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Clone: HH04N09

Genotype (Genotype)
  species: Hordeum vulgare
  type: wildtype
  common name: barley
  cultivar: Sloop
Library:
  lib name: IPKc 2037
  tissue: coleoptile
  stage/tissue: coleoptile, 1 day old
  cloning vector: pSPORT site_1: SalI (5-end of cDNA); site_2: NotI (3-end of cDNA)
  host: DH10B
  antibiotic: Ampicillin
  library description:
  Due to the cloning system used blue/white selection for recombinants is not 100 % reliable. Average insert size is 1.3 kb.
 
Sequence: HH04N09w
submitter of sequence: Dr. Nils Stein
length: 399
priming: 5-prime
GenBankAc: CB863478
IPK CR-EST: HH04N09w 
HH04N09w
CACGCGTCCG CTCCAGTGTT GGCGCTCAGA AAGTGAAGCT CGCCTGCATG CACTTCCGCC         60
AGTTCTATGA GGCAAAAAGT AAAGATGGGT GCCTCATGGC GTTGGCCCTT GTTAGGAGTG        120
AGTTCTGTGA TGTGCGCAGC AAGTTCCAGA CCATGATGCA GCTGGGGCAG CAGATCGAGG        180
CCTGCAGTCC CAAGTAGAGT AGCAGTAAAA AGTGAAGCAG AAGGAGCTTA GCTCCATGTG        240
ACCCCTGACA TGATGGTGTG GGGGGCAGTG TGTATGTATC CCTTAAACTT GTTTGTAGGC        300
AGTGTAACGG GCTGTGTGTG TGCAGAACCG GACTGACCGA TTCCTGCGGT GGAACTCCGG        360
GCTCGCGACC CCTTGAAACG GAACTTGTTT GCCCTGTGT                               399
TypePositionOrientation
ORF 2 - 67: forward
ORF 3 - 194: forward
ORF 67 - 153: forward
ORF 237 - 115: reverse
ORF 250 - 333: forward
ORF 397 - 68: reverse
ORF 398 - 231: reverse
ORF 399 - 286: reverse
Cluster Info:
Contig3529.1 (9 members)
view Blast Searches
 
Sequence: HH04N09y
submitter of sequence: Dr. Nils Stein
length: 382
priming: 3-prime
GenBankAc: CB863738
IPK CR-EST: HH04N09y 
HH04N09y
ACACAGGGCA AACAAGTTCC GTTTCAAGGG GTCGCGAGCC CGGAGTTCCA CCGCAGGAAT         60
CGGTCAGTCC GGTTCTGCAC ACACACAGCC CGTTACACTG CCTACAAACA AGTTTAAGGG        120
ATACATACAC ACTGCCCCCC ACACCATCAT GTCAGGGGTC ACATGGAGCT AAGCTCCTTC        180
TGCTTCACTT TTTACTGCTA CTCTACTTGG GACTGCAGGC CTCGATCTGC TGCCCCAGCT        240
GCATCATGGT CTGGAACTTG CTGCGCACAT CACAGAACTC ACTCCTAACA AGGGCCAACG        300
CCATGAGGCA CCCATCTTTA CTTTTTGCCT CATAGAACTG GCGGAAGTGC ATGCAGGCGA        360
GCTTCACTTT CTGAGCGCCA AC                                                 382
TypePositionOrientation
ORF 1 - 114: forward
ORF 2 - 169: forward
ORF 3 - 332: forward
ORF 150 - 67: reverse
ORF 163 - 285: forward
ORF 333 - 247: reverse
ORF 382 - 206: reverse
Cluster Info:
Contig3529.1 (9 members)
view Blast Searches
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