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Clone: HA02B23

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2018
  tissue: embryosac
  stage/tissue: 0-7 DAP (days after pollination)
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  0-7 DAP(days after pollination) Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable. Average insert size is 1 kb

Related with
LOC_Os08g44450.2 annotated gene (TIGR5)
LOC_Os08g44450.1 annotated gene (TIGR5)
LOC_Os08g44380.1 annotated gene (TIGR5)
LOC_Os08g44380.2 annotated gene (TIGR5)
LOC_Os08g44380.3 annotated gene (TIGR5)
LOC_Os02g21660.2 annotated gene (TIGR5)
LOC_Os02g21660.1 annotated gene (TIGR5)
LOC_Os01g64090.1 annotated gene (TIGR5)
LOC_Os01g64090.2 annotated gene (TIGR5)
LOC_Os08g44450.3 annotated gene (TIGR5)
Hv.5459 unigene cluster
Os.37672 unigene cluster
Zm.66965 unigene cluster
Sof.20573 unigene cluster
Sbi.837 unigene cluster
Hv.12839 unigene cluster
Zm.94920 unigene cluster
Bol.3778 unigene cluster
Sbi.18712 unigene cluster
Bra.1732 unigene cluster
Hv.22922 unigene cluster
Bra.636 unigene cluster
Bra.1910 unigene cluster
At.7367 unigene cluster
Bol.1747 unigene cluster
Sof.5084 unigene cluster
Bol.5797 unigene cluster
Les.19407 unigene cluster
Can.679 unigene cluster
Ccl.2011 unigene cluster
Bna.575 unigene cluster
Bna.4011 unigene cluster
Csi.5735 unigene cluster
At.24240 unigene cluster
Stu.2362 unigene cluster
Bna.2003 unigene cluster
Tcc.1562 unigene cluster
Tcc.7897 unigene cluster
Les.19882 unigene cluster
Sbi.19782 unigene cluster
Os.8864 unigene cluster
Can.3824 unigene cluster
Vvi.7697 unigene cluster
Pta.2091 unigene cluster
Ppa.3640 unigene cluster
Nta.1653 unigene cluster
Les.3112 unigene cluster
At.19006 unigene cluster
Ghi.18764 unigene cluster
Can.1546 unigene cluster
Nta.23197 unigene cluster
Bra.28765 unigene cluster
Lja.541 unigene cluster
Han.5727 unigene cluster
Ghi.4846 unigene cluster
Han.4573 unigene cluster
Lja.14656 unigene cluster
Han.1824 unigene cluster
Os.11487 unigene cluster
Mtr.7684 unigene cluster
Ppa.3917 unigene cluster
Nta.6843 unigene cluster
Psi.6078 unigene cluster
Pgl.14502 unigene cluster
Vvi.9214 unigene cluster
Ppe.20380 unigene cluster
Les.23394 unigene cluster
Mtr.2748 unigene cluster
Psi.8134 unigene cluster
Pgl.4932 unigene cluster
Ghi.5769 unigene cluster
Pta.7598 unigene cluster
Lsa.5490 unigene cluster
Pta.24201 unigene cluster
Han.9639 unigene cluster
Tcc.3944 unigene cluster
Ppe.5428 unigene cluster
Mtr.17728 unigene cluster
LOC_Os08g44450.3 annotated gene (MSU 6.1)
LOC_Os08g44450.2 annotated gene (MSU 6.1)
LOC_Os08g44450.1 annotated gene (MSU 6.1)
LOC_Os08g44380.2 annotated gene (MSU 6.1)
LOC_Os08g44380.1 annotated gene (MSU 6.1)
LOC_Os02g21660.2 annotated gene (MSU 6.1)
LOC_Os02g21660.1 annotated gene (MSU 6.1)
LOC_Os01g64090.1 annotated gene (MSU 6.1)
 
Sequence: HA02B23r
submitter of sequence: Dr. Nils Stein
length: 623
priming: 5-prime
GenBankAc: BQ459160
IPK CR-EST: HA02B23r
Sequence features and Clone alignments (BLAST):
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Gene structure   UTR    start codon    stop codon    exon-exon border 
Clone alignments   forward    reverse    start    stop 
Protein domains   domain       
Click on sequence features to obtain additional information.
 
