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Clone: HF01M21

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2022
  tissue: caryopsis
  stage/tissue: developing caryopsis, 16-25 DAF (days after flowering)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  developing caryopsis, 16-25 DAF(days after flowing) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 940 bp

Related with
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
Sbi.19782 unigene cluster
Os.37672 unigene cluster
Bna.575 unigene cluster
Pta.24201 unigene cluster
Lja.14656 unigene cluster
Ppe.20380 unigene cluster
Tcc.3944 unigene cluster
Mtr.7684 unigene cluster
Ppe.5428 unigene cluster
Les.23394 unigene cluster
Gma.37744 unigene cluster
Ghi.4846 unigene cluster
Ptp.634 unigene cluster
Han.4573 unigene cluster
Pth.16449 unigene cluster
Han.1824 unigene cluster
Hv.22922 unigene cluster
Os.8864 unigene cluster
Os.11487 unigene cluster
Zm.138249 unigene cluster
Sof.5084 unigene cluster
Can.679 unigene cluster
Les.19407 unigene cluster
Bra.1732 unigene cluster
Bna.15209 unigene cluster
Hv.5459 unigene cluster
At.24240 unigene cluster
Zm.66965 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bna.4011 unigene cluster
Bol.3778 unigene cluster
Pth.5660 unigene cluster
Ptp.5768 unigene cluster
Sbi.837 unigene cluster
Bol.5797 unigene cluster
Bol.1747 unigene cluster
Vvi.9214 unigene cluster
Ptp.11065 unigene cluster
Hv.12839 unigene cluster
Nta.1653 unigene cluster
At.19006 unigene cluster
Can.3824 unigene cluster
Tcc.1562 unigene cluster
Pth.17313 unigene cluster
Bra.28765 unigene cluster
Nta.6843 unigene cluster
Zm.94920 unigene cluster
Les.3112 unigene cluster
Sof.20573 unigene cluster
Stu.2362 unigene cluster
Les.19882 unigene cluster
Ghi.18764 unigene cluster
At.7367 unigene cluster
Csi.5735 unigene cluster
Can.1546 unigene cluster
Ghi.5769 unigene cluster
Tcc.7897 unigene cluster
Ccl.2011 unigene cluster
Lja.541 unigene cluster
Lsa.5490 unigene cluster
Psi.8134 unigene cluster
Pgl.4932 unigene cluster
Gma.6491 unigene cluster
Pta.7598 unigene cluster
Pta.2091 unigene cluster
Mtr.2748 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Lsa.11335 unigene cluster
Lsa.14576 unigene cluster
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
 
Sequence: HF01M21w
submitter of sequence: Dr. Nils Stein
length: 677
priming: 3-prime
GenBankAc: BQ659815
IPK CR-EST: HF01M21w 
HF01M21w
ATCAATGGAC CAATGCTGAA TCTAGCGTTA AGCTGATGAT TGACAGCTAG CAGAACAGTA         60
GGAAGGCACA GGGACAACGG ATAAAATGTC ATGAAGTTCC AGACAATAGA ACAAACCATC        120
TCAGTACAAG ACAATAATTG TCACTCGACG CGCACGAAAA TTTGATAATT GAGAAAGCAA        180
CAAAAGTTCC CAAGTTGTCA ATCTCCTGGA AATCCTAGAA GACTAGAGTC TAGAACAACC        240
GGTACACCTT TCCCATTGTG CTCTTCAGGT ACAGGCACCG CACGTTTTGC CAATTCTTCT        300
TCAACAGAGA GACAAGGAAG TTCACGCTCA TCTGCACGTT CTGGAAGATC TGCTTCTCCT        360
CCATACCCAG GTGGCCAACG GCAACACCCA TGCAAAGCAC CTTTTTCAAC TGGAACTTGA        420
CAGTGGCCTT GGTCTCATTA ACCTTGGACT CAAGGGACTC CTGGTGAGAC ACCAAAGTTG        480
GAAACTTTCC TGCCTTGTTA AGACCAGGTC CAAGAAGTCG GGGAATCTGC TTAATGATAG        540
CCTCAGATGC AAGGAAAGCA TGATACTTCT TTGCAAGCTT CTTGACTAGC TTCTTGTTTT        600
TGTTCATCTT CTTCAGCGAT TCCACATCCA TACAGTCAAG GCCCATTTTC CCTGCCTCTT        660
CACATGCTGG GCATCAC                                                       677
TypePositionOrientation
ORF 91 - 162: forward
ORF 119 - 3: reverse
ORF 390 - 464: forward
ORF 561 - 409: reverse
ORF 646 - 233: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
 
Sequence: HF01M21T
submitter of sequence: Dr. Nils Stein
length: 651
priming: 5-prime
GenBankAc: BQ464269
IPK CR-EST: HF01M21T
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HF01M21T
CGGCACGAGG CCCTCCCCCC TCCCTGCATC GTCGCGGAAC AGTGCTCCCA CCGCCGGCGC         60
CATGAGTAAG TTGCAGAGCG ATGCCCTGAG GGAGGCTATC ACAAACATTA CCAATGACTG        120
CCGTGAGAAG AACCGCAAGT TCGTTGAAAC CATTGAGCTG CAGATTGGTC TGAAGAACTA        180
TGACCCGCAA AAGGACAAAC GTTTCAGTGG CTCTGTTAAG CTTCCTCACA TCCCTCGCCC        240
AAAGATGAGG GTTTGCATGC TTGGTGATGC CCAGCATGTT GAAGAGGCAG GGAAAATGGG        300
CCTTGACTGT ATGGATGTGG AATCGCTGAA GAAGATGAAC AAAAACAAGA AGCTAGTCAA        360
GAAGCTTGCA AAGAAGTATC ATGCTTTCCT TGCATCTGAG GCTATCATTA AGCAGATTCC        420
CCGACTTCTT GGACCTGGTC TTAACAAGGC AGGAAAGTTT CCAACTTTGG TGTCTCACCA        480
GGAGTCCCTT GAGTCCAAGG TTAATGAGAC CAAGGCCACT GTCAAGTTCC AGTTGAAAAA        540
GGTGCTTTGC ATGGGTGTTG CCGTTGGCCA CCTGNGTATG GAGGAGAAGC AGATCTTCCA        600
GAACGTGCAG ATGAGCGTGA ACTTCCTTGT CTCTCTGTTG AAGAAGAATT G                 651
TypePositionOrientation
ORF 1 - 66: forward
ORF 62 - 649: forward
ORF 153 - 1: reverse
ORF 180 - 245: forward
ORF 277 - 2: reverse
ORF 381 - 533: forward
ORF 649 - 356: reverse
ORF 650 - 525: reverse
ORF 651 - 478: reverse
Cluster Info:
Contig155.1 (81 members)
view Blast Searches
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