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Clone: HA06F21

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
LOC_Os01g55830.2 annotated gene (TIGR5)
LOC_Os01g55830.1 annotated gene (TIGR5)
Sof.20462 unigene cluster
Sbi.17905 unigene cluster
Sbi.14146 unigene cluster
Hv.932 unigene cluster
Hv.3134 unigene cluster
Zm.81924 unigene cluster
Zm.9 unigene cluster
Os.97997 unigene cluster
Sof.17942 unigene cluster
Sbi.17875 unigene cluster
LOC_Os01g55830.1 annotated gene (MSU 6.1)
 
Sequence: HA06F21r
submitter of sequence: Dr. Nils Stein
length: 553
priming: 5-prime
GenBankAc: BQ460641
IPK CR-EST: HA06F21r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA06F21r
CGGCACGAGG CCCTCTCCCA CTCCACCTGC TCCATCGGAA GGGAGCAGCA GTGAGGCTCC         60
ATTGCCTATT TAGCCGAGAC CCCGAGCGCG GTCATTTCCC CATCCCGTCC CGTCCCATCC        120
CATCCCATCC GAGAAGAAGA GATAGAAAAA AAAAGGTTCG AGGGCGTTGC GGAGTGAGAA        180
GAGACGAGAT GGCTCCGGTG AAGCTGTACG GCGCGACCCT GTCGTGGAAC GTCACCAGGT        240
GCGTGGCGGC GCTGGAGGAG GCCGGCGTCG AGTACGAGCT CGTCCCCATC AACTTCAGCA        300
CCGGCGAGCA CAAGAGCCCC GACCACCTCG CCAGGAACCC CTTTGGGCAG GTGCCAGCTT        360
TGCAGGATGG TGACTTGTAT GTCTTCGAAT CACGTGCTAT TTGCAAGTAT GCGTGCCGCA        420
AGAACAAGCC AGAGCTGTTG AAGGAGGGCG ACATCAAGGA GTCAGCAATG GTGGATGTGT        480
GGCTCGAGGT GGAGGCCCAT CAGTACACCG NCGCTCTGAG CCCCATTCTC TTCGAGTGCC        540
TTATCCATCC TAT                                                           553
TypePositionOrientation
ORF 2 - 70: forward
ORF 189 - 551: forward
ORF 367 - 438: forward
ORF 454 - 95: reverse
ORF 551 - 465: reverse
Cluster Info:
Contig168.1 (88 members)
Representative for Uniset
view Blast Searches
 
Sequence: HA06F21u
submitter of sequence: Dr. Nils Stein
length: 481
priming: 3-prime
GenBankAc: BQ657942
IPK CR-EST: HA06F21u 
HA06F21u
ATATCTGGGC ACAATTTATT ATCAAACCAG AGCACGGGAA TGCTCATTTA AGTACAAGAT         60
TCGACAGGAT CGCAAGCACA AGCCTGAACA TTCAACACCA GGACACATCA CAAGAACACA        120
CACTAGGAAA GGCACACATC GGGGGTGACA CACCTCGGCT TATTTCGACG AACAAGCCCC        180
AGAAACTAAG ATCATGGCTT CATCAGCGCA GCGACCTTCT GGACGGACGG CCTCGTCAGC        240
AGGTCAGTCC ACCAGGCCTT CACATGCGGG TAAGCGTCAA ACAGAGATGC ATAGGGTGTG        300
GCCGCCAAGC ACAGGGTGAC AGACGCATGG TTAAGGTCCG CAAGGCTGAG GAAGTCTCCA        360
GCCAGGTACT TGGACTTGGT GAGCCGCGCC TCGTACACCG CCAGCACGTT CTTGATCTTA        420
ACAAGGTTGT CGTCGATGAC CTTCTGGTCA GTGGCTCCCC CAAGCATAGG ATGGATAAGG        480
C                                                                        481
TypePositionOrientation
ORF 2 - 145: forward
ORF 108 - 25: reverse
ORF 194 - 316: forward
ORF 264 - 479: forward
ORF 479 - 195: reverse
ORF 481 - 263: reverse
Cluster Info:
Contig168.1 (88 members)
view Blast Searches
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