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Clone: REGIo476H105D

Genotype (Genotype)
  species: Arabidopsis thaliana
  type: wildtype
  common name: thale cress
  cultivar: Columbia
Library:
  cloning vector: pDONR201 site_1: ; site_2:
  host: E. coli DH5alpha
  library description:
  In the context of the EU-funded project REGIA (QLG-CT11999-00876, coordinator Javier Paz-Ares), a set of transcription factor ORFs was generated. The ORFs were produced in a decentralized way in the labs of the participants. Most of the ORFs were generated by RT-PCR using cDNA from various A. thaliana tissues as a template. Initially, it was planned to use yeast recombination to move the ORFs from the cloning vectors into target constructs. For this reason, a number of the clones contain "REGIA tags" (RG_tag1: 5pr-AATTCCAGCTCACCACC-3pr; RG_tag2: 5pr-CATGGCAATTCCCGGGGATC-3pr). During the lifetime of the project, the GATEWAY system became available and finally all ORFs were transferred into GATEWAY vectors. At the end of the REGIA project, the clones were collected as plasmid DNA. For end-sequencing at the DNA core facility of the MPI of Plant Breeding Research (ADIS; head: Bernd Weisshaar), the plasmids were re-transformed into DH5alpha, arrayed into 96-well plates and new plasmid DNA was prepared. Re-transformation proved to be difficult because a number of clones displayed poor growth. Data submission has been handled by GabiPD (http://gabi.rzpd.de).

Material available:

Related with
AT1G06040.1 annotated gene (TAIR7)
AT1G06040.2 annotated gene (TAIR7)
At.70752 unigene cluster
Bra.21262 unigene cluster
Bna.10174 unigene cluster
Bra.16217 unigene cluster
Bna.4489 unigene cluster
At.23289 unigene cluster
AT1G06040.1 annotated gene (TAIR9)
AT1G06040.2 annotated gene (TAIR9)
 
Sequence: 80-L021131-065-005-H10-SeLB
submitter of sequence: Prof. Dr. Bernd Weisshaar
length: 896
tracefile: view (download)
GenBankAc: DR749977
description: AGI: AT1G06040; SeqAnalysis: full CDS correct; Translation: full good Data analysis performed in the frame of REGULATORS (Exploiting inter-species conservation in promoter sequences to identify regulators of reproductive development and physiological performance), a Trilateral Co-Operation in Plant Genomics between Spain (MCyT), France (GENOPLANTE) and Germany (GABI) coordinated by G. Coupland (coupland-ad-mpiz-koeln.mpg.de). Authors: Vincent Thareau (IBP-Orsay UMR8618 CNRS-UPS, thareau-ad-ibp.u-psud.fr) and Alain Lecharny (URGV-Evry UMR INRA-CNRS-UEVE, lecharny-ad-ibp.u-psud.fr). 
80-L021131-065-005-H10-SeLB
TTTATTTTGA CTGATAGTGA CCTGTTCGTT GCAACAACCT TTTAAGCCAT GCTTTCTTAT         60
AATGCCAACT TTGTACCCTT TACATGGGTT TTAGCCAAGA TCAGGGACAA TGAAGTGTTC        120
CTCATCATCA TCATCATATC TGGTCTCAAA CCTCGGCTTC TTGTGTGAAA CATTTGACTT        180
CATAGGTTTG TATGAATGAA CATGACCTAA ATGCGAAACA GAAAGCTCAG GAACTTCAGC        240
TGCAGGAAGA GCCTTGTCAT TAATCTGATC ACCGAAGAAT CCCATGTCTG AAAACCAATC        300
CAACTCCCCT GCCCCAAGAT CAAGCTGTCC TTTCTTGTCG GTGGATTCAA TATCAGAGAA        360
GTGAAAGAAA TCGTCAACAG CCCAGGGAAG TGGAGTAGCA GAAGAAGGTT GTTGTTGCTG        420
CTGGCTTGTG GATTTAGCAG GAATCTGATT AGCCTTCTGT TGGTTGTTGG AAGGCTCAGG        480
TTGATTCTTC TCAATTTCTT TACTACATAT AGTTGAGGTC AGAGCTACTT TGATCCCAGT        540
GGCTAAGAAC CTCTGGTGAT TAGCAGATCG AGAATTAGCC ACGTGGATGG ATTCATCGCA        600
GTCCCTGCAA AGCAGAGCTC TATCCTCTAC ACAGAAAATG AAAGCTGCCT TCTCTTGGCA        660
GATATCGCAA CGAGGGAATT TGGTGGAGAG GGAATTAAGA TGAAGACGTT GGTGCTTGCT        720
AGCGAGTTTG TTAGCGGCGT GAATCTCGAT GTCGCATTGA GGACAGAGAG CAGCTTCGTC        780
GGCGCAACAA ATCACCGTCG TCGGAGCTTT CTCACACACA TCACACTGTA TCTTCATGCA        840
GCCTGCTTTT TTGTACAAAG TTGGCATTAT AAAAAAGCAT TGCTCATCAA TTTGTT            896
view Blast Searches
 
Sequence: 80-L021127-065-005-H10-SeLA
submitter of sequence: Prof. Dr. Bernd Weisshaar
length: 615
tracefile: view (download)
GenBankAc: DR749976
description: AGI: AT1G06040; SeqAnalysis: full CDS correct; Translation: full good Data analysis performed in the frame of REGULATORS (Exploiting inter-species conservation in promoter sequences to identify regulators of reproductive development and physiological performance), a Trilateral Co-Operation in Plant Genomics between Spain (MCyT), France (GENOPLANTE) and Germany (GABI) coordinated by G. Coupland (coupland-ad-mpiz-koeln.mpg.de). Authors: Vincent Thareau (IBP-Orsay UMR8618 CNRS-UPS, thareau-ad-ibp.u-psud.fr) and Alain Lecharny (URGV-Evry UMR INRA-CNRS-UEVE, lecharny-ad-ibp.u-psud.fr). 
80-L021127-065-005-H10-SeLA
ACCTGTTCGT TGCACAAATT GATGAGCAAT GCTCTCATAT AAGGCCCACT TTGTACAAAA         60
AAGCAGGCTG CATGCTTATA CAGTGTGATG TGTGTGAGAA AGCTCCGGCG ACGGTGATTT        120
GTTGCGCCGA CGAAGCTGCT CTCTGTCCTC AATGCGACAT CGAGATTCAC GCCGCTAACA        180
AACTCGCTAG CAAGCACCAA CGTCTTCATC TTAATTCCCT CTCCACCAAA TTCCCTCGTT        240
GCGATATCTG CCAAGAGAAG GCAGCTTTCA TTTTCTGTGT AGAGGATAGA GCTCTGCTTT        300
GCAGGGACTG CGATGAATCC ATCCACGTGG CTAATTCTCG ATCTGCTAAT CACCAGAGGT        360
TCTTAGCCAC TGGGATCAAA GTAGCTCTGA CCTCAACTAT ATGTAGTAAA GAAATTGAGA        420
AGAATCAACC TGAGCCTTCC AACAACCAAC AGAAGGCTAA TCAGATTCCT GCTAAATCCA        480
CAAGCCAGCA GCAACAACAA CCTTCTTCTG CTACTCCACT TCCCTGGGCT GATGACGATT        540
TCTTTCACTT CTCTGATATT GAATCCACCG ACAAGAAAGG ACAGCTTGAT CTTGGGGCAG        600
GGGAGTTGGA TTGGT                                                         615
view Blast Searches
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