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Clone: HA06F22

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os02g21660.3 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os01g55830.1 annotated gene (TIGR5)
Hv.5459 unigene cluster
Os.37672 unigene cluster
Zm.66965 unigene cluster
Hv.12839 unigene cluster
Zm.94920 unigene cluster
Sbi.837 unigene cluster
Sof.20573 unigene cluster
Bol.3778 unigene cluster
Sbi.18522 unigene cluster
Bra.1732 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
Hv.22922 unigene cluster
Bol.1747 unigene cluster
Bol.5797 unigene cluster
At.24240 unigene cluster
At.7367 unigene cluster
Les.19407 unigene cluster
Can.679 unigene cluster
Bna.4011 unigene cluster
Sof.5084 unigene cluster
Os.8864 unigene cluster
Hv.25032 unigene cluster
Ccl.2011 unigene cluster
Csi.5735 unigene cluster
Sbi.18712 unigene cluster
Tcc.1562 unigene cluster
Bna.575 unigene cluster
Bna.2003 unigene cluster
Stu.2362 unigene cluster
Tcc.7897 unigene cluster
Les.19882 unigene cluster
Ghi.18764 unigene cluster
Sbi.19782 unigene cluster
At.19006 unigene cluster
Ppa.3640 unigene cluster
Can.3824 unigene cluster
Nta.1653 unigene cluster
Bna.15209 unigene cluster
Vvi.7697 unigene cluster
Pta.2091 unigene cluster
Bra.28765 unigene cluster
Les.3112 unigene cluster
Han.5727 unigene cluster
Can.1546 unigene cluster
Nta.6843 unigene cluster
Lja.541 unigene cluster
Han.4573 unigene cluster
Ghi.4846 unigene cluster
Zm.138249 unigene cluster
Lja.14656 unigene cluster
Han.1824 unigene cluster
Os.11487 unigene cluster
Nta.23197 unigene cluster
Mtr.7684 unigene cluster
Vvi.9214 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Ghi.5769 unigene cluster
Ppe.20380 unigene cluster
Les.23394 unigene cluster
Mtr.2748 unigene cluster
Ppa.3917 unigene cluster
Psi.8134 unigene cluster
Gma.6491 unigene cluster
Pgl.4932 unigene cluster
Mtr.16898 unigene cluster
Lsa.5490 unigene cluster
Ptp.5768 unigene cluster
Ptp.11065 unigene cluster
Pta.7598 unigene cluster
Pta.24201 unigene cluster
Han.9639 unigene cluster
Pth.5660 unigene cluster
Tcc.3944 unigene cluster
Ppe.5428 unigene cluster
Pth.17313 unigene cluster
Mtr.17728 unigene cluster
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Zm.9 unigene cluster
Os.97997 unigene cluster
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LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
LOC_Os02g21660.3 annotated gene (MSU 6.1)
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HA06F22r
submitter of sequence: Dr. Nils Stein
length: 592
priming: 5-prime
GenBankAc: BQ460642
IPK CR-EST: HA06F22r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA06F22r
GAACAGGAAG TTCACTGAGA CCGTTGAGCT CCAGATCGGC CTCAAGAACT ATGATCCGCA         60
AAAGGACAAG CGTTTCAGCG GCTCCGTCAA GCTGCCCCAT GTCCCTCGCC CCAAGATGAG        120
GGTCTGCATG CTTGGTGATG CCCAGCACGT GGGAGAGGCT GAAAAGATTG GTCTGGATTC        180
CATGGACGTT GAAGCTCTTA AGAAGATGAA CAAGAACAAG AAGCTTGTCA AGAGGCTTGC        240
CAAGAAGTAC CATGCTTTCT TGGCCTCAGA GGCTATTATC AAGCAGATTC CACGTCTCCT        300
TGGTCCTGGT CTCAACAAGG CAGGAAAGTT CCCCACTCTG GTTTCTCACC AGGAGTCTCT        360
GGAAGCCAAG GTGAATGAGA CGAAAGCCAC AATCAAGTTC CAGCTCAAGA AGGTGCTGTG        420
CATGGGTGTC GCTGTTGGCA ACCTGTCGAT GGAGGAGAAG CAGATCCAGC AGAACATCCA        480
GATGAGCGTC AACTTCCTGG TTTCCCTGCT CAAGAAGAAC TGGCAGAATG TGAGGTGTCT        540
GTACATCAAG AGCACCATGG GGAAGCCTGT CCGGGTGTTC TAAGCTCGTG CC                592
TypePositionOrientation
ORF 2 - 580: forward
ORF 3 - 116: forward
ORF 138 - 206: forward
ORF 183 - 52: reverse
ORF 252 - 371: forward
ORF 253 - 2: reverse
ORF 375 - 482: forward
ORF 558 - 349: reverse
ORF 592 - 269: reverse
Cluster Info:
Contig531.1 (41 members)
view Blast Searches
 
Sequence: RUS06D10w
submitter of sequence: Dr. Nese Sreenivasulu
length: 612
priming: 3-prime
GenBankAc: FD521872
IPK CR-EST: RUS06D10w 
RUS06D10w
ATCTGGGCAC AATTTATTAT CAAACCAGAG CACGGGAATG CTCATTTAAG TACAAGATTC         60
GACAGGATCG CAAGCACAAG CCTGAACATT CAACACCAGG ACACATCACA AGAACACACA        120
CTAGGAAAGG CACACATCGG GGGTGACACA CCTCGGCTTA TTTCGACGAA CAAGCCCCAG        180
AAACTAAGAT CATGGCTTCA TCAGCGCAGC GACCTTCTGG ACGGACGGCC TCGTCAGCAG        240
GTCAGTCCAC CAGGCCTTCA CATGCGGGTA AGCGTCAAAC AGAGATGCAT AGGGTGTGGC        300
CGCCAAGCAC AGGGTGACAG ACGCATGGTT AAGGTCCGCA AGGCTGAGGA AGTCTCCAGC        360
CAGGTACTTG GACTTGGTGA GCCGCGCCTC GTACACCGCC AGCACGTTCT TGATCTTAAC        420
AAGGTTGTCG TCGATGACCT TCTGGTCAGT GGCTCCCCCA AGCATAGGAT GGATAAGGCA        480
CTCGAAGAGA ATGGGGCTCA GAGCGGCGGT GTACTGATGG GCCTCCACCT CGAGCCACAC        540
ATCCACCATT GCTGACTCCT TGATGTCGCC CTCCTTCAAC AGCTCTGGCT TGTTCTTGCG        600
GCACGCATAC TT                                                            612
TypePositionOrientation
ORF 1 - 552: forward
ORF 3 - 143: forward
ORF 106 - 23: reverse
ORF 192 - 314: forward
ORF 326 - 261: reverse
ORF 434 - 514: forward
ORF 610 - 449: reverse
ORF 612 - 193: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
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