•Home    •Contact   •FAQs   •Imprint   •Login  
 
 
 
 
 
 
 
 
 
   
 
   
 
  
 
   

Help us improve the primary database. What do you think and where should we go in the future: https://www.surveymonkey.com/s/RK3GMPC?

 search 
 for 
Help

Clone: HD03B17

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Golden Promise
Library:
  lib name: IPKc 2020
  tissue: callus
  stage/tissue: callus (5-10 mm in diameter)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 1 kb

Related with
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
Hv.12839 unigene cluster
Zm.66965 unigene cluster
Hv.5459 unigene cluster
Os.37672 unigene cluster
Sof.20573 unigene cluster
Sbi.837 unigene cluster
Sbi.18522 unigene cluster
Zm.94920 unigene cluster
Zm.138249 unigene cluster
Os.8864 unigene cluster
Bra.1732 unigene cluster
Bna.15209 unigene cluster
Os.11487 unigene cluster
Vvi.9214 unigene cluster
Hv.22922 unigene cluster
Bol.5797 unigene cluster
Bol.1747 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Bra.28765 unigene cluster
Bna.4011 unigene cluster
Bol.3778 unigene cluster
At.24240 unigene cluster
At.19006 unigene cluster
Bna.16698 unigene cluster
At.7367 unigene cluster
Tcc.1562 unigene cluster
Can.679 unigene cluster
Stu.2362 unigene cluster
Csi.5735 unigene cluster
Ghi.18764 unigene cluster
Nta.6843 unigene cluster
Les.19407 unigene cluster
Ccl.2011 unigene cluster
Ghi.5769 unigene cluster
Tcc.3944 unigene cluster
Tcc.7897 unigene cluster
Lja.541 unigene cluster
Can.3824 unigene cluster
Ppa.3640 unigene cluster
Han.5727 unigene cluster
Pth.17313 unigene cluster
Hv.25032 unigene cluster
Sbi.18712 unigene cluster
Sof.5084 unigene cluster
Sbi.19782 unigene cluster
Bna.575 unigene cluster
Zm.127093 unigene cluster
Can.1546 unigene cluster
Ppe.20380 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Les.3112 unigene cluster
Pta.2091 unigene cluster
Bna.2003 unigene cluster
Ppe.5428 unigene cluster
Nta.23197 unigene cluster
Les.19882 unigene cluster
Mtr.7684 unigene cluster
Les.23394 unigene cluster
Lja.14656 unigene cluster
Lsa.5490 unigene cluster
Vvi.7697 unigene cluster
Nta.1653 unigene cluster
Mtr.2748 unigene cluster
Psi.8134 unigene cluster
Pgl.4932 unigene cluster
Mtr.16898 unigene cluster
Han.9639 unigene cluster
Pta.7598 unigene cluster
Gma.6491 unigene cluster
Pta.24201 unigene cluster
Nta.381 unigene cluster
Lsa.11335 unigene cluster
Ghi.4846 unigene cluster
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
 
Sequence: HD03B17r
submitter of sequence: Dr. Nils Stein
length: 521
priming: 5-prime
GenBankAc: BQ461832
IPK CR-EST: HD03B17r
Sequence features and Clone alignments (BLAST):
Click for Menu

Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HD03B17r
CGGCACGAGG CCAAAGTGAT GCTGTCAAGG ATGCCATCAC CCAGATTGTT GGGGAGGCCA         60
GAGAAAAGAA ACGCAAGTTC ACTGAGACTG TGGAGCTTCA GATTGGTCTC AAGAACTACG        120
ACCCACAAAA GGATAAACGT TTCAGTGGCT CTGTGAAGCT GCCCCATATC CCCCGCCCAA        180
AGATGAGGGT GTGCATGCTT GGTGATGCCC AGCACGTTGA CCAGGCTGAG AAAATGGGAC        240
TTGACTACAT GGATGTTGAA AGTCTTAAGA AGATGAACAA GAACAAGAAG CTTGTTAAGA        300
AGCTAGCCAA GAAGTACCAT GCTTTCCTGG CATCAGAGGC CATAATCAAG CAGATCCCTC        360
GTCTCCTTGG CCCTGGTCTT AACAAGGCAG GCAAGTTTCC CACTCTGGTA AGTCACCAGG        420
AATCTCTTGA AGCCAAGGTG AACGAGACAA AAGCTACAGT CAAGTTCCAG CTAAAGAAGG        480
TTCTTTGCAT GGGTGTTGCT GTGGGCAACT TGTCGATGGA C                            521
TypePositionOrientation
ORF 1 - 153: forward
ORF 167 - 3: reverse
ORF 183 - 521: forward
ORF 196 - 119: reverse
ORF 205 - 273: forward
ORF 343 - 248: reverse
ORF 520 - 416: reverse
Cluster Info:
Contig18.3 (64 members)
view Blast Searches
 
Sequence: HD03B17u
submitter of sequence: Dr. Nils Stein
length: 532
priming: 3-prime
GenBankAc: BQ658873
IPK CR-EST: HD03B17u 
HD03B17u
AACAGAAAAT GCAGTTCTAT TGCTCCCATG ATTTCTGTTA ACAAAAGACT AACAAGATAA         60
AATACTTGTT GATGTTCGCT TAGGTAAACA CAAAATTGGT ATCAGTGAAA CAGATCTAAT        120
CCAAAGACAA GCTCGGGTCG ACAGGACAGC CACTGGAGAA CACCAACTTA GAACACCCGT        180
ACACGCTTCC CCATGGTGCT CTTCACGTAC AGGCACCTCA CATTCTGCCA GTTCTTCTTC        240
AGGAGGGACA CAAGGAAGTT GACGCTCATT TGGATGTTTT GTTGGATCTG CTTCTCGTCC        300
ATCGACAAGT TGCCCACAGC AACACCCATG CAAAGAACCT TCTTTAGCTG GAACTTGACT        360
GTAGCTTTTG TCTCGTTCAC CTTGGCTTCA AGAGATTCCT GGTGACTTAC CAGAGTGGGA        420
AACTTGCCTG CCTTGTTAAG ACCAGGGCCA AGGAGACGAG GGATCTGCTT GATTATGGCC        480
TCTGATGCCA GGAAAGCATG GTACTTCTTG GCTAGCTTCT TAACAAGCTT CT                532
TypePositionOrientation
ORF 193 - 402: forward
ORF 530 - 171: reverse
Cluster Info:
Contig18.3 (64 members)
view Blast Searches
GabiPD Webmaster