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Clone: HR01B11

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Barke
Library:
  lib name: IPKc 2029
  tissue: roots
  stage/tissue: grown on filter paper at 25 Grad C for 48 hours in petridish, 1-1.5 cm in length
  cloning vector: pBluescript SK+ site_1: EcoRI (5'-end of cDNA); site_2: XhoI (3'-end of cDNA)
  host: XL10-Gold
  antibiotic: Ampicillin
  library description:
  Due to a cloning artefact caused by the kit, in most cases the EcoRI site is NOT present, as well as the EcoRIadapter used for cloning. To excise the insert, restriction sites upstream EcoRI should be used (e.g. BamHI, SalI,PstI). NOTE: Also due to the cloning system used Blue/white selection for recombinats is not 100% reliable.Average insert size is 800 bp

Related with
Hv.8009 unigene cluster
 
Sequence: HR01B11r
submitter of sequence: Dr. Nils Stein
length: 225
priming: 5-prime
GenBankAc: BQ467837
IPK CR-EST: HR01B11r 
HR01B11r
GCCGCCGCGA ATTCCGCCGG CGTCCGCCCC GCGCGCGCGT GCTCTGCGCC CCCGTGTCGG         60
CCCCGGCGGA CCCCAGGACC TCCGGATCGG AGCCCCACGC GCCTCGCCAT GGCGGCCAAC        120
GCGCTCCAGG CCCAGCTCAA CGCGCTCCTC GCCTCCATGT TCGCCACGGG TATGGTGGAC        180
GACCAGTTCC AGCAGCTGCA GTCGCTGGAC TGCAGGCCTC GATCT                        225
TypePositionOrientation
ORF 109 - 225: forward
ORF 158 - 3: reverse
ORF 223 - 140: reverse
Cluster Info:
Contig3529.1 (9 members)
view Blast Searches
 
Sequence: HR01B11u
submitter of sequence: Dr. Nils Stein
length: 414
priming: 3-prime
GenBankAc: BQ661980
IPK CR-EST: HR01B11u 
HR01B11u
GGTATAAGGT TGACACGTAC ATCCAACACA GGGCAAAAAA GTTCCGTTTC AAGGGGTCGC         60
GAGCCCGGAG TTCCACCGCT GGAATCGGTC AATCCGGTTC TGCACACACA CAGCCGTTAC        120
ACTGCCTACA AACAAGTTTA AGGGATACAT ACACACTGCC CCCCCACCAT CATGTCAGGG        180
GTCACATGGA GCTAAGCTCC TTCTGCTTCA GTTTTTACTG CTACTCTACT TGGGACTGCA        240
GGCCTCGATC TGCTGCCCCA GCTGCATCAT GGTCTGGAAC TTGCTGCGCA CATCACAGAA        300
CTCACTCCTA ACAAGGGCCA ACGCCATGAG GCACCCATCT TTACTTTTTG CCTCATAGAA        360
CTGGCGGAAG TGCATGCAGG CGAGCTTCAC TTTCTGAGCG CCAACACTGG AGCT              414
TypePositionOrientation
ORF 1 - 138: forward
ORF 3 - 308: forward
ORF 150 - 52: reverse
ORF 269 - 355: forward
ORF 356 - 270: reverse
ORF 414 - 229: reverse
Cluster Info:
Contig3529.1 (9 members)
view Blast Searches
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