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Clone: HS04B07

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2030
  tissue: embryo + scutellum
  stage/tissue: 0-16 hours after imbibition
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.

Related with
LOC_Os01g55830.1 annotated gene (TIGR5)
LOC_Os01g55830.2 annotated gene (TIGR5)
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Os.97997 unigene cluster
Sbi.17905 unigene cluster
Sbi.17875 unigene cluster
Zm.9 unigene cluster
Sof.17942 unigene cluster
Sof.20462 unigene cluster
Sbi.18265 unigene cluster
Sbi.14146 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HS04B07r
submitter of sequence: Dr. Nils Stein
length: 600
priming: 5-prime
GenBankAc: BQ467532
IPK CR-EST: HS04B07r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HS04B07r
CGGCACGAGG CATCCCATCC CATCCGAGAA GAAGAGATAG AAAAAAAAAG GTTCGAGGGC         60
GTTGCGGAGT GAGAAGAGAC GAGATGGCTC CGGTGAAGCT GTACGGCGCG ACCCTGTCGT        120
GGAACGTCAC CAGGTGCGTG GCGGCGCTGG AGGAGGCCGG CGTCGAGTAC GAGCTCGTCC        180
CCATCAACTT CAGCACCGGC GAGCACAAGA GCCCCGACCA CCTCGCCAGG AACCCCTTTG        240
GGCAGGTGCC AGCTTTGCAG GATGGTGACT TGTATGTCTT CGAATCACGT GCTATTTGCA        300
AGTATGCGTG CCGCAAGAAC AAGCCAGAGC TGTTGAAGGA GGGCGACATC AAGGAGTCAG        360
CAATGGTGGA TGTGTGGCTC GAGGTGGAGG CCCATCAGTA CACCGCCGCT CTGAGCCCCA        420
TTCTCTTCGA GTGCCTTATC CATCCTATGC TTGGGGGAGC CACTGACCAG AAGGTCATCG        480
ACGACAACCT TGTTAAGATC AAGAACGTGC TGGCGGTGTA CGAGGCGCGG CTCACCAAGT        540
CCAAGTACCT GGCTGGAGAC TTCCTCAGCC TTGCGGACCT TAACCATGCG TCTGTCACCC        600
TypePositionOrientation
ORF 1 - 69: forward
ORF 84 - 599: forward
ORF 262 - 333: forward
ORF 349 - 2: reverse
ORF 598 - 398: reverse
ORF 599 - 360: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
 
Sequence: HS04B07u
submitter of sequence: Dr. Nils Stein
length: 496
priming: 3-prime
GenBankAc: BQ662764
IPK CR-EST: HS04B07u 
HS04B07u
ATCTGGGCAC AATTTATTAT CAAACCAGAG CACGGGAATG CTCATTTAAG TACAAGATTC         60
GACAGGATCG CAAGCACAAG CCTGAACATT CAACACCAGG ACACATCACA AGAACACACA        120
CTAGGAAAGG CACACATCGG GGGTGACACA CCTCGGCTTA TTTCGACGAA CAAGCCCCAG        180
AAACTAAGAT CATGGCTTCA TCAGCGCAGC GACCTTCTGG ACGGACGGCC TCGTCAGCAG        240
GTCAGTCCAC CAGGCCTTCA CATGCGGGTA AGCGTCAAAC AGAGATGCAT AGGGTGTGGC        300
CGCCAAGCAC AGGGTGACAG ACGCATGGTT AAGGTCCGCA AGGCTGAGGA AGTCTCCAGC        360
CAGGTACTTG GACTTGGTGA GCCGCGCCTC GTACACCGCC AGCACGTTCT TGATCTTAAC        420
AAGGTTGTCG TCGATGACCT TCTGGTCAGT GGCTCCCCCA AGCATAGGAT GGATAAGGCA        480
CTCGAAGAGA ATGGGG                                                        496
TypePositionOrientation
ORF 3 - 143: forward
ORF 106 - 23: reverse
ORF 192 - 314: forward
ORF 262 - 495: forward
ORF 434 - 496: forward
ORF 494 - 261: reverse
ORF 495 - 193: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
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