCCTC GTACACCGCC AGCACGTTCT TGATCTTAAC        420
AAGGTTGTCG TCGATGACCT TCTGGTCAGT GGCTCCCCCA AGCATAGGAT GGATAAGGCA        480
CTCGAAGAGA ATGGG                                                         495
TypePositionOrientation
ORF 3 - 143: forward
ORF 106 - 23: reverse
ORF 192 - 314: forward
ORF 262 - 495: forward
ORF 494 - 261: reverse
ORF 495 - 193: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
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