HA02B23r
CGGCACGAGG CTCACCGTGT TTCTTCCCCC CTTTCCCCGC AGGTGAATCG GCGACAGGCG         60
AATCGACCCA CCCCACCCCC CCGCGGCGAG CCATGAGTAA GCTGCAGACT GAGGCCCTCA        120
AGGAGGCCAT CACCCAGGTG GTGGCGGATG CCAAGGAGAA GAACAGGAAG TTCACTGAGA        180
CCGTTGAGCT CCAGATCGGC CTCAAGAACT ATGATCCGCA AAAGGACAAG CGTTTCAGCG        240
GCTCCGTCAA GCTGCCCCAT GTCCCTCGCC CCAAGATGAG GGTCTGCATG CTTGGTGATG        300
CCCAGCACGT GGGAGAGGCT GAAAAGATTG GTCTGGATTC CATGGACGTT GAAGCTCTTA        360
AGAAGATGAA CAAGAACAAG AAGCTTGTCA AGAGGCTTGC CAAGAAGTAC CATGCTTTCT        420
TGGCCTCAGA GGCTATTATC AAGCAGATTC CACGTCTCCT TGGTCCTGGT CTCAACAAGG        480
CAGGAAAGTT CCCCACTCTG GTTTCTCACC AGGAGTCTCT GGAAGCCAAG GTGAATGAGA        540
CGAAAGCCAC AATCAAGTTC CAGCTCAAGA AGGTGCTGTG CATGGGTGTC GCTGTTGGCA        600
ACCTGTCGAT GGAGGAGAAG CAG                                                623
TypePositionOrientation
ORF 1 - 93: forward
ORF 93 - 623: forward
ORF 130 - 2: reverse
ORF 148 - 276: forward
ORF 298 - 366: forward
ORF 343 - 212: reverse
ORF 412 - 531: forward
ORF 413 - 15: reverse
ORF 535 - 621: forward
ORF 622 - 509: reverse
ORF 623 - 429: reverse
Cluster Info:
Contig531.1 (41 members)
view Blast Searches
 
Sequence: HA02B23u
submitter of sequence: Dr. Nils Stein
length: 523
priming: 3-prime
GenBankAc: BQ656793
IPK CR-EST: HA02B23u 
HA02B23u
GAGGGTACAC AAGCTTTCAT CCACAACATA TTATAATAAC CGGTTCTCGC ATGATCTTGA         60
TGAGCATATT GTTCCATCAG AATGGCAACT TAGGAGAAAC AGCAGCAGTC TTTCATCATC        120
TTCATTCACC AGGACACGTG CAAAATTCGA TAAAAACTAC GACACGACAC TGACATATTT        180
AAGCATGAAC ATAGTAAGGA GGTCCAGTAT TGGAGGTAGC ATCGATATAG ACGGGCGGGC        240
AGGGGCACGA GCTTAGAACA CCCGGACAGG CTTCCCCATG GTGCTCTTGA TGTACAGACA        300
CCTCACATTC TGCCAGTTCT TCTTGAGCAG GGAAACCAGG AAGTTGACGC TCATCTGGAT        360
GTTCTGCTGG ATCTGCTTCT CCTCCATCGA CAGGTTGCCA ACAGCGACAC CCATGCACAG        420
CACCTTCTTG AGCTGGAACT TGATTGTGGC TTTCGTCTCA TTCACCTTGG CTTCCAGAGA        480
CTCCTGGTGA GAAACCAGAG TGGGGAACTT TCCTGCCTTG TTG                          523
TypePositionOrientation
ORF 2 - 253: forward
ORF 3 - 170: forward
ORF 77 - 3: reverse
ORF 222 - 160: reverse
ORF 278 - 487: forward
ORF 461 - 354: reverse
ORF 522 - 256: reverse
ORF 523 - 461: reverse
Cluster Info:
Contig531.1 (41 members)
view Blast Searches
